Andrographolide gallate compound with antioxidant activity and preparation method of compound
A technology of andrographolide and antioxidant activity, which is applied in the fields of organic chemistry methods, anti-toxic agents, organic chemistry, etc., can solve the problems of andrographolide derivatives' stability decline and influence on pharmacological activity, and achieve significant antioxidant activity and structure Novel, high reaction efficiency effect
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Embodiment 1
[0030] (1) Weigh 15.0g of gallic acid and add it to 225mL of anhydrous carbon tetrachloride, reflux and dissolve for 30min, add 2~3 drops of N,N-dimethylformamide, and slowly add it at a temperature of -5~0℃ 78.2g of oxalyl chloride, raise the temperature to 80°C, reflux for 5h, the reaction is completed, and the excess oxalyl chloride can be removed by rotary evaporation;
[0031] (2) Weigh 10.0g of andrographolide in 100mL of anhydrous tetrahydrofuran, reflux and dissolve for 30min, add 18.4g of the product of step (1), then add 2 to 3 drops of N,N-dimethylformamide, and stir the reaction at room temperature After 5 hours, the reaction was completed, the solvent was concentrated under reduced pressure at 50°C, and dried over anhydrous sodium sulfate;
[0032] (3) Weigh 9.3g of the product of step (2) and dissolve it in 100mL of chloroform, add 5.6g of hydrofluoric acid, stir and react at room temperature for 7h, thin-layer chromatography shows no progress in the reaction, an...
experiment example 2
[0033] Experimental Example 2 Compounds of the present invention are tested for DPPH free radical scavenging ability
[0034] (1) Preparation of sample solution: Accurately weigh 4.7 mg of DPPH sample, dissolve it with absolute ethanol and dilute it to a 50 mL volumetric flask to obtain a DPPH solution with a concentration of 0.094 mg / mL. The andrographolide gallate compound was prepared into a 10mg / mL mother solution with absolute ethanol, stored at 4°C for later use, and the mother solution was diluted with absolute ethanol to a concentration of 1.20, 3.60, 14.4, 28.8, 12.0, 36.0, 144, 288 ( ppm) sample solution (experimental group) for scavenging DPPH free radical ability test.
[0035] Vitamin C sample solution (control group 1), andrographolide sample solution (control group 2), and gallic acid sample solution (control group 3) were prepared in the same way.
[0036] (2) Detection method: draw 0.1mL of sample solution and 0.1mL of DPPH solution into a test tube, mix well...
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