Composite microbial preparation for treating type 2 diabetes and preparation process thereof
A technology of complex microorganisms and type 2 diabetes, which is applied in the field of medical pathology, can solve problems such as vitamin B12 deficiency, abnormal blood sugar metabolism, and increase treatment costs, and achieve the goals of lowering blood sugar levels, high therapeutic effects, and reducing drug resistance and stress response Effect
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Embodiment 1
[0033] It includes three parts: maternal extraction sample, in vitro culture, and detection and quantitative cultivation. The maternal extraction sample part includes the following parts:
[0034] S1: Use endoscopic surgery to extract maternal gastrointestinal mucus for in vitro cultivation;
[0035] S2: Extract the anaerobic bacteria, Akkermansia, Clostridium tenenella and probiotics in the gastrointestinal mucus, mark and store them separately.
[0036] Include the following parts in the in vitro culture part:
[0037] N1: Place the extracted anaerobic bacteria, Akkermansia, Clostridium tenebilis and probiotics in slanted petri dishes for mass cultivation. After the cultivation is completed, store them in a refrigerator;
[0038] N2: Take maternal blood samples and divide them into several marked groups according to equal doses. Blood samples are also mixed for anaerobic bacteria, Akkermansia, Clostridium tenebilis and probiotics, and the measurement and marking of each mix...
Embodiment 2
[0041] The detection and quantitative cultivation part includes the following parts:
[0042] M1: Detection of biochemical indicators on maternal blood samples, including FBG, HDL-C, LDL-C, TC, TG, HbA1c and HOMA-IR;
[0043] M2: Mix the blood samples of the marked group with the compound microbial preparation, and after a period of refrigeration, test the biochemical indicators of the blood samples of the marked group again;
[0044] M3: Obtain the change value of the relevant biochemical indicators, select the optimal mixed group, and calculate the content of the compound microbial preparation according to the ratio of the drawn blood sample to the normal blood content of the mother*1:5.
[0045] Beneficial effects: the extracted microorganisms act on the patient's own blood sample, and detect various biochemical index parameters in the mixed blood sample, so that an optimal treatment plan can be obtained.
Embodiment 3
[0047] Mixed grouping in the N2 step, including the following combinations: anaerobic bacteria, Akkermansia, Clostridium tenebilis, probiotics, (anaerobic bacteria+Akkermansia), (anaerobic bacteria+Clostridium tenebilis ), (anaerobic bacteria + probiotics), (anaerobic bacteria + Akkermansia + Clostridium tenebilis), (anaerobic bacteria + Akkermansia + probiotics), (anaerobic bacteria + Clostridium tenebilis + probiotics), (Akkermansia + Clostridium tenebilis), (Akkermansia + probiotics), (Akkermansia + Clostridium tenebilis + probiotics) and (Clostridium tenebilis + probiotics ).
[0048] According to the combination above, list the parameter tables of various biochemical indicators, the table is as follows:
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[0050]
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[0052] In summary: in the present invention, for the treatment of type 2 diabetes, the method of microbial treatment is used, and the microorganisms used are derived from the patient itself. During the treatment process, the patient's d...
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