A 3D printed flaxseed hydrogel scaffold and preparation method and application
A flaxseed gum and flaxseed technology, applied in 3D object support structures, manufacturing tools, additive manufacturing, etc., can solve the problems of low cell activity, high swelling, high viscosity of konjac gum, etc., and achieve simple operation and low cost. , the effect of good fidelity
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Embodiment 1
[0047] This embodiment introduces a process of 3D printing linen hydrogel brackets and preparation methods and applications.
[0048] figure 1 The specific steps of the preparation method of the 3D printing linen gelatin stent provided for the invention, which shows a 3D printing linen seed hydrogel bracket and preparation method and application. The steps are divided into:
[0049] Step 1: Lineseed glue ingredients are dissolved in ultra -pure water and fully stirred at 60 ° C to obtain the linen seed glue solution;
[0050] Step 2: Slowly add methyl acrylic anhydride at a speed of 100ul / min at 50 ° C, and stir at 50 ° C for 1 hour;
[0051] Step 3: Drops of hydrogen hydrolysis to maintain the pH of the reaction system at 7.0, and the continuous reaction is 5h;
[0052] Step 4: Depending on the amount of methyl acrylic anhydride, the methalinide gel derivatives with different branches are obtained from different branches. Dried seed gum powder;
[0053] Step 5: PBS with a concen...
Embodiment 2
[0068] Based on the above embodiment 1, this embodiment 1 explores the scheme of the embodiment 1, and has obtained preferred preparation methods.
[0069] The present invention provides a 3D printed nail -based acrylic -based flaxseed gum stent, which includes the following steps:
[0070] Step 1: The linen seed gum raw material is dissolved in ultra -pure water with a concentration of 1 % W / V, and is fully stirred and dissolved at 60 ° C to obtain the flaxseed glue solution;
[0071] Step 2: At the temperature of 50 ° C temperature table, 100UL is slowly added to the permeal acrylic anhydride, and it lasts for 1 hour for 10 ° C;
[0072] Step 3: Drops of hydrogen hydrolysis to maintain the pH of the reaction system at 7.0, and the continuous reaction is 5h;
[0073] Step 4: Depending on the amount of methyl acrylic anhydride, the metharocylite gel derivatives with different branches are obtained. Dried linseed gum dried powder;
[0074] Step 5: Use a neutral cell culture liquid ...
Embodiment 3
[0080] Based on the above embodiment 2, the examples of this embodiment 2 are described in detail the 3D printing linen -seeding gel bracket with a 4 % W / V printing concentration in the embodiment 2.
[0081] Preparation 4 % W / V, 5 % W / V, 6 % W / V three different printing concentrations of 3D printing flaxseed gel gel brackets: Frozen -dried methyl acrylate -granite gel powder is caused by light The cell culture medium of the agent (0.1 % W / V) is completely dissolved and prepared to be prepared to be prepared to be prepared; after that, the income of the water gel or the compound with the bone marrow (1 × 107Cell / ml) compound Printing, through the accumulation of fibrous fibrous fibrous fibrous silk, forming a bracket, the printed bracket for ultraviolet light 365nm and 200mw / cm2 cross -linking 60s, which is the product.
[0082] Compare the three brackets and print parameters, and find that the number of layers printed can be printed more than 20 layers. Among them, 4 % (W / V) need...
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