Aptamer of methotrexate, aptamer derivative and application of aptamer and aptamer derivative

A technology of nucleic acid aptamer and methotrexate, applied in biological testing, biochemical equipment and methods, instruments, etc., can solve the problems of large toxic and side effects, narrow therapeutic window, endangering the life safety of patients, etc., and achieve small molecular weight, The effect of small batch variance and high stability
CN113249386AActive Publication Date: 2021-08-13EAST CHINA NORMAL UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
EAST CHINA NORMAL UNIV
Publication Date
2021-08-13

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Abstract

The invention discloses an aptamer of methotrexate, an aptamer derivative and application of the aptamer and the aptamer derivative. A nucleotide sequence of the aptamer comprises DNA molecules as shown in sequences 1-5. The aptamer can be a derivative obtained from various similar sequences with high homology or derivatives provided by the invention. A method for detecting the methotrexate is established by utilizing the specific binding effect of the aptamer HMX38 and the methotrexate for the first time. The aptamer and the derivative thereof can be used for preparing methotrexate detection probes and drug carriers or used for drug design and development, drug separation and purification and the like. The aptamer and the derivative thereof have the advantages of being capable of being combined with methotrexate with high specificity and high affinity, free of immunogenicity, capable of being chemically synthesized, good in biocompatibility, small in molecular weight, stable, easy to store and the like.
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Description

technical field

[0001] The present invention relates to a nucleic acid aptamer, nucleic acid aptamer derivatives and applications thereof, in particular to a nucleic acid aptamer capable of binding methotrexate, a nucleic acid aptamer derivative and its ability to recognize and bind methotrexate Application of pterin. Background technique

[0002] Methotrexate (MTX), a folic acid antagonist, is widely used in the clinical treatment of various cancers and inflammatory diseases. According to reports, nearly 700 kinds of cancer cells are sensitive to MTX, among which acute lymphoblastic leukemia cell lines and acute myeloid leukemia cell lines are the most sensitive to MTX. MTX can interfere with the synthesis of tetrahydrofolate, thereby inhibiting the synthesis of DNA and RNA in cells, thus playing an anti-tumor effect. However, MTX has the problems of narrow therapeutic window and large toxic and side effects. The adverse reactions induced by high-dose methotrexate (HDMTX)...

Claims

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