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Uvaria grandiflora botanical fungicide and preparation method and application thereof

A botanical fungicide, the technology of sansho seeds, applied in the directions of botanical equipment and methods, fungicides, plant growth regulators, etc., can solve the problem that the anti-phytopathogenic bacteria activity of sansho seeds and sanshoenone has not been reported, etc. Good control effect, strong bactericidal activity and broad antibacterial spectrum

Active Publication Date: 2021-08-17
HAINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

To sum up, a large number of medical experiments have shown that sanshocenone exhibits high-efficiency biological activities in anti-tumor and anti-tuberculosis, but the activity of sanshoperone and sanshotenone in anti-phytopathogenic bacteria has not been reported yet.

Method used

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  • Uvaria grandiflora botanical fungicide and preparation method and application thereof
  • Uvaria grandiflora botanical fungicide and preparation method and application thereof
  • Uvaria grandiflora botanical fungicide and preparation method and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] The preparation of embodiment 1-sanjiaozi whole plant methanol crude extract

[0032] Preparation of Crude Methanol Extract from the Whole Plant of Sanshochu

[0033]Cut the fresh Sansanthoxylum plant into small pieces, put it into a blast drying oven for drying at 50°C, and grind it into powder. Weigh 2.5kg of the powder and soak it in 20L methanol solution with a volume fraction of 99.5% for 72 hours each time. The soaking liquids were combined and filtered, and concentrated under vacuum at 45°C with a rotary evaporator to obtain the methanol crude extract of the whole plant of Sanshochu.

Embodiment 2

[0034] The preparation of embodiment 2-sanjiaozi whole plant methanol crude extract

[0035] Cut the fresh Sansanthoxylum plant into small pieces, put it in a blast drying oven to dry at 48°C, grind it into powder, weigh 2kg of the powder and soak it in 20L of methanol solution with a volume fraction of 99%, soak for 70 hours each time, soak twice, and soak The liquid was combined and filtered, and concentrated under vacuum at 40°C with a rotary evaporator to obtain a methanol crude extract of the whole plant of Sanshochu.

Embodiment 3

[0036] The preparation of embodiment 3-sanjiaozi whole plant methanol crude extract

[0037] Cut the fresh Sansanthoxylum plant into small pieces, dry them in a blast drying oven at 52°C, and grind them into powder. Weigh 3kg of the powder and soak them in 20L of methanol solution with a volume fraction of 99.5% for 75 hours each time. The solution was combined and filtered, and concentrated under vacuum at 50°C with a rotary evaporator to obtain crude methanol extract of the whole plant of Sanshochu;

[0038] (1) Antibacterial activity test in vitro

[0039] Adopt mycelia growth rate method, the Sansanthoxylum methanol crude extract that above embodiment 1~3 obtains carries out bactericidal activity detection.

[0040] Get respectively the capsicum methanol crude extract of embodiment 1~3 and be dissolved in DMF (0.1mL), then mix with potato dextrose agar (PDA; 9.9mL), finally be made into required concentration. The crude extract was tested for activity at a concentration ...

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Abstract

The invention provides a uvaria grandiflora botanical fungicide and a preparation method and application thereof, and the broad-spectrum botanical fungicide comprises a uvaria grandiflora whole plant extract and a compound uvaria grandiflora ketene obtained by separation and purification, and has the advantages of wide antibacterial spectrum, simple separation process and the like; potted plant bactericidal activity tests prove that the uvaria grandiflora extract has a good control effect on phytophthora capsici, cucumber downy mildew, potato late blight and cowpea powdery mildew and has a promoting effect on cucumber growth, and the prevention and treatment effect of uvaria grandiflora ketene on the cucumber downy mildew is equivalent to that of a chemical bactericide cyazofamid; meanwhile, field efficacy tests prove that the bactericidal activity of the uvaria grandiflora extract on cucumber powdery mildew is superior to that of a commercial bactericide Lunsen, and the uvaria grandiflora extract can be used as a potential botanical bactericide for preventing and controlling plant diseases.

Description

technical field [0001] The present invention relates to the technical field of fungicides, in particular to a sansanthoxylum plant-derived fungicide and its preparation method and application. Background technique [0002] With the improvement of people's awareness of environmental protection, the voice of advocating nature, protecting the environment, and paying attention to food safety is increasing day by day. Pollution-free biological pesticides and biological control technologies and concepts have gradually attracted people's attention. Natural fungicides isolated from plants have been successfully used to control plant diseases, and have advantages over chemically synthesized pesticides, such as containing new compounds that are not yet resistant to known plant pathogenic fungi, relatively fast biodegradation, and less residue , renewable materials, etc. At present, many plant extracts have been proven to have fungicidal activity against phytopathogenic fungi, such as...

Claims

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Application Information

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IPC IPC(8): A01N65/08A01N37/10A01P3/00C07C67/48C07C69/78
CPCA01N65/08A01N37/10C07C67/48A01N65/00C07C69/78A01P3/00A01N37/08
Inventor 孙然锋贺建国胡展谢佳
Owner HAINAN UNIVERSITY
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