Identification and application of human amniotic mesenchymal stem cell exocrine protein POSTN

An amniotic membrane mesenchymal and stromal cell technology, which can be used in medical preparations containing active ingredients, applications, peptide/protein components, etc., and can solve the problems that POSTN application research has not been in-depth and POSTN has been identified.
CN113262295AActive Publication Date: 2021-08-17中国医科大学
3 Cites 0 Cited by

Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
中国医科大学
Publication Date
2021-08-17

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention relates to application of human amniotic mesenchymal stem cell exocrine protein POSTN in preparation of a product for promoting spermatogonial stem cell proliferation and an identification method of the exocrine protein POSTN. The identification method comprises the following steps: collecting a human amniotic mesenchymal stem cell conditioned medium; and carrying out mass spectrometry on the exocrine protein of the amniotic mesenchymal stem cells in the conditioned culture medium, and screening out the high-expression protein POSTN. The exocrine proteome of the amniotic mesenchymal stem cells is identified, the results are analyzed and integrated, the spermatogonial stem cell proliferation positive factor POSTN is screened out, and a detailed foundation and a new thought are provided for subsequent research on amniotic stem cell paracrine effects. The POSTN provides a new cell factor for in-vitro proliferation of spermatogonial stem cells, and provides a direction for better excavating the characteristics of exocrine proteins of amniotic stem cells.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention relates to the field of biotechnology, in particular to the identification and application of human amniotic mesenchymal stem cell exocrine protein-periostin (POSTN). Background technique

[0002] Mesenchymal stem cells (MSCs) have been shown to secrete various autocrine / paracrine factors, including growth factors and cytokines, which play a major role in cell therapy. Human amniotic membranes are often discarded as biological waste after childbirth and become an important source of amniotic mesenchymal stem cells (hAMSCs). In addition, it has many other advantages such as anti-inflammatory, antibacterial, anti-angiogenic properties and low immunogenicity. HAMSC-secreted factors for cell-free therapy of acute brain injury. We found many proteins related to cell proliferation in hAMSCs conditioned medium (hAMSCs-CM).

[0003] Periostin (POSTN) is an extracellular matrix protein composed of 836 amino acids and about 90kD in size. POSTN ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More