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Microecological preparation for increasing number of mature follicles and litter size of mammals as well as preparation method and application of microecological preparation

A technology of probiotics and mature follicles, which is applied in the field of probiotics, can solve the problems of high reproductive performance intestinal microbial differences, etc., and achieve the goal of increasing the number of mature follicles and litters in mice, having a good application prospect, and promoting follicle development Effect

Active Publication Date: 2021-08-17
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Recently, Shao et al. (2020) and Uryu et al. (2020) used high-throughput microbiome studies to find that there were significant differences in the gut microbes of sows with high reproductive performance and low reproductive performance

Method used

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  • Microecological preparation for increasing number of mature follicles and litter size of mammals as well as preparation method and application of microecological preparation
  • Microecological preparation for increasing number of mature follicles and litter size of mammals as well as preparation method and application of microecological preparation

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Taking high-yield Meishan sows and relatively low-yield commercial Landrace × Large White (Long × Large) sows as the research objects, four key strains related to reproduction (Fibrobacter intestinalis, Lactobacillus mucosae , Bifidobacterium thermophilum and Ruminococcus flavefaciens);

[0031] The bacterial liquid preparation method of any of the above-mentioned strains is as follows:

[0032] 1) Recovery: Quickly recover any of the liquid nitrogen cryopreserved bacterial solutions of Fibrobacter intestinalis, Lactobacillus mucosae, Bifidobacterium thermophilum, and Ruminococcus flavefaciens in a 37°C water bath, and inoculate them into fresh liquid medium for enrichment culture. When it becomes turbid, use an inoculation loop to dip an appropriate amount of bacterial solution and inoculate it in a solid medium;

[0033]2) Enrichment and identification: After a single colony grows in the solid medium, pick a single colony and inoculate it into a fresh liquid medium f...

Embodiment 2

[0042] The method for preparing probiotics 2 in this embodiment 2 is basically the same as the preparation method in embodiment 1, except that:

[0043] In probiotics 2, the ratio of viable bacteria of Fibrobacter intestinalis, Lactobacillus mucosae, Bifidobacterium thermophilum and Ruminococcus flavefaciens was 1:0.1:0.1:0.1.

Embodiment 3

[0045] The method for preparing probiotics 3 in this embodiment 3 is basically the same as the preparation method in embodiment 1, except that:

[0046] In probiotics 3, the ratio of viable bacteria of Fibrobacter intestinalis, Lactobacillus mucosae, Bifidobacterium thermophilum and Ruminococcus flavefaciens was 1:10:10:10.

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Abstract

The invention discloses a microecological preparation for increasing the number of mature follicles and the number of litter size of mammals as well as a preparation method and application of the microecological preparation. The microecological preparation is prepared from four kinds of probiotics, namely,Fibrobacter intestinalis, Lactobacillus mucosae, Bifidobacterium thermophilum and Ruminococcus flavefaciens. The microecological preparation disclosed by the invention is simple in preparation process and relatively low in production cost, and has a relatively good application prospect. The microecological preparation has remarkable effects of promoting follicle development of mice and increasing the number of mature follicles and litter size of the mice.

Description

technical field [0001] The invention relates to a microecological preparation, in particular to a probiotic preparation for increasing the number of mature follicles and litter size of mammals, a preparation method and application thereof. Background technique [0002] Inbred mice have low fecundity, that is, low litter size. In the breeding and production of model animal mice, the reproductive efficiency of inbred mice is significantly lower than that of outbred mice. Compared with outbred mice, inbred mice have a highly consistent genetic background and have an irreplaceable advantage in scientific research related to genetic background. [0003] At present, non-genetic measures such as nutritional intervention are commonly used to improve the reproductive performance of inbred mice, but the effect is limited. Such as supplementing vitamins (folic acid) and trace element selenium, exogenous hormones to promote ovulation, embryo transfer and other assisted reproductive te...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23K10/18A23K50/50C12R1/225C12R1/01
CPCC12N1/20A23K10/18A23K50/50
Inventor 晏向华徐保阳马立保唐义梅周书仪
Owner HUAZHONG AGRI UNIV
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