crRNA combination for multiple detection of hereditary hearing loss, kit and method theref

A genetic deafness, multiple detection technology, applied in the direction of recombinant DNA technology, DNA / RNA fragments, biochemical equipment and methods, etc., can solve the problems of unfavorable large-scale genetic screening, limitation, low throughput, etc., to avoid Effects of aerosol pollution, cost reduction, and high sensitivity
CN113265457APending Publication Date: 2021-08-17上海抗码芯瑞生物科技有限公司
5 Cites 1 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
上海抗码芯瑞生物科技有限公司
Publication Date
2021-08-17

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention discloses a crRNA combination for multiple detection of hereditary hearing loss, a kit and a method thereof. The crRNA combination targets five SNP sites of deafness genes GJB2 and SLC26A4 at the same time, and comprises nucleotide sequences as shown in SEQ ID No.2, SEQ ID No.2, SEQ ID No.2, SEQ ID No.2, SEQ ID No.6, SEQ ID No.8 and SEQ ID No.10. According to the multiple detection kit for hereditary hearing loss, a Cas12a detection reaction system is arranged in a micropore of a pore plate, multiple RPA amplification is performed in a hollow honeycomb chip, and the hollow honeycomb chip and the bottom of the micropore are physically isolated by using a hollow gasket, so that two reactions of multiple RPA amplification and Cas12a detection are firstly isolated and then fused; one-step and multiple detection is realized, and aerosol pollution possibly caused by uncovering detection is also avoided. The method also realizes high-throughput detection, at most 96 samples are detected in one reaction, 5 SNP loci are distinguished at the same time, and the method is suitable for large-scale genetic screening.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention relates to the technical field of biological detection, in particular to the field of SNP detection, in particular to a crRNA combination, kit and method for multiple detection of hereditary deafness, and in particular to a crRNA combination for multiple detection of hereditary deafness based on CRISPR / Cas12a , kits and methods. Background technique

[0002] Deafness is a common clinical auditory nervous system defect disease, which can be caused by genetic and environmental factors, of which genetic factors account for about 60% (Morton CC, et al., 2006, N Engl J Med, 354(20): 2151– 2164), there are more than 100 deafness-causing genes discovered by human research ( http: / / hereditaryhearingloss.org / ), but the pathogenesis is mainly concentrated in hotspot mutations in several genes such as GJB2, SLC26A4, 12s rRNA, and GJB3. According to statistics, there are more than 20 million hearing-impaired people in China, 800,000 deaf children ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More