Bacterial agent used for processing organic garbage, and preparation method and utilization method of bacterial agent
A technology of organic waste and bacterial agents, applied in the direction of microorganism-based methods, biochemical equipment and methods, fixed on/in organic carriers, etc., can solve the waste of organic matter, reduce the calorific value of waste, and the secondary pollution of the environment, etc. problem, to achieve the effect of simple and effective treatment process, short fermentation cycle and no secondary pollution
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Embodiment 1
[0017] Take 30 parts of Bacillus halotolerant, 30 parts of Bacillus licheniformis, 30 parts of Bacillus natto, 10 parts of Azotobacter torus, and 10 parts of Pichia pastoris.
[0018] Specific culture method: Bacillus halotolerant, Bacillus licheniformis, Bacillus natto, and Azotobacter torus were cultured in LB based on shaking at 30°C for 18 hours. Pichia pastoris was cultured with YPD based on shaking at 30°C for 36 hours.
[0019] Specific solidification method: use straw powder as the immobilization carrier, and attach bacteria to the carrier to form a solid bacterial agent. The ratio of compound bacterial liquid: solid carrier is 1:5. The bacterial liquid and straw powder are put into the appropriate mixing device in proportion for immobilization treatment, and it can be completed after 48 hours. The concentration of effective bacteria is required to reach 1.5~9.9×10 13 CFU / g.
[0020] Specific treatment methods:
[0021] Put the compounded bacterial agent and organ...
Embodiment 2
[0025] Take 35 parts of Bacillus halotolerant, 30 parts of Bacillus licheniformis, 35 parts of Bacillus natto, 10 parts of Azotobacter torus, and 5 parts of Pichia pastoris.
[0026] Specific culture method: Bacillus halotolerant, Bacillus licheniformis, Bacillus natto, and Azotobacter torus were cultured in LB based on shaking at 30°C for 18 hours. Pichia pastoris was cultured with YPD based on shaking at 30°C for 36 hours.
[0027] 1. Specific solidification method: After the fermentation of the bacterial liquid is completed, filter the bacterial liquid, discard half of the filtrate, use straw powder as the immobilized carrier, and attach the bacteria to the carrier to form a solid bacterial agent. The ratio of composite bacteria liquid: solid carrier is 1:5. Put the bacteria liquid and straw powder in proportion to the appropriate mixing device for immobilization treatment, which can be completed after 24 hours. The concentration of effective bacteria is required to reach...
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