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Method for detecting protein selenium in natural selenium-rich agricultural products

A detection method and a technology for agricultural products, which are applied in the preparation of test samples, measuring devices, sampling, etc., can solve the problems affecting the reliability of test results, cumbersome microwave digestion operations, large selenium content detection errors, etc., and achieve new selenium-enriched foods The effect of promotion, extraction and detection is low cost and easy to operate

Pending Publication Date: 2021-08-24
SHAANXI UNIV OF SCI & TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The microwave digestion operation is cumbersome, takes a long time, and the sample is easy to lose
Dry ashing digestion will cause selenium in the food to be lost in the form of volatile compounds, resulting in a large error in the detection of selenium content and affecting the reliability of the test results

Method used

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  • Method for detecting protein selenium in natural selenium-rich agricultural products
  • Method for detecting protein selenium in natural selenium-rich agricultural products
  • Method for detecting protein selenium in natural selenium-rich agricultural products

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] A method for detecting water-soluble protein selenium in natural selenium-enriched agricultural products of the present invention comprises the following steps:

[0040] Step 1: Crush selenium-enriched black soybeans into powder, pass through a 100-mesh sieve, weigh 0.5g into a 50mL conical flask, add 5mL of ultrapure water, and ultrasonically extract at 40°C for 60min, then extract at a low temperature of 2°C The supernatant was collected by centrifugation, the above operation was repeated for the precipitate, and the supernatants extracted twice were combined for the separation of water-soluble selenoproteins.

[0041] Step 2, using 0.1 mol / L hydrochloric acid to adjust the pH of the extract to the isoelectric point of the water-soluble selenoprotein 4.0 to obtain a conditioning solution, and centrifuging the conditioning solution at a low temperature of 2°C to obtain the water-soluble selenoprotein.

[0042] Step 3: Add 20mL of ultrapure water, 5mL of nitric acid, an...

Embodiment 2

[0045] A method for detecting salt-soluble protein selenium in natural selenium-enriched agricultural products of the present invention comprises the following steps:

[0046] Step 1, crush selenium-enriched black beans into powder, pass through a 100-mesh sieve, weigh 0.5g into a 50mL Erlenmeyer flask, add 5mL of 0.5 mol / L NaCl solution, ultrasonically extract at 40°C for 60min, The supernatant was collected by centrifugation at a low temperature of 0°C, the above operation was repeated for the precipitate, and the supernatants extracted twice were combined for the separation of salt-soluble selenoproteins.

[0047] Step 2, using 0.1 mol / L hydrochloric acid to adjust the pH of the extract to the isoelectric point of 4.3 of the salt-soluble selenoprotein to obtain a regulating solution, and centrifuging the regulating solution at a low temperature of 0°C to obtain the salt-soluble selenoprotein.

[0048] Step 3: Add 20mL of ultrapure water, 5mL of nitric acid, and 3mL of hydro...

Embodiment 3

[0051] A method for detecting alcohol-soluble protein selenium in natural selenium-enriched agricultural products of the present invention comprises the following steps:

[0052] Step 1: Break selenium-enriched black beans into powder, pass through a 100-mesh sieve, weigh 0.5g into a 50mL Erlenmeyer flask, add 5mL of 75% ethanol solution, ultrasonically extract at 40°C for 60min, then extract at a low temperature of 4°C The supernatant was obtained by conditioned centrifugation, the above operation was repeated for the precipitate, and the supernatants extracted twice were combined for the separation of alcohol-soluble selenoproteins.

[0053] Step 2, using 0.1 mol / L hydrochloric acid to adjust the pH of the extract solution to the isoelectric point of alcohol-soluble selenoprotein 5.5 to obtain a regulating solution, and centrifuging the regulating solution at a low temperature of 4°C to obtain alcohol-soluble selenoprotein.

[0054] Step 3: Add 20mL of ultrapure water, 5mL o...

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Abstract

The invention relates to a method for detecting protein selenium in natural selenium-rich agricultural products. The method comprises the following steps: ultrasonically extracting powder obtained by crushing agricultural products with ultrapure water, centrifuging, separating and precipitating to obtain a precipitate and an extracting solution, and merging the extracting solutions obtained by treating the precipitate to obtain an extracting solution A; replacing the ultrapure water with a NaCl solution, an ethanol solution and a NaOH solution to obtain an extracting solution B, an extracting solution C and an extracting solution D; adjusting the pH of each extracting solution to an isoelectric point of water, salt, alcohol and alkali-soluble selenoprotein, and performing centrifugal separation to obtain corresponding selenoprotein; respectively adding an acidolysis system into the selenoproteins to carry out wet digestion, and then sequentially carrying out acid removal, cooling and volume metering to obtain corresponding protein selenium detection liquid; detecting the four detection solutions by inductively coupled plasma emission spectrometry, and substituting each emission spectrum intensity into a linear regression equation obtained by a selenium standard solution to obtain the concentrations of water-soluble protein selenium, salt-soluble protein selenium, alcohol-soluble protein selenium and alkali-soluble protein selenium in the natural selenium-rich agricultural product.

Description

technical field [0001] The invention relates to the technical field of protein selenium extraction and detection, in particular to a method for detecting protein selenium in natural selenium-enriched agricultural products. Background technique [0002] Selenium has biological functions such as scavenging free radicals in the human body, anti-oxidation, anti-aging, anti-tumor, prevention of myocardial infarction and cardiovascular and cerebrovascular diseases, prevention of cancer, and improvement of body immunity. The third largest micronutrient health element after iodine and zinc. The selenium in the human body must be ingested from the outside to meet its own health needs, and the lack of selenium will cause adverse effects on the body. The International Federation of Academic Organizations recommends that the appropriate daily dietary selenium requirements for adult men and women are 40 μg and 30 μg, respectively. The Chinese Nutrition Society recommends that the daily ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/626G01N1/28G01N1/44G01N1/38G01N1/34G01N1/42
CPCG01N27/626G01N1/28G01N1/44G01N1/38G01N1/286G01N1/34G01N1/42
Inventor 贾玮周媛媛夏曾润石琳祁蒙
Owner SHAANXI UNIV OF SCI & TECH