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Application of nanogold in preparation of medicine for treating shigella infectious enteritis and medicine preparation of nanogold

A technology for pharmaceutical preparations and shigella, which is applied in the field of biomedicine, can solve the problems of the application of nano-gold and the mechanism of action that have not been reported in the literature.

Pending Publication Date: 2021-08-31
XUZHOU MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although gold nanoparticles are involved in the field of medicine, there are no literature reports on the application and mechanism of gold nanoparticles in improving gillard infectious enteritis

Method used

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  • Application of nanogold in preparation of medicine for treating shigella infectious enteritis and medicine preparation of nanogold
  • Application of nanogold in preparation of medicine for treating shigella infectious enteritis and medicine preparation of nanogold
  • Application of nanogold in preparation of medicine for treating shigella infectious enteritis and medicine preparation of nanogold

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] In vitro inhibition experiment of gold nanoparticles on Shigella

[0025] 1. Experimental steps

[0026] The in vitro antibacterial experiments of gold nanoparticles involved in the following examples all adopt the following process:

[0027] Experimental material: gold nanoparticles;

[0028] The strain of Shigella flexneri is Shigella flexneri 2a 301 strain (sf301, which has been used as a reference strain of Shigella flexneri in China);

[0029] Clinical drug-resistant Shigella flexneri 2448 (RF-2448) and clinical drug-resistant Shigella flexneri II-1 (RF-II-1) were provided by the Affiliated Hospital of Xuzhou Medical University, and their drug sensitivity results are shown in Table 1 , Table 2; It can be seen from the table that RF-2448 and RF-II-1 are resistant to most of the antibiotics. Through the antibacterial experiment of nano-gold on these two clinical Shigella, explore the clinical treatment of nano-gold Potential value of Shigella.

[0030] Table 1 Dr...

Embodiment 2

[0045] Antibacterial activity of gold nanoparticles against Shigella

[0046] 1. Experimental steps

[0047] Shigella Sf301 was co-cultured with 0 μM and 100 μM AuNCs for 2 h, the supernatant was discarded by centrifugation, and washed twice with saline. After adding 100 μL of tissue fixation solution, place in a refrigerator at 4° C. for 12 hours, and wash with 0.1% phosphate buffered saline (PBS) three times, 10 minutes each time. Then dehydration treatment with ethanol solution with gradient concentration (25% ethanol / 50% ethanol / 70% ethanol / 80% ethanol / 90% ethanol / 95% ethanol), 15 min each time. Finally dried, coated, observed and photographed under SEM (hitachi-s4800, Japan).

[0048] 2. Experimental results

[0049] Such as figure 2 It can be seen from the scanning electron microscope images in the figure that, compared with the control group, nano-gold damages the Shigella cell membrane and disintegrates the bacterial structure. It shows that gold nanoparticles ca...

Embodiment 3

[0051] Therapeutic Effect of Nanogold on Shigella Infectious Enteritis

[0052] 1. Experimental steps

[0053] The establishment of the Shigella-induced mouse colitis model involved in the following examples all adopts the following process:

[0054] Experimental animals: male C57BL / 6 mice, weighing 17-18 g, specific pathogen-free (SPF), provided by Zhejiang Weitong Lihua Company. The mice were raised in the Experimental Animal Center of Xuzhou Medical University. The room temperature was 25°C and the humidity was 40-70%. They were given 12 hours of day and night alternating light source, standard laboratory sterilized feed, free to eat and drink, and adapted to the environment for three days before the experiment.

[0055] Such as image 3 As shown, the mice were divided into random control group (NC), Shigella infection group (SF), nano-gold treatment group (SF+AuNCs), 10 in each group. In the NC group, 200 μL of sterile water was intragastrically administered to each mou...

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Abstract

The invention discloses application of nanogold in preparation of a medicine for treating shigella infectious enteritis and a medicine preparation of the nanogold, and belongs to the field of biological medicine. Experiments show that in vitro, nanogold has relatively strong bacteriostatic activity on various strains, especially clinical drug-resistant bacteria, can obviously destroy the cell structure of shigella and reduce the biofilm activity of shigella; and in addition, the rise of reactive oxygen species (ROS) in cells can be induced, and cell membranes are destroyed so as to obviously kill shigella and relieve clinical symptoms of shigella enteritis. In vivo, the nanogold can relieve clinical symptoms of shigella flexneri enteritis, relieve intestinal injury caused by shigella infection and reduce shigella load in excrement. The application field of the nanogold is widened, an experimental basis is provided for clinical application of the nanogold in preparation of the medicine for treating shigella infectious enteritis, and the nanogold has a good application prospect and a huge potential value.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to the application of nano gold in the preparation of medicines for treating Shigella infectious enteritis and its pharmaceutical preparations. Background technique [0002] Shigella is a facultative anaerobic gram-negative bacillus and the main cause of bacillary dysentery, which has a wide range of effects worldwide, mainly in developing countries. According to reports from 1990 to 2009, about 125 million cases of bacillary dysentery occur in Asia every year, and the disease is extremely harmful to children. It is one of the main causes of child death and is the most widely distributed and highest incidence in my country Intestinal infectious disease. [0003] There are four serotypes of Shigella, and bacillary dysentery is mainly caused by Shigella flexneri. Shigella enters the human body through fecal-oral transmission, passes through the stomach and small intestine, and fi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K33/242A61P31/04A61P1/00B82Y5/00
CPCA61K33/242A61P31/04A61P1/00B82Y5/00Y02A50/30
Inventor 杨欢彭至玥吴小小单云洁李晓
Owner XUZHOU MEDICAL UNIV
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