Rapid extraction method of high-purity lentinan

A technology of lentinan and an extraction method, which is applied in the field of plant polysaccharide extraction, can solve the problems of unguaranteed product purity, reduced yield of final product, cumbersome steps, etc., and is beneficial to popularization and application, improved yield, and improved extraction efficiency. Effect

Inactive Publication Date: 2021-08-31
EAST CHINA UNIV OF SCI & TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] Patent CN 112094878.A provides a method for enzymatically extracting lentinan, which takes a long time, only the enzyme extraction step takes 12 hours, and requires strict control of pH and temperature, and the steps are cumbersome
[0008] It can be seen that the traditional polysaccharide extraction methods have many problems such as long storage time and low yield, and the purity of the product cannot be guaranteed, so further purification treatment is usually required, which leads to a further reduction in the yield of the final product

Method used

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  • Rapid extraction method of high-purity lentinan
  • Rapid extraction method of high-purity lentinan

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] The present invention is a kind of rapid extraction method of high-purity lentinan, comprising the following steps:

[0043] (1) freeze-dry the fruiting body of shiitake mushrooms, the drying temperature is -90°C, and the drying time is 24 hours. After crushing, pass through a 50-mesh sieve to obtain shiitake mushroom powder, which is soaked in 10 times deionized water for 30 minutes;

[0044] (2) Flash extraction, place the mushroom soaking liquid in a flash extractor, set the extraction voltage to 120V, extract time to 1min, extract 3 times, and extract at a temperature of 50°C for extraction.

[0045] (3) Centrifuge, after pouring out the solid-liquid mixture in the flash extractor, adopt a large-capacity centrifuge to centrifuge at a speed of 4000r / min for 10min.

[0046] (4) Alcohol precipitation: Concentrate the clear liquid to a material ratio of 1:3, add 3 times the volume of absolute ethanol to make the ethanol concentration about 75%, the alcohol precipitation...

Embodiment 2

[0050] The present invention is a kind of rapid extraction method of high-purity lentinan, comprising the following steps:

[0051] (1) Freeze-dry the fruiting body of Lentinus edodes, the drying temperature is -90°C, and the drying time is 24 hours. After crushing, pass through a 10-mesh sieve to obtain Lentinus edodes powder. Add 5 times deionized water to soak for 10 minutes

[0052] (2) Flash extraction, place the mushroom soaking liquid in a flash extractor, set the extraction voltage to 100V, the extraction time to 30s, the number of extractions once, and the extraction temperature to room temperature for extraction.

[0053] (3) centrifugation, after pouring out the solid-liquid mixture in the flash extractor, adopt a large-capacity centrifuge to centrifuge at a speed of 3000r / min for 10min.

[0054] (4) Alcohol precipitation: Concentrate the clear liquid to a material ratio of 1:1, add 1 volume of absolute ethanol to make the ethanol concentration about 50%, the alcoh...

Embodiment 3

[0058] The present invention is a kind of rapid extraction method of high-purity lentinan, comprising the following steps:

[0059] (1) Freeze-dry the fruiting body of Lentinus edodes, the drying temperature is -80° C., and the drying time is 12 hours. After crushing, pass through a 100-mesh sieve to obtain Lentinus edodes powder. Add 20 times of deionized water to soak for 60 minutes

[0060] (2) Flash extraction, place the mushroom soaking liquid in a flash extractor, set the extraction voltage to 150V, the extraction time to 3min, the number of extractions 5 times, and the extraction temperature to 80°C for extraction.

[0061] (3) Centrifuge, after pouring out the solid-liquid mixture in the flash extractor, adopt a large-capacity centrifuge to centrifuge at a speed of 10000r / min for 10min.

[0062] (4) Alcohol precipitation: Concentrate the clear liquid to a material ratio of 1:5, add 4 times the volume of absolute ethanol to make the ethanol concentration about 80%, the...

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Abstract

The invention relates to the field of lentinan, in particular to a rapid extraction method of high-purity lentinan. According to the invention, fresh shiitake mushrooms are subjected to freeze drying, smashing, soaking, flash extraction, centrifugation of an extract, alcohol precipitation, ultrasonic redissolution, centrifugation and freeze drying, so the lentinan is obtained. The method comprises the following specific steps: raw material preparation and pretreatment: weighing fresh shiitake mushrooms, freeze-drying the fresh shiitake mushrooms, and conducting crushing and soaking; flash extraction: putting a mushroom soaking solution into a flash extractor for extraction; centrifuging: centrifuging the extract to obtain a supernatant; alcohol precipitation: adding absolute ethyl alcohol into the extract, and conducting centrifuging to obtain a polysaccharide precipitate; redissolving: adding deionized water into the polysaccharide precipitate, and carrying out ultrasonic dissolving to obtain extractum; and freezing and drying: freezing and drying the extractum to obtain the lentinan. According to the invention, extraction time is short, product purity is high, the extraction efficiency of the lentinan is improved, and application and popularization of the lentinan are facilitated.

Description

technical field [0001] The invention relates to the field of plant polysaccharide extraction, in particular to a rapid extraction method of lentinan. Background technique [0002] Lentinus edodes belongs to Basidiomycetes and Agaricaceae. It is an edible fungus with a long history. It often grows on fallen logs of broad-leaved trees. Spring and winter are its main growth periods. Or scattered and so on. Shiitake mushrooms are delicious and have a unique smell. They are known as the "King of Mountain Treasures". The earliest record of artificial cultivation in my country can be traced back to 800 years ago. [0003] Lentinan is a plant polysaccharide extracted from shiitake mushrooms with specific biological activity. It is the most significant pharmacologically active ingredient in shiitake mushrooms. Anti-aging, anti-radiation and other functions, it is a recognized immune enhancer and interferon in the world. [0004] However, the content of lentinan in the fruiting bod...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
CPCC08B37/0003
Inventor 胡坪周斌闫海宽付志博赵凌昊张敏马纯昶范哲欢韩奥轩任凯铭
Owner EAST CHINA UNIV OF SCI & TECH
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