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Application of DUSP5 in preparation of medicine for preventing, relieving and/or treating osteoporosis

An osteoporosis and drug technology, applied in the field of gene function and application, can solve problems such as no reports of DUSP5, and achieve the effects of broad development and application prospects, small size, and safe pharmacological functions.

Pending Publication Date: 2021-09-03
PEKING UNIV SCHOOL OF STOMATOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

DUSP5 is a member of the DUSPs family. Studies have shown that DUSP5 can relieve joint inflammation by inhibiting osteoclastosis. However, there is no literature report on DUSP5 related to osteogenic differentiation, and there is no report on DUSP5 in the preparation of drugs that promote bone formation.

Method used

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  • Application of DUSP5 in preparation of medicine for preventing, relieving and/or treating osteoporosis
  • Application of DUSP5 in preparation of medicine for preventing, relieving and/or treating osteoporosis
  • Application of DUSP5 in preparation of medicine for preventing, relieving and/or treating osteoporosis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1 Animal experiments prove that DUSP5 promotes the restoration of osteogenic differentiation ability of bone marrow mesenchymal cells in osteoporotic mice

[0027] The experimental process includes the following aspects:

[0028] 1. Construct animal models of mice with ovarian deprivation (OVX)-induced osteoporosis group and sham operation (SHAM) control group.

[0029] (1) Test materials: sterile gauze, iodophor, 75% alcohol, 1% pentobarbital sodium, 1ml syringe, ophthalmic forceps, ophthalmic scissors, hemostatic forceps, digging spoon, 5-0 absorbable suture, needle holder device, angle needle.

[0030] (2) Grouping of animals: A total of 48 C57BL / 6 mice aged 6-8 weeks were randomly divided into 4 groups. The specific groups are as follows: 16 rats in the sham operation group (SHAM); 16 rats in the ovariectomized surgery group (OVX); 8 rats in the ovariectomy + empty lentivirus tail vein injection group (OVX+vector); Eight rats in the virus intraperitoneal ...

Embodiment 2

[0136] Example 2 In vitro experiments prove that the junctional domain of DUSP5 is a key regulatory site for promoting osteogenic differentiation of bone marrow mesenchymal stem cells

[0137] The experimental process includes the following aspects:

[0138] 1. Induce the osteogenic differentiation of hBMMSCs. Through qRT-PCR and western blot experiments, it was found that during the osteogenesis process, the expression of DUSP5 increased significantly with the increase of OSX and RUNX2 expression (see image 3 A). It indicated that DUSP5 was involved in regulating the osteogenic differentiation process of hBMMSCs.

[0139] The primer sequences used in the qRT-PCR experiments are shown in Table 5 below

[0140] Table 5 qRT-PCR primer sequences

[0141]

[0142]Construction of DUSP5 stable knockdown and overexpression cell lines (lentivirus was purchased from Suzhou Gemma Co., Ltd., the control group (NC) and knockdown sequences are shown in Table 6 below, and the DUSP5 o...

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Abstract

The invention discloses an application of bispecific phosphatase DUSP5 in preventing, relieving and / or treating osteoporosis. It is found that a DUSP5 gene has the effect of treating the osteoporosis. Based on the effect of DUSP5 in treating the osteoporosis diseases, DUSP5 can be used for preparing medicines for preventing, relieving and / or treating the osteoporosis and / or other osteogenic dysfunction diseases. The connection structural domain based on the DUSP5 is a key regulation and control part for promoting osteogenic differentiation of the bone marrow mesenchymal stem cells, and the osteogenic dysfunction diseases are expected to be treated more conveniently and effectively at lower cost by only synthesizing the connection structural domain polypeptide fragment or analogue of the DUSP5 subsequently.

Description

technical field [0001] The present invention relates to the field of gene function and application, and specifically relates to the application of dual specificity phosphatase 5 (Dualspecificity phosphatase 5, DUSP5) as a target gene in the preparation of drugs for preventing, alleviating and / or treating osteoporosis. Background technique [0002] Osteoblast-mediated bone formation and osteoclast-mediated bone resorption jointly regulate the maintenance of bone homeostasis. Some disease factors and age-related changes can lead to decreased bone formation or increased bone destruction, resulting in a decrease in bone density and bone mass, and damage to bone microstructure, resulting in osteoporosis and an increased risk of fracture. In China, osteoporosis has leaped to the seventh place among common diseases and frequently-occurring diseases, and the incidence rate is increasing year by year. According to the etiology, osteoporosis is divided into primary and secondary oste...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K45/00A61K48/00A61P19/10C12Q1/6883G01N33/573
CPCA61K45/00A61K48/005C12Q1/6883G01N33/573A61P19/10C12Q2600/158G01N2800/108
Inventor 周永胜张萍刘云松吕珑薇刘学姣张晓
Owner PEKING UNIV SCHOOL OF STOMATOLOGY
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