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Silk fibroin/hydroxyapatite composite material and its preparation method and application

A technology of hydroxyapatite and silk fibroin, which is applied in the fields of clinical medicine and material science, can solve the problems of insufficient mechanical properties, uneven dispersion of hydroxyapatite, inability to process large-size bone nails, etc., and achieve obvious osteoinductivity , the effect of promoting bone defect recovery

Active Publication Date: 2022-05-13
HUBEI SAILUO BIOLOGICAL MATERIAL CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Aiming at the problem that the existing silk fibroin / hydroxyapatite composite materials cannot be processed into large-size bone nails, the inventors conducted in-depth research and found that due to the dispersion of hydroxyapatite in the large-size silk fibroin / hydroxyapatite composite materials Inhomogeneous, resulting in insufficient mechanical properties, unable to be processed into large-size bone screws

Method used

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  • Silk fibroin/hydroxyapatite composite material and its preparation method and application
  • Silk fibroin/hydroxyapatite composite material and its preparation method and application
  • Silk fibroin/hydroxyapatite composite material and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] (1) Take 5g of silk fibroin protein powder, add hydroxyapatite nanoparticles, and use a vortex mixer and an ultrasonic oscillator to mix thoroughly. Wherein, the hydroxyapatite nanoparticles account for 0 wt% to 40 wt% of the total amount of the hydroxyapatite nanoparticles and the silk fibroin.

[0050] (2) According to the mixture of 35g of hydroxyapatite nanoparticles and silk fibroin per 100mL of hexafluoroisopropanol, the mixture of hydroxyapatite nanoparticles and silk fibroin powder is evenly dispersed in the hexafluoroisopropanol In isopropanol, keep it in an oven at 55°C for 0.5h to completely dissolve hydroxyapatite nanoparticles and silk fibroin powder, and remove air bubbles to obtain hydroxyapatite nanoparticles, silk fibroin, hexafluoro Mixture of isopropanol.

[0051] (3) Take the mixture out of the oven, add 5g of silk fibroin powder and the same amount of hydroxyapatite nanoparticles as in step (1), and add the same amount of hexafluoride as in step (2...

Embodiment 2

[0063] Get the rod (nHA / SF-31%) that the hydroxyapatite nanoparticle prepared in Example 1 accounts for 31wt% of the total amount of hydroxyapatite nanoparticle and silk fibroin, cut it into powder, and perform Fourier Leaf infrared spectroscopy detection to study the mechanism of silk fibroin performance enhancement after methanol treatment. At 1658cm- 1 , l545 cm- 1 and 1242cm- 1 The absorption peaks at the positions belong to the characteristic absorption peaks of amide I, amide II and amide III of the dry silk fibroin molecule (Silk), indicating that its secondary structure is a random coil; after methanol regeneration, the three characteristic peaks are transferred to 1634cm- 1 、1520cm- 1 and 1230cm- 1 , indicating that silk fibroin transforms from random coils to β-sheets, which is beneficial to increase the strength of silk fibroin / hydroxyapatite composites. In addition, in the infrared spectrum of silk fibroin / hydroxyapatite composites at 603cm- 1 and 565cm- 1 ...

Embodiment 3

[0065]Take the nHA / SF-31% screw prepared in Example 1, culture it at the ankle bone of rabbits, track the degradation of the nHA / SF-31% screw, and find that after culturing at the ankle bone of rabbits for 2 months, the nHA / SF-31 The gap between the % screw and the host bone tissue was significantly reduced, and no inflammatory cells were observed during the degradation of the nHA / SF-31% screw, indicating that the degradation products of silk fibroin / hydroxyapatite composites can be absorbed by the human body. The analysis found that the degradation products of the hydroxyapatite / silk fibroin composite material provided by the present invention in the human body are all non-toxic and non-irritating products: the degradation products of silk fibroin are amino acids and water, which can be absorbed by the human body; Apatite is the main component of human bone, and its degradation products are phosphate and calcium ions, which are non-toxic and can be metabolized out of the body....

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Abstract

The invention relates to a silk fibroin / hydroxyapatite composite material and its preparation method and application. First, the mixture of hydroxyapatite nanoparticles, silk fibroin and hexafluoroisopropanol is kept warm at 50-60°C At least 1 hour to obtain a silk fibroin / hydroxyapatite solution; pour the silk fibroin / hydroxyapatite solution into a long cylindrical mold; the two ends of the long cylindrical mold are A-end and B-end respectively; at room temperature, Make end A open and end B closed, immerse the mold opening upwards in methanol vertically, and let it stand for at least 2 days; turn the mold upside down, make A end closed, B end open, and immerse the mold opening upwards vertically in methanol In the process, let it stand for at least 2 days; remove the mold, ventilate and dry, and obtain the silk fibroin / hydroxyapatite composite material. The silk fibroin / hydroxyapatite composite material prepared by the invention has uniform strength, good mechanical properties and obvious osteoinductivity.

Description

technical field [0001] The invention belongs to the fields of material science and clinical medicine, and relates to a silk fibroin / hydroxyapatite composite material and a preparation method and application thereof. Background technique [0002] Fracture refers to the partial or complete interruption or loss of the integrity or continuity of bone or cartilage tissue when bone or cartilage tissue is subjected to violent action. Deformity, dysfunction, and abnormal activities are typical symptoms of this disease. Complications such as shock may occur in patients with severe fractures, even life-threatening. It is usually treated by surgical implantation of fracture fixation material. The characteristics that an ideal bone repair material should have are: (1) biocompatibility: it can be directly combined with bone without preventing the normal activity of bone cells on its surface or interfering with the natural regeneration process of bone cells around it; (2) Mechanical tol...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L31/12A61L31/14
CPCA61L31/127A61L31/148A61L31/146C08L89/00A61L2430/02A61L2400/12A61L27/58A61L27/46A61L27/56
Inventor 吴琳潘宇林杰涵
Owner HUBEI SAILUO BIOLOGICAL MATERIAL CO LTD
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