Application of endophytic fungus fo-r20 in controlling rice panicle blast
An endophytic fungus, rice technology, applied in the field of application in the prevention and treatment of rice panicle blast, to achieve the effect of enhancing disease resistance
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Embodiment 1
[0029] Example 1 Isolation and identification of endophytic fungi FO-R20
[0030] 1. Isolation and purification of endophytic fungi FO-R20
[0031] The endophytic fungus FO-R20 was isolated from the roots of wild rice verruca yunnanensis (collected from Xishuangbanna, Yunnan province, China). The specific separation method is as follows: First, the roots of wild rice are continuously washed with tap water to carefully remove soil particles and appendages. Healthy root tissues were selected for surface disinfection, soaked in 75% ethanol for 1-2 minutes, 1% sodium hypochlorite for 4-5 minutes, and then washed with sterile deionized water for 3 times. The root tissue pieces were cut into 0.5 cm pieces, and then placed on a 2% malt powder agar medium (MEA, malt extractagar, OXOID; 50 mg / L of chloramphenicol was added to the medium to inhibit the growth of endophytic bacteria) and incubated on a plate. Incubate in the dark at 25°C. On the 5th day of culture, endophytic fungal h...
Embodiment 2
[0059] Example 2 The role of FO-R20 strain coating agent in the control of rice panicle blast
[0060] Tested plants: Oryza sativa L., a conventional variety, Zhejing 88.
[0061] 1. FO-R20 strain culture and liquid fermentation
[0062] The FO-R20 strain stored on the filter paper was inoculated on potato dextrose agar (PDA) solid medium for activation culture at 25°C in the dark for 7 days. Use a hole punch with a diameter of 0.5 cm to punch out the bacterial cake, inoculate the bacterial cake (5 pieces) into a conical flask containing 500 ml of liquid fermentation medium, and place it in a shaker (25°C, rotating speed 150) for 7 days. Then the liquid fermentation broth is vacuum filtered to remove the medium to obtain mycelium. 0.1 g of mycelium was weighed, and the moisture content was determined to be 80%, in order to calculate the dry weight of the mycelium.
[0063] PDA medium: glucose 20g / L, potato 200g / L, agar 15g / L. Weigh the required potatoes (200 g / L) according...
Embodiment 3
[0081] Example 3 The role of FO-R20 solid bacterial fertilizer in the control of rice panicle blast
[0082] Tested plants: Oryza sativa L., a conventional variety, Zhejing 88.
[0083] 1. FO-R20 strain culture and fermentation
[0084] The FO-R20 strain stored on the filter paper was inoculated on potato dextrose agar (PDA) solid medium for activation culture at 25°C in the dark for 7 days. Use a hole punch with a diameter of 0.5 cm to punch out the bacterial cake, inoculate the bacterial cake (5 pieces) into a conical flask containing 500 ml of liquid fermentation medium, and place it in a shaker (25°C, rotating speed 150) for 7 days. Then the liquid fermentation broth was inoculated into a culture bottle containing sterile wheat grains (500g grains / bottle, 100ml of fermentation broth: 500g grains), and cultured in a dark incubator at 25°C for 10-15d, until the mycelium grew covered with wheat Granules, spare.
[0085] PDA medium: glucose 20g / L, potato 200g / L, agar 15g / L....
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