Method for improving water solubility of astaxanthin and protecting astaxanthin from oxidative degradation of Fe<2+>
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A technology for oxidative degradation and astaxanthin, applied in skin care preparations, non-active ingredients of polymer compounds, cosmetics, etc.
Active Publication Date: 2021-09-10
CHINA AGRI UNIV
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[0009] Currently, ferritin is used to enhance the water solubility of astaxanthin and protect astaxanthin from Fe 2+ Oxidative degradation technology has not been reported at home and abroad
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[0028] A preparation method of astaxanthin-astaxanthin complex, comprising the steps of:
[0029] a) Prepare an astaxanthin solution with a concentration of 0.25-3.0 mmol / L, that is, weigh 0.0036 g of astaxanthin and dissolve it in 2.0 mL-24.0 mL of dimethyl sulfoxide / ethanol / acetone;
[0030] b) separating and purifying the shrimp ferritin, and preparing a shrimp ferritin solution with a concentration of 0.5-20 μmol / L.
[0031]c) Slowly stir the shrimp ferritin solution prepared in step b, then slowly drop the astaxanthin solution prepared in step a into the shrimp ferritin solution, the molar ratio of shrimp ferritin to green pigment is 1:10~1: 200;
[0032] d) Slowly stirring and reacting, the reaction time is 0.5 to 2 hours, and the reaction temperature is 15°C to 35°C to obtain an astaxanthin-prastoferrin complex solution;
[0033] An astaxanthin-astaxanthin complex prepared according to the preparation method, the final combination ratio of astaxanthin and astaxanthin ...
Embodiment
[0039] Preparation of Astaxanthin-Astaxanthin Complex:
[0040] a) preparing an astaxanthin solution with a concentration of 1.0 mmol / L, and dissolving it in dimethyl sulfoxide;
[0041] b) separating and purifying shrimp ferritin, and preparing a shrimp ferritin solution with a concentration of 1.0 μmol / L;
[0042] c) Slowly stir the shrimp ferritin solution prepared in step b, then slowly add the astaxanthin solution prepared in step a into the shrimp ferritin solution, 2 μL each time, drop 10 times, the astaxanthin and astaxanthin solution The molar ratio is 1:20;
[0043] d) Slowly stirring and reacting, the reaction time is 0.5h, and the reaction temperature is 20°C to obtain an astaxanthin-astiferrin complex solution;
[0044] Detection of Astaxanthin-Astaxanthin Complexes of the Invention from Fe 2+ Ability to induce oxidative degradation.
[0045] The Fe 2+ Induced astaxanthin oxidative damage. First, dilute astaxanthin (1.0mmol / L, 10μL) into 1.0mL MOPS buffer or...
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Abstract
The invention relates to a method for improving water solubility of astaxanthin and protecting the astaxanthin from oxidative degradation of Fe<2+>. The method comprises the following steps that (a), an astaxanthin solution is prepared, and the astaxanthin solution is dissolved in dimethyl sulfoxide/ethanol/acetone; (b), shrimp ferritin is separated and purified, and a shrimp ferritin solution with the concentration of 0.5-20 [mu]mol/L is prepared; (c), the shrimp ferritin solution is slowly stirred, and then an astaxanthin solution is slowly dropwise added into the shrimp ferritin solution; and (d), slowly stirring and reacting are carried out to obtain an astaxanthin-shrimp ferritin compound solution. The obtained solution passes through a 0.45 [mu]m filter membrane to obtain the astaxanthin-shrimp ferritin compound solution. The astaxanthin-astaxanthin compound prepared by the method is bright orange red, has good water solubility and stability, can protect the astaxanthin from oxidative degradation of Fe<2+>, is beneficial to processing and storage of the astaxanthin, and widens the use condition and range of the astaxanthin.
Description
technical field [0001] The invention relates to a method for improving the water solubility of astaxanthin and protecting it from Fe 2+ Oxidative degradation method, which can not only improve the water solubility of astaxanthin, but also protect astaxanthin from Fe 2+ Oxidative degradation belongs to the protection technology of functionally active small molecules. Background technique [0002] Astaxanthin (3,3′-dihydroxy-4,4′-diketo-β,β′-carotene), molecular formula C 40 h 52 o 4 , with a relative molecular weight of 596.86, is a natural carotenoid with keto-terpene unsaturated structure, which widely exists in marine animals and plants, such as algae, salmon, trout, shrimp and crab. There are 2 β-ionone rings and 11 conjugated double bonds in the molecular structure of astaxanthin, which has strong antioxidant properties and good physiological activity, and can effectively quench singlet oxygen, scavenge oxygen free radicals, prevent At the same time, it has extraord...
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