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Medicinal preparation for treating Alzheimer disease

A technology for Alzheimer's disease and pharmaceutical preparations, which is applied in the field of biomedicine and can solve problems such as difficult and accurate diagnosis of patients

Active Publication Date: 2021-09-10
舒培健康科学有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the diagnosis of Alzheimer's disease is mainly based on the patient's clinical manifestations, neuropsychological scale assessment, and brain imaging examination. Patients with atypical symptoms are more difficult to achieve an accurate diagnosis

Method used

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  • Medicinal preparation for treating Alzheimer disease
  • Medicinal preparation for treating Alzheimer disease
  • Medicinal preparation for treating Alzheimer disease

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1 Obtaining peripheral blood mononuclear cells

[0026] (1) Collect peripheral blood from 45 cases of Alzheimer's disease (AD group) and 45 normal people (NC group);

[0027] (2) Take a 15ml centrifuge tube, add 5ml of human peripheral blood lymphocyte separation medium, and then use a pipette gun to carefully draw 5ml of peripheral blood and slowly add it along the wall of the centrifuge tube;

[0028] (3) Centrifuge at 800g in a centrifuge for 25 minutes. After centrifugation, the liquid is divided into 4 layers from top to bottom, namely: plasma layer, PBMCs layer, transparent separation layer, and red blood cell layer;

[0029] (4) Carefully pipette the PBMCs layer into a 15ml enzyme-free centrifuge tube, add 10ml PBS buffer solution to wash the PBMCs layer, and place it in a centrifuge at 250g for 10min;

[0030] (5) Discard the supernatant, add 5ml PBS buffer to resuspend the cells, place in a centrifuge, and centrifuge at 250g for 10min;

[0031] (6) Di...

Embodiment 2

[0032] Example 2 Peripheral Blood Mononuclear Cell RNA Extraction

[0033] (1) Add 1ml of Trizol lysis buffer to the peripheral blood mononuclear cells obtained in Example 1, and use a pipette to repeatedly pipette so that the cells can be fully lysed;

[0034] (2) Transfer the lysed cells to an RNase-free 1.5ml EP tube and let stand at room temperature for 5 minutes;

[0035] (3) Add 200ul of chloroform to the tube, shake vigorously for 15s, and let stand at room temperature for 2min;

[0036] (4) Place the EP tube in a pre-cooled 4°C high-speed centrifuge and centrifuge at 12000rpm for 15 minutes. After taking it out, the sample is divided into three layers. Carefully absorb the aqueous phase of the upper layer into a new EP tube. Be careful not to Inhale the middle layer;

[0037] (5) Add an equal volume of pre-cooled isopropanol, mix thoroughly by inversion, and place at room temperature for 10 minutes;

[0038] (6) Place the EP tube in a pre-cooled 4°C high-speed centr...

Embodiment 3

[0040] Example 3 Reverse transcription reaction of total RNA

[0041] (1) Genomic DNA removal reaction, the reagent system is as follows:

[0042] Table 1 Reaction system for removing genomic DNA

[0043] Reagent dosage 5×gDNA Eraser Buffer 2.0μl gDNA Eraser 1.0μl Total RNA 1.0μg RNase Free dH2O Up to 10μl

[0044] Set the reaction conditions: Gently blow and beat to mix, centrifuge for 15s, put into PCR instrument at 42°C for 2min, 4°C ∞;

[0045] (2) Reverse transcription reaction, the reagent system is as follows:

[0046] Table 2 Reverse transcription reaction system

[0047]

[0048] Set the reaction conditions: Gently pipette to mix, centrifuge for 15s, put into PCR instrument at 37°C for 15min, 85°C for 5s, 4°C∞.

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Abstract

The invention provides a medicinal preparation for diagnosing Alzheimer's disease, and belongs to the technical field of biomedicine. The pharmaceutical preparation comprises an LINC01365 silencing agent and a pharmaceutical carrier, the LINC01365 silencing agent is a small interfering RNA, and the sequence of the small interfering RNA is as shown in SEQ ID NO. 10 and SEQ ID NO. 11. The small interfering RNA can effectively promote proliferation and differentiation of human neural stem cells.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a pharmaceutical preparation for treating Alzheimer's disease. Background technique [0002] Alzheimer's disease is the most common degenerative disease of the nervous system. With the increasing number of elderly people in my country, the number of patients with Alzheimer's disease is also increasing accordingly. Patients with Alzheimer's disease initially manifest as a decline in short-term memory. With the deepening of the disease, patients will develop long-term memory impairment and impairments in multiple cognitive areas such as language, execution, and logical reasoning. Ultimately, the patient's family and Society creates a heavy burden. [0003] At present, the diagnosis of Alzheimer's disease is mainly based on the patient's clinical manifestations, neuropsychological scale assessment, and brain imaging examination. Patients with atypical symptoms are ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K48/00A61K31/713A61P25/28
CPCA61K31/713A61P25/28
Inventor 不公告发明人
Owner 舒培健康科学有限公司