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Method for extracting and preparing selenium polysaccharide with high antioxidant activity from selenium-rich passion fruit peel

A technology of passion fruit peel and oxidation activity, which is applied in the field of food processing and can solve the problems of unfavorable retention of polysaccharide nutrients, long time-consuming, long cycle, etc.

Active Publication Date: 2021-09-10
GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the process of processing fruit juice, passion fruit peel is often discarded as a by-product, causing great waste, so it is necessary to find a method that can fully utilize the organic selenium in passion fruit peel
Passion fruit peel contains a large amount of selenium-bound polysaccharides, which have good anti-oxidation and hypoglycemic activities. However, due to the traditional method of extracting polysaccharides, ultrasonic-assisted hot water extraction is often used, and the efficiency is low and the loss of organic selenium is large.
The traditional method of extracting polysaccharides in hot water has the disadvantages of low yield, long cycle, and high energy consumption, which is not conducive to retaining the nutritional content of polysaccharides. Although ultrasonic, microwave, enzymatic hydrolysis and other treatment methods can improve the extraction rate of polysaccharides to a certain extent , and can maintain the physiological and biochemical characteristics of the sample to a certain extent, but the operation is cumbersome and time-consuming

Method used

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  • Method for extracting and preparing selenium polysaccharide with high antioxidant activity from selenium-rich passion fruit peel

Examples

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Effect test

Embodiment 1

[0029] A method for extracting and preparing selenium polysaccharides with high antioxidant activity from selenium-enriched passion fruit peel, comprising the following steps:

[0030] 1. Selenium-enriched passion fruit, cut in half with a knife, dig out the pulp containing seeds with a pulper (or by hand), wash the remaining passion fruit skin, dry it in a constant temperature drying oven at 45°C, and crush it with a Chinese herbal medicine grinder Pass through a 60-mesh sieve to obtain the selenium-enriched passion fruit peel powder.

[0031] 2. Take 60 g of selenium-enriched passion fruit peel powder and dissolve it in water to obtain passion fruit peel powder solution.

[0032] 3. Centrifuge the passion fruit peel powder solution with a large-capacity swing-off centrifuge at a centrifugal speed of 5500r / min for 15 minutes, and discard the supernatant. Add purified water to the centrifuged sediment according to the material-to-liquid ratio of 1:10, and use a colloid mill f...

Embodiment 2

[0037] A method for extracting and preparing selenium polysaccharides with high antioxidant activity from selenium-enriched passion fruit peel, comprising the following steps:

[0038] 1. Selenium-enriched passion fruit, cut in half with a knife, dig out the pulp containing seeds with a pulper (or by hand), wash the remaining passion fruit skin, dry it in a constant temperature drying oven at 45°C, and crush it with a Chinese herbal medicine grinder Pass through a 60-mesh sieve to obtain the selenium-enriched passion fruit peel powder.

[0039] 2. Selenium-enriched passion fruit peel powder 60g, sodium carboxymethylcellulose (CMC-Na) 1.0g, NaNO 3 0.6g, K 2 HPO 4 0.2g, KCl 0.1g, pure water 200ml, according to the inoculation amount of Cladosporium, according to 2% inoculum, insert the activated mixed seed solution, place in a constant temperature shaking incubator, set the speed at 150r / min, and the temperature at 35 ℃, ferment for 3 days.

[0040] 3. The fermented product ...

Embodiment 3

[0045] A method for extracting and preparing selenium polysaccharides with high antioxidant activity from selenium-enriched passion fruit peel, comprising the following steps:

[0046] 1. Selenium-enriched passion fruit, cut in half with a knife, dig out the pulp containing seeds with a pulper (or by hand), wash the remaining passion fruit skin, dry it in a constant temperature drying oven at 45°C, and crush it with a Chinese herbal medicine grinder Pass through a 60-mesh sieve to obtain the selenium-enriched passion fruit peel powder.

[0047] 2. Selenium-enriched passion fruit peel powder 60g, sodium carboxymethylcellulose (CMC-Na) 1.0g, NaNO 3 0.6g, K 2 HPO 4 0.2g, KCl 0.1g, pure water 200ml, according to the access to Bacillus subtilis, according to the 2% inoculation amount, access to the activated mixed seed solution, placed in a constant temperature shaking incubator, set the speed of 150r / min, temperature 35 ℃, ferment for 3 days.

[0048] 3. The fermented product ...

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Abstract

The invention discloses a method for extracting and preparing selenium polysaccharide with high antioxidant activity from selenium-rich passion fruit peel, which comprises the following steps of: pretreating selenium-rich passion fruit peel to obtain selenium-rich passion fruit peel powder, adding a culture solution, inoculating a fermentation strain for fermentation, centrifugally filtering to obtain a precipitate, adding purified water into the precipitate, and homogenizing by using a colloid mill; sieving the homogenized material, heating, and treating by using an ultrahigh-pressure micro-jet homogenizer; and after protein is removed by filtering, adsorbing and drying to obtain passion fruit peel selenium polysaccharide. According to the method, the selenium polysaccharide in the passion fruit peel can be rapidly extracted and utilized, and compared with a traditional ultrasonic-assisted hot water extraction method, the extraction recovery rate is high, and the extracted selenium polysaccharide is good in quality, high in oxidation resistance and hypoglycemic activity and remarkable in economic value effect.

Description

technical field [0001] The invention relates to the technical field of food processing, in particular to a method for extracting and preparing selenium polysaccharides with high antioxidant activity from selenium-enriched passion fruit peel. Background technique [0002] Passion fruit is a herbaceous vine of the Passiflora family Passiflora, and the fruit can be eaten raw or used as a vegetable. Being used as medicine has the effect of excitement and strength. The fruit pulp is juicy, which can be made into an aromatic and delicious drink, and can also be added to other drinks to improve the quality of the drink. The seeds are pressed for oil, which can be used for food, soap making, paint making and so on. Passion fruit contains 17 kinds of amino acids, rich in protein, fat, sugar, vitamins, calcium, phosphorus, iron, potassium and other substances beneficial to the human body. It is also known as the king of VC (vitamins) in fruits because of its unique taste , sweet an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
CPCC08B37/0003
Inventor 游向荣周葵李明娟王颖张雅媛卫萍
Owner GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI
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