Human lung blood-air barrier simulation device and simulation analysis method for influence of air composition on human lung

Abstract

The invention belongs to the technical field of antigen detection, and particularly relates to a human lung blood-air barrier simulation device which comprises a pathway layer and a barrier layer; the pathway layer comprises an exchange cavity, an exchange channel and an exchange hole, the exchange cavity is a groove formed in the first surface of the pathway layer, the exchange hole is formed in the second surface of the pathway layer, and the exchange hole is communicated with the exchange cavity through the exchange channel; the barrier layer comprises a skeleton layer, a first simulation layer and a second simulation layer; the first simulation layer is an A549 cell layer proliferated on the first surface of the skeleton layer; the second simulation layer is an HUVEC cell layer proliferated on the second surface of the skeleton layer; the pathway layers are arranged on the two faces of the barrier layer respectively, and the first faces of the pathway layers are close to the barrier layer. The human lung blood-air barrier simulation device has beneficial effects are that the simulation device is simple in structure and low in cost; the A549 cell layer and the HUVEC cell layer are respectively arranged, so that the structure of pulmonary alveolar capillary vessels is effectively simulated; rhythmic stretching of the vacuum pump is used for simulating contraction and relaxation of pulmonary alveolar caused by human respiration; the simulation device is simple to operate; the exchange cavity is in a streamline spindle shape, and fluid flowing non-uniformity is prevented by considering fluid mechanics factors.

Description

technical field [0001] The invention belongs to the technical field of antigen detection, and in particular relates to a human lung air-blood barrier simulation device and a simulation analysis method for the influence of air components on human lungs. Background technique [0002] At present, with the rapid development of science and technology and economy in our country, the problem of air pollution is very serious and has attracted widespread attention. [0003] In the process of studying the impact of air pollutants on human lungs, it has been found that air pollutants such as PM2.5 are related to the occurrence and development of various lung diseases, such as lung inflammation, asthma, chronic obstructive pulmonary disease, and even lung cancer. At present, the mechanism of pollution-induced lung damage mainly includes oxidative stress, inflammatory response and DNA damage, etc., but there is no unified conclusion yet. Therefore, further elucidation of the lung toxicit...

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Problems solved by technology

[0005] Although animal models consider the impact of air pollutants on the body as a whole, they are costly, time-consuming, and difficult to conduct quantitative research in vivo; in addition, due to differences between species, animal models cannot represent human physiological and pathological characteristics to some extent , causing limitations in the application of animal models

[0006] In vitro cell models use cultured human lung cell lines for quantitative and high-throughput analysis. However, due to the inability to realize the in vivo cell environment, the lack of information interaction and mechanical force between various cells, it is difficult to simulate the results in vivo. The actual situation

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