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Denitrifying bacterium pseudomonas sp. JNB12 and application thereof

A technology of Pseudomonas and Pseudomonas preparations, applied in the field of environmental microorganisms

Active Publication Date: 2021-09-10
RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in recent years, aerobic denitrifying bacteria have been discovered, which overcomes the shortcomings of traditional denitrifying bacteria that can only denitrify under anoxic conditions.

Method used

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  • Denitrifying bacterium pseudomonas sp. JNB12 and application thereof
  • Denitrifying bacterium pseudomonas sp. JNB12 and application thereof
  • Denitrifying bacterium pseudomonas sp. JNB12 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Embodiment 1, material preparation:

[0034] A. BTB isolation medium:

[0035]

[0036] B. Denitrification Medium (DM):

[0037]

[0038] Among them, trace elements are: (ultrapure water 1L)

[0039] CuSO 4 ·5H 2 O 0.2g ZnSO 4 ·7H 2 O 0.2g

[0040] FeSO 4 ·7H 2 O 0.2g

[0041] C. Beef extract peptone medium

[0042] Beef Extract 3g Peptone 10g

[0043] NaCl 5g pH: 7.0±0.2

[0044] Agar: 1.5% (m / v) ultrapure water 1L

[0045] After the medium was prepared, the pH was adjusted to 7.0 with NaOH, and the plate was prepared after sterilizing with damp heat at 115°C for 30 minutes. Prepare the corresponding liquid culture medium with the above ingredients without adding agar.

Embodiment 2

[0046] Example 2. Bacteria Isolation

[0047] Dilute 20g of soil (for example, red soil in Zhaoqing, Guangdong) in sterile distilled water with a soil-to-water ratio of 1:10 for 0-15cm of surface soil, mix well, take 1ml to 9ml of sterile water for 10-fold serial dilution, and then take 0.2 ml of the suspension was spread on the above-mentioned denitrification culture plate, and cultured at 30°C for 2 weeks. Select a petri dish with 30-100 colonies to pick a single colony with different colony characteristics, separate and culture by streaking, and the culture conditions are the same as above.

Embodiment 3

[0048] Example 3. Screening of bacterial strains with denitrification activity

[0049] A.BTB (bromothymol blue) qualitative detection:

[0050] Since the denitrification reaction is an alkali-producing process (consuming H in the environment + ), when a denitrification reaction occurs, the indicator bromothymol blue of the BTB medium will appear blue, that is, the colony will appear blue or a blue halo. The screened single colony was streaked on a BTB medium plate and cultured at a constant temperature of 30°C for 3 days. When a denitrification reaction occurs, the indicator bromothymol blue of the BTB medium will appear blue, that is, the colony will appear blue or a blue halo. Pick out a single colony with a blue halo from the BTB medium plate, separate and culture by streaking, and culture conditions are the same as above.

[0051] B. Quantitative Screening of Denitrification Medium

[0052] Use the inoculation loop to scrape the single colonies of the two positive strai...

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Abstract

The invention provides a pseudomonas sp. JNB12 with the preservation number of CGMCC No.21605 and application thereof. The pseudomonas sp. JNB12 in the invention can effectively degrade nitrogen in the environment, almost has no accumulation of ammonium nitrogen and nitrite, and does not generate N2O.

Description

technical field [0001] The invention relates to the field of environmental microorganisms. Specifically, the present invention relates to a strain material for assimilating and reducing nitrate, that is, Pseudomonas strain JNB12 (Pseudomonas sp. JNB12), which is preserved by the General Microorganism Center (CGMCC) of the China Microbiological Culture Collection Management Committee, and the preservation number is CGMCC No. 21605. The invention also relates to its use. Background technique [0002] my country is a large agricultural country. In order to increase production, a large amount of chemical nitrogen fertilizers are used in the agricultural production process, a considerable part of which is not absorbed by crops and accumulates in the soil in the form of nitrate. On the one hand, the residual nitrate in the soil enters the water body in the form of surface runoff, changing the ecological balance of rivers and lakes, thereby causing algal blooms and eutrophication...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34B09C1/10C02F101/16C12R1/38
CPCC12N1/20C02F3/34B09C1/10C02F2101/163C02F2101/166
Inventor 张丽梅张雅博葛安辉刘四义王琳常曌
Owner RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
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