Fructus gardeniae substitutional tea and method for organoleptic optimization of formula of fructus gardeniae substitutional tea
A technology for substituting tea and gardenia, which can be used in medical formulas, medical preparations containing active ingredients, tea substitutes, etc., and can solve the problems of no matching other effects, no medicinal effects, etc.
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Embodiment 1
[0031] The component determination of embodiment 1 gardenia composition
[0032] Weigh 10g each of gardenia, hawthorn, honeysuckle, kudzu root, imperina root, corn silk, mulberry leaf, poria cocos, hangchrysanthemum, licorice, tangerine peel, and dried ginger into a round bottom flask, add 10 times the amount of distilled water, and extract for 1 hour. Use a rotary evaporator to rotate and concentrate to 0.5g / mL, and store in a 4°C refrigerator for later use.
[0033] Xanthine oxidase activity assay
[0034] Total reaction volume 5mL, sample solution 5mg / mL, enzyme solution 0.02U / mL, 3mL buffer and 1mL 1.5mmol / mL substrate solution, xanthine generates uric acid under the catalysis of xanthine oxidase, at 295nm There are characteristic absorption peaks, which are detected once every 30 minutes and once every 1 hour. In the blank group, an equal amount of buffer solution was used instead of the enzyme solution.
[0035] Inhibition rate Calculate the percentage of inhibition of...
Embodiment 2
[0042] Embodiment 2 gardenia jasmine substitute tea technological step
[0043] (1) Selection of raw materials: positive color, no mildew, no impurities, no insect bites, meeting the basic requirements for substitute tea raw materials.
[0044] (2) Pre-treatment: After pulverizing gardenia, hawthorn, kudzu root, cocos root, poria cocos, licorice, tangerine peel, and dried ginger with a traditional Chinese medicine grinder, pass through a 10-mesh sieve. Repeat the crushing of the unsifted surface until all are sieved.
[0045] (3) Extraction of geniposide: use gardenia jasminoides for extraction and purification of geniposide, concentrate in vacuum and freeze-dry to obtain geniposide extract.
[0046] (4) Mixing: According to the formula in Example 1, each raw material is mixed evenly.
[0047] (5) Packaging: meet the corresponding hygienic requirements, should be clean, dry, odorless, non-toxic, and should have good airtightness.
Embodiment 3
[0048] Example 3 Gardenia substitute tea formula optimization
[0049] On the basis of the single factor experiment, the Box-Benhken experimental design was adopted to set the addition amount of tangerine peel (A), licorice addition amount (B), hawthorn addition amount (C) and gardenia composition (gardeniposide added) addition amount (D) 4 factors are independent variables, with the single factor result as the center point, 3 levels are set, and the sensory score (Y) is used as the response value to optimize the formula of gardenia substitute tea. The factors and levels of the response surface test are shown in Table 2 .
[0050] Table 2 Response surface test factor levels
[0051]
[0052]
[0053] Product sensory evaluation standard
[0054] Weigh 2g / bag of tea sample, add 200mL boiling water to brew for 20min, smell its aroma, distinguish its taste, and check whether there is any foreign matter at the bottom of the beaker. The evaluation is carried out according t...
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