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Preparation method of hydrophobic and lipophilic nanocellulose based on immobilized enzyme chemistry-oxidative degumming

A technology of immobilized enzymes and chemical degumming, which is applied in the direction of producing bast fibers by chemical methods, can solve the problems of non-repeatability, polluting enzymes, and large aspect ratio, so as to reduce production costs, improve separation efficiency, and increase regulation. Effect

Active Publication Date: 2022-08-02
基准(北京)生物技术研究院有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The nanocellulose prepared by the method of the present invention has a diameter of 4-12nm, a large aspect ratio, and has intrinsic hydrophobicity and lipophilicity without modification, which solves the problem of hydrophilic and lipophilic nanocellulose prepared in the prior art. Chemical modification or surface finishing can improve its lipophilicity and realize its dispersion in hydrophobic polymers, and the preparation method of the present invention adopts a new immobilized enzyme-chemical degumming agent, which solves the problem of existing nanofibers A large amount of acid or alkali is consumed in the preparation process of cellulose, which leads to serious pollution problems and the problem of unrepeatable use of enzymes in the existing nanocellulose biological method

Method used

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  • Preparation method of hydrophobic and lipophilic nanocellulose based on immobilized enzyme chemistry-oxidative degumming
  • Preparation method of hydrophobic and lipophilic nanocellulose based on immobilized enzyme chemistry-oxidative degumming
  • Preparation method of hydrophobic and lipophilic nanocellulose based on immobilized enzyme chemistry-oxidative degumming

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Add 4 g of sodium alginate to 200 g of room temperature water, heat up to 80°C, dissolve for 90 min to obtain a sodium alginate solution; add 10 g of pectinase to the above solution, stir and disperse at room temperature for 120 min, after centrifugal defoaming, add a syringe, use peristaltic motion The pump is extruded through a 20G needle, and after passing through an air curtain of 8cm, the stirring speed is 300rpm, and the mass fraction is 6% calcium chloride solution. After gelation for 20min, the immobilized enzyme microspheres (such as Figure 7 Rinse 4 to 5 times with Tris-HCl (8%, 8g / 1000mL) solution, dehydrate, pour into petri dishes, freeze at 14~-18℃ for 24h, then freeze-dry at -55℃ for 72h, The immobilized pectinase with microporous structure was obtained.

Embodiment 2

[0048] Take 100g of distilled water, adjust the pH to 8 with sodium hydroxide, add 0.4g of krill protein, stir to dissolve, add 1.6g of sodium alginate after the protein is dissolved, heat up to 60°C, and continue to dissolve for 90min to obtain sodium alginate / krill Protein solution; add 12g of pectinase to the above solution, stir and disperse at room temperature for 120min, after centrifugal defoaming, add a syringe, extrude it through a 20G needle with a peristaltic pump, go through an 8cm air curtain, enter the stirring speed of 300rpm, mass fraction is 6% calcium chloride solution, after gelation for 20min, the immobilized enzyme microspheres (such as Figure 8 Rinse 4 to 5 times with Tris-HCl (8%, 8g / 1000mL) solution, dehydrate and pour into a petri dish, freeze at 14~-18°C for 24h, then freeze-dry at -55°C for 72h, The immobilized pectinase with microporous structure was obtained.

Embodiment 3

[0050] Take 100g of distilled water, adjust the pH to 8 with sodium hydroxide, add 0.2g of krill protein, stir to dissolve, add 1.8g of sodium alginate after the protein is dissolved, heat up to 60°C, and continue to dissolve for 90min to obtain sodium alginate / krill Protein solution; add 13g pectinase to the above solution, stir and disperse at room temperature for 120min, after centrifugal defoaming, add a syringe, extrude through a 20G needle with a peristaltic pump, and pass through an air curtain of 8cm, enter the stirring speed of 300rpm, mass fraction It is 6% calcium chloride solution, after gelation for 20min, the immobilized enzyme microspheres are separated by filtration; rinsed 4 to 5 times with Tris-HCl (8%, 8g / 1000mL) solution, dehydrated and poured into a petri dish, Freeze at 14~-18°C for 24h, and then freeze-dry at -55°C for 72h to obtain immobilized pectinase with microporous structure.

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Abstract

The invention discloses a green intrinsic hydrophobic and lipophilic nanocellulose and a preparation method thereof. In the present invention, hemp rods are used as raw materials, and self-developed immobilized enzyme-chemical degumming agent and oxidative degumming agent are used as cooking media, and nanocellulose with intrinsic hydrophobic and lipophilic properties can be prepared efficiently and greenly through alternate cooking of the two. The nanocellulose prepared by the method of the invention has a diameter of 4-12 nm, a large aspect ratio, and has inherent hydrophobicity and lipophilicity without modification. Modification to improve its lipophilicity to achieve the problem of its dispersion in the hydrophobic polymer, and the present invention solves the existing nanocellulose preparation process well by adopting a new immobilized enzyme-chemical degumming agent with low salt content A large amount of acid or alkali is consumed, causing serious pollution problems.

Description

technical field [0001] The invention relates to a nanocellulose and a preparation method thereof, in particular to a green intrinsically clean high-efficiency hydrophobic and lipophilic nanocellulose and a preparation method thereof. Background technique [0002] my country is a big country in the cultivation of hemp plants, and its output ranks first in the world, but the current application is concentrated in its bast, seeds and other parts, and the remaining large amount of hemp stems are usually discarded or incinerated as agricultural waste, which not only wastes resources, but also also caused environmental pollution. Recycling hemp rods not only has environmental benefits, but also reduces the economic cost of the hemp fiber industry to a certain extent, thereby expanding the development of the hemp industry. [0003] Immobilized pectinase, which means that the pectinase can be immobilized on the substrate, has an effect on increasing the reuse of the pectinase and re...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): D01C1/02
CPCD01C1/02
Inventor 张欣郭静季英超贾蕾青
Owner 基准(北京)生物技术研究院有限公司