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Application of AgOTf as reactive matrix in detection of bromine-containing compounds in MALDI mass spectrum

A bromine compound and reactive technology, applied in the field of biochemical analysis of bromine-containing compounds, can solve problems such as limiting the application range of MALDI technology, poor ionization efficiency, and limiting sensitive analysis

Active Publication Date: 2021-09-14
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the ionization efficiency of many bromine-containing organic compounds is poor in MALDI, which largely limits the sensitive analysis of these substances by MALDI technology
In addition, there is currently no MALDI matrix suitable for the analysis of inorganic bromine-containing compounds, which limits the application range of MALDI technology to some extent
It can be seen that the current detection methods for bromine-containing compounds still have deficiencies and need to be improved.

Method used

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  • Application of AgOTf as reactive matrix in detection of bromine-containing compounds in MALDI mass spectrum
  • Application of AgOTf as reactive matrix in detection of bromine-containing compounds in MALDI mass spectrum
  • Application of AgOTf as reactive matrix in detection of bromine-containing compounds in MALDI mass spectrum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] Example 1: Combining SA with a reactive substrate to construct a bromine-containing compound target plate reaction strategy

[0066] (1) Prepare a 10mg / mLSA methanol solution, which can be stored in a refrigerator at 4°C;

[0067] (2) Prepare AgTFA and AgOTf substrates respectively, using methanol as solvent and AgTFA and AgOTf as solutes, respectively prepare 10mg / mL AgTFA and AgOTf solutions, which can be stored in a refrigerator at 4°C;

[0068] (3) Prepare 500μg / mL TBBPA, TBC and TEAB methanol solutions respectively, which can be stored in a refrigerator at 4°C;

[0069] (4) Take 1 μL of TBBPA, TBC and TEAB solution in step (3), 1 μL of AgOTf in step (2), and 1 μL of SA matrix solution in step (1), prepare samples by dry drop method, and take 1 μL in polished Spot the sample on the spotting hole of the steel target plate. Dry naturally at room temperature 25°C;

[0070] (5) As a control, 1 μL of TBBPA, TBC and TEAB solution in step (3), 1 μL of AgTFA in step (2),...

Embodiment 2

[0075] Example 2: Combining AgOTf with a commercial substrate to construct a bromine-containing compound target plate reaction strategy

[0076] (1) Prepare 10mg / mL DHB, CHCA, NDA, NEDC, 3-AQ, DCTB and THAP methanol solutions, which can be stored in a refrigerator at 4°C;

[0077] (2) Take 1 μL of TBBPA, TBC and TEAB solution in Example 1 and 1 μL of AgOTf in Example 1, 1 μL of SA matrix solution in Example 1 or 1 μL of DHB, CHCA, NDA prepared in step (1), NEDC, 3-AQ, DCTB or THAP matrix solution, use the dry drop method to prepare samples, and take 1 μL to spot on the wells of the polished steel target plate. Dry naturally at room temperature 25°C;

[0078] (3) As a control, take 1 μL of TBBPA, TBC and TEAB solution in Example 1 and 1 μL of AgOTf in Example 1, prepare samples by dry drop method, and take 1 μL to spot on the wells of the polished steel target plate. Dry naturally at room temperature 25°C;

[0079] (4) The target plate is sent to the MALDI mass spectrometer,...

Embodiment 3

[0081] Example 3: Qualitative investigation of the ability of AgOTf / SA matrix to detect different bromine-containing compounds

[0082] (1) Prepare 500 μg / mL bronopol, 5,7-dibromo-8-hydroxyquinoline, benzbromarone, bromoindigo, dibromohydantoin, tetrabromobisphenol A bis(2,3- The methanol solution of dibromoallyl) ether, tribromophenol, tris(tribromophenoxy)triazine and lithium bromide can be stored in a refrigerator at 4°C;

[0083] (2) Take 1 μL of AgOTf in Example 1, 1 μL of the SA matrix solution in Example 1 and bronopol, 5,7-dibromo-8-hydroxyquinoline, benzbromarone, and bromide in step 1, respectively. Methanol solution of indigo, dibromohydantoin, tetrabromobisphenol A bis(2,3-dibromoallyl) ether, tribromophenol, tris(tribromophenoxy)triazine, or lithium bromide, prepared by the dry drop method Take a sample, and take 1 μL to spot on the well of the polished steel target plate.

[0084] Dry naturally at room temperature 25°C;

[0085] (3) As a control, take 1 μL of ...

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Abstract

The invention discloses application of a combination (AgOTf / auxiliary matrix) of silver trifluoromethanesulfonate (AgOTf) added as a novel reactive matrix and an auxiliary matrix in high-flux qualitative and quantitative detection of bromine-containing compounds. In the MALDI mass spectrum, a target plate reaction strategy based on an AgOTf / auxiliary matrix can be used to realize high-flux qualitative and quantitative analysis of bromine-containing compounds. AgOTf has a silver group and is easy to react with halogen atoms as a reactive matrix, and a series of compounds [AgBr + Br] <->, [2AgBr + Br] <->, [AgOTf + Br] <-> and [2AgOTf + Br] <-> can be generated. The auxiliary matrix can assist ionization of the generated compounds. The AgOTf / auxiliary matrix is applicable to detection of organic and inorganic bromine-containing compounds as a matrix.

Description

technical field [0001] The invention relates to the field of biochemical analysis of bromine-containing compounds, in particular to the application of AgOTf as a reactive matrix in the detection of bromine-containing compounds in MALDI mass spectrometry. Background technique [0002] Brominated compounds widely exist in the environment and have a significant impact on human health. Bromine is an essential element for the human body and plays an important role in many life processes. Lack or excessive intake of bromine will be detrimental to human health. Bromine-containing compounds in the atmosphere can affect the earth's albedo and global climate, and participate in a variety of atmospheric photochemical reactions, destroying the ozone layer. The bromine element in sewage can react with dissolved organic matter during the disinfection process to form bromine-containing disinfection by-products which are more harmful than chlorine-containing disinfection by-products. In ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/64G01N1/28
CPCG01N27/64G01N1/28
Inventor 潘远江冯鸿儒童艺
Owner ZHEJIANG UNIV
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