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Carbon quantum dot hydrogel composite scaffold material and preparation method and application

A technology of carbon quantum dots and composite scaffolds, applied in the field of biomedical engineering, can solve the problems of not being able to effectively promote osteogenic differentiation, and achieve the effects of promoting osteogenic differentiation, good antibacterial activity and osteogenic activity, and simple preparation process

Active Publication Date: 2022-07-01
SHANGHAI SIXTH PEOPLES HOSPITAL
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to address the deficiencies of the prior art, to provide carbon quantum dot hydrogel composite scaffold material and its preparation method and application, let the hydrogel be used as a scaffold of carbon quantum dots, improve the hydrogel itself can not effectively promote osteogenesis Insufficient differentiation, overcome the defect that carbon quantum dots cannot be used alone in vivo to promote osteogenic differentiation, and at the same time endow scaffold materials with excellent antibacterial activity, and promote the healing of infectious bone defects in vivo as soon as possible

Method used

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  • Carbon quantum dot hydrogel composite scaffold material and preparation method and application
  • Carbon quantum dot hydrogel composite scaffold material and preparation method and application
  • Carbon quantum dot hydrogel composite scaffold material and preparation method and application

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Experimental program
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Embodiment 1

[0043] see figure 2 , a carbon quantum dot hydrogel composite scaffold material, including p-CQD / WS2 heterojunction with antibacterial activity, n-CQD with osteogenic differentiation activity, p-CQD / WS2 / n composed of photosensitizer and GelMA -CQD / GelMA hydrogel scaffold material.

[0044] As a further supplement of this embodiment, the p-CQD / WS2 heterojunction is an electrically neutral carbon quantum dot / tungsten sulfide nanosheet heterojunction.

[0045] As a further supplement to this embodiment, the n-CQDs are negatively charged functionalized carbon quantum dots.

[0046] As a further supplement to this example, the photosensitizer is Irgacure 2959. Irgacure 2959 is a potentiated non-yellowing UV photoinitiator.

[0047] As a further supplement to this embodiment, the GelMA is a methacrylated hydrogel.

Embodiment 2

[0049] see figure 2 , a preparation method of a carbon quantum dot hydrogel composite scaffold material, comprising the following steps: firstly taking the freeze-dried solid of GelMA, adding polybutylene succinate, p-CQD / WS2 heterojunction and n-CQD, and finally adding The photosensitizer is continuously irradiated under ultraviolet light until the hydrogel is solidified, and the p-CQD / WS2 / n-CQD / GelMA hydrogel scaffold material is obtained.

[0050] As a further supplement of this example, the preparation process of the freeze-dried solid of GelMA is as follows: first take 0.5-1.5g of gelatin and preheat at 45-55°C for 8-12min, then add 6-10mL of methacrylic acid and vigorously stir; Then preheat polybutylene succinate at 35-45°C for 8-12min, then take 30-50mL polybutylene succinate and add it to the mixed solution formed by gelatin and methacrylic acid; After completion, the reacted mixture was dialyzed at 40-50° C. for 66-78 h, and finally freeze-dried with a freeze dryer...

Embodiment 3

[0055] To prepare positively charged carbon quantum dots (p-CQDs) with antibacterial activity, the specific implementation process is as follows:

[0056] (a) Weigh 0.1g of spermidine and dissolve it in 10mL of deionized water, ultrasonically treat for 10min to mix the spermidine solution evenly, transfer the spermidine solution to a special reaction glass tube for a microwave reactor, and the microwave reaction conditions are 200 °C, and the reaction time was 3 min.

[0057] (b) After the reaction is completed, after naturally cooling to room temperature, the obtained reaction product is filtered through a 0.22 μm filter membrane to remove the unreacted reactant, and dialyzed using a 1000Da dialysis bag for two days.

[0058] Depend on Figure 1-a , Figure 1-b , Figure 1-c and Figure 1-d It can be seen that the size of the p-CQDs with antibacterial activity is 4.7±1.1 nm, and the interlayer spacing is 0.376 nm.

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Abstract

The invention belongs to the field of biomedical engineering, and in particular relates to a carbon quantum dot hydrogel composite scaffold material and a preparation method and application. The scaffold material includes p-CQD / WS2 heterojunction with antibacterial activity, n-CQD with osteogenic differentiation activity, a p-CQD / WS2 / n-CQD / GelMA hydrogel scaffold material composed of a photosensitizer and GelMA. In the present invention, a 3D composite scaffold material is formed by jointly loading the carbon quantum dots with antibacterial activity and the carbon quantum dots with the activity of promoting osteogenic differentiation on the hydrogel, so that the hydrogel can be used as a scaffold for the carbon quantum dots. Improve the deficiency that the hydrogel itself cannot effectively promote osteogenic differentiation, overcome the defect that carbon quantum dots cannot be used alone to promote osteogenic differentiation in vivo, and at the same time endow the scaffold material with excellent antibacterial activity to promote the in vivo infection of bone defects. Heal as soon as possible.

Description

technical field [0001] The invention relates to the field of biomedical engineering, in particular to a carbon quantum dot hydrogel composite scaffold material and a preparation method and application. Background technique [0002] As an important tissue organ of the human body, bone tissue has important functions in the body, such as movement, support and protection of tissue structure, hematopoiesis, and storage of mineral ions. The metabolism of bone tissue is very active, and physiological bone remodeling accompanies human life, so bone tissue has the ability of self-renewal and self-repair. However, with the increase of age, the self-repair ability of bone will weaken; if the defect caused by bone injury is larger, its self-repair will also be limited, and the less completely repaired and severe bone trauma may even cause infection. [0003] The clinical treatment of infected bone defects is a formidable challenge. The traditional method of treating infected bone defe...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/52A61L27/54A61L27/50A61L27/08A61L27/02A61L27/22
CPCA61L27/52A61L27/54A61L27/50A61L27/08A61L27/025A61L27/222A61L2430/02A61L2300/108A61L2300/404A61L2300/412
Inventor 沈龙祥耿弼江潘登余
Owner SHANGHAI SIXTH PEOPLES HOSPITAL
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