Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for identifying bacterial infection type based on duckweed

A duckweed and bacteria technology, applied in material excitation analysis, fluorescence/phosphorescence, instruments, etc., can solve the problems of insensitive detection results, cumbersome processing steps, and many chemical reagents, so as to achieve less use of reagents and promote whether to cure diseases. , the effect of sensitive identification

Pending Publication Date: 2021-09-17
GUANGDONG ACAD OF SCI INST OF BIOENGINEERING
View PDF5 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the current method of studying the type of plant tissue infected by bacteria, there are many chemical reagents, the preparation is complicated, and it takes at least 20 minutes to sterilize the plant tissue. The processing steps are cumbersome, time-consuming, not fast enough, and the detection results are not sensitive enough.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for identifying bacterial infection type based on duckweed
  • Method for identifying bacterial infection type based on duckweed
  • Method for identifying bacterial infection type based on duckweed

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Embodiment 1 A kind of method based on duckweed identification bacterial infection type

[0036]A kind of method of identifying bacterial infection based on duckweed of the present embodiment comprises the following steps:

[0037] 1. Construction of sterile duckweed system

[0038] Wash the collected duckweed in sterile water for 3 to 5 times, put 5 to 10 pieces of duckweed in a 10 mL sterile centrifuge tube, add 5 mL of 50% alcohol to wash for 10 min, and discard the supernatant. Add 5mL of 5% sodium hypochlorite to wash for 2min, then rinse with sterile water 2-3 times.

[0039] Duckweed was transferred to plant medium (1 / 2 SH + 0.5% sucrose + 0.1% cefotaxime), where SH is the abbreviation for Schenk & Hildebrandt Basal Salt Mixture plant medium. After the duckweed grows on the culture medium for 7 days, place the duckweed on LB (beef extract peptone) bacterial culture medium and observe overnight in an incubator at 28°C. If there are no colonies around the duckw...

Embodiment 2

[0078] Embodiment 2 A kind of method based on duckweed identification bacterial infection type

[0079] A kind of method of identifying bacterial infection based on duckweed of the present embodiment, except that the concentration of each component of the chemical solution (CM) of the present invention is different from that of Example 1 in step 3b, others are all the same as Example 1; this implementation The chemical solution of the example is composed of the following components: 135mM NaCl, volume concentration 40% alcohol, 5% (g / 100ml) NaClO, pH7.6. The result of the method for identifying the type of bacterial infection based on duckweed in this example is consistent with Example 1.

Embodiment 3

[0080] Embodiment 3 A kind of method based on duckweed identification bacterial infection type

[0081] A kind of method of identifying bacterial infection based on duckweed of the present embodiment, except that the concentration of each component of the chemical solution (CM) of the present invention is different from that of Example 1 in step 3b, others are all the same as Example 1; this implementation The chemical solution of the example is composed of the following components: 140mM NaCl, volume concentration 35% alcohol, 4% (g / 100ml) NaClO, pH7.8. The result of the method for identifying the type of bacterial infection based on duckweed in this example is consistent with Example 1.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for identifying bacterial infection types based on duckweed. The method comprises the following steps: co-culturing to-be-detected bacteria and sterile duckweed in a 1 / 2SH culture solution to obtain inoculated duckweed; cleaning the inoculated duckweed; and dyeing the duckweed by adopting a green fluorescent nucleic acid dye DMAO. According to the method for identifying the bacterial infection type based on the duckweed, on the basis of a functional research system of an aquatic model plant of the duckweed, a chemical solution with special components and concentrations is adopted to clean the leaf tissues of the duckweed, and then a green fluorescent nucleic acid dye DMAO is combined for dyeing, so that the type of the bacterial infection tissue is rapidly identified, and related researches for judging whether infection bacteria treat host plants, whether a symbiotic relationship is established between microorganisms and plants, how to interact between the microorganisms and the plants and the like can be promoted.

Description

technical field [0001] The invention belongs to the technical field of bacteria function research, and more specifically, the invention relates to a method for identifying bacteria-infected tissue types based on duckweed. Background technique [0002] After the bacteria in the natural environment enter the plant body, due to physiological adaptation and other factors, some bacteria can survive in the plant body for a long time, and can spread with seeds or asexual reproduction materials between generations of plants, and become the source of endophytic bacteria in offspring plants. main source. However, some bacteria attach to the surface of plant tissues and play a positive role in promoting the growth and physiology of plants or causing diseases. [0003] Duckweed is a small floating water plant, due to its small genome, simple structure, less repetitive sequences, vegetative reproduction, short genetic cycle (about 30h for one generation under good nutritional conditions...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N21/64
CPCG01N21/6428G01N21/6458
Inventor 孙东磊黄卫娟付建涛安玉兴卢颖林
Owner GUANGDONG ACAD OF SCI INST OF BIOENGINEERING
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com