In-vitro biological activity determination method of leech medicinal materials, decoction pieces and processed products
A technology of biological activity and measurement method, applied in the field of in vitro biological activity measurement, can solve the problems of weak correlation, undetectable antithrombin activity, weakened anticoagulant activity, etc.
Pending Publication Date: 2021-09-17
山东禹泽药康产业技术研究院有限公司 +1
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Abstract
The invention relates to an in-vitro biological activity determination method for leech medicinal materials, decoction pieces and processed products, which are characterized in that after leeches are subjected to protease enzymolysis by pepsin and trypsin, thrombin titration is carried out to determine the antithrombin activity. In-vitro activity detection can be carried out on leech medicinal materials, decoction pieces and processed products, and the in-vitro activity detection result is basically consistent with an in-vivo drug effect conclusion.
Application Domain
Chemical analysis using titrationBlood disorder +2
Technology Topic
Medicinal herbsProtease +9
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PUM
Property | Measurement | Unit |
Vitality | 2500.0 | U/mg |
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