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Application of glucogalactooligosaccharide in preparation of medicine for treating and/or preventing ischemia-reperfusion injury

A galacto-oligosaccharide, ischemia-reperfusion technology, applied in drug combinations, pharmaceutical formulations, cardiovascular system diseases, etc., to reduce cognitive dysfunction, improve neurological deficits, and alleviate liver ischemia-reperfusion injury. Effect

Active Publication Date: 2022-03-18
NANJING SOUTHERN ELEMENT BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, whether the galactooligosaccharide has a protective effect on ischemia-reperfusion injury has not been reported yet.

Method used

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  • Application of glucogalactooligosaccharide in preparation of medicine for treating and/or preventing ischemia-reperfusion injury
  • Application of glucogalactooligosaccharide in preparation of medicine for treating and/or preventing ischemia-reperfusion injury
  • Application of glucogalactooligosaccharide in preparation of medicine for treating and/or preventing ischemia-reperfusion injury

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Protective effect of glucogalactooligosaccharides on hepatic ischemia-reperfusion injury

[0042] 1. Establishment of animal models

[0043] The experimental animals are male C57 / BL6J mice, 6-8 weeks old, 22-26g. Raised under standard experimental conditions, 12-hour light-12-hour dark cycle, free intake of water and food. The mice were anesthetized with isoflurane, and the abdomen was operated on, and the blood supply of the middle lobe and left lobe of the liver was blocked with a non-traumatic hemostatic clip, resulting in 70% liver ischemia. After 30 min of ischemia, the clip was removed, and then reperfused After 6 hours, samples were taken to measure biochemical indicators.

[0044] 2. Experimental grouping

[0045]By intraperitoneal injection, the mice were randomly divided into four groups, the first group was the sham operation group (sham), the animals in the sham operation group received the same laparotomy, but no vascular clip was blocked; the second gro...

Embodiment 2

[0056] Protective effect of glucogalactooligosaccharides on renal ischemia-reperfusion injury

[0057] 1. Establishment of animal models

[0058] The experimental animals were male C57 / BL6J mice, 6-8 weeks old, 22-26g. The mice were anesthetized with isoflurane, and the abdomen was operated on. Under sterile conditions, a 1.5 cm longitudinal incision was made along the double dorsal side to enter the abdominal cavity, the muscles were bluntly separated, and the renal pedicles on both sides were exposed. After 30 minutes of ischemia, the arterial clamp was released, and the abdominal cavity was closed after the renal perfusion resumed. After 24 hours, samples were taken to measure biochemical indicators, so as to establish the RIRI model.

[0059] 2. Experimental grouping

[0060] By intraperitoneal injection, the mice were randomly divided into four groups, the first group was the sham operation group (sham), the animals in the sham operation group received the same laparoto...

Embodiment 3

[0071] The protective effects of glucogalactooligosaccharides against cerebral ischemia-reperfusion injury.

[0072] 1. Establishment of animal models

[0073] The experimental animals are male C57 / BL6J mice, 6-8 weeks old, 22-26g. Anesthetize mice with isoflurane and place them supine on a heating pad. The skin was disinfected with iodine and 75% ethanol. A midline neck incision was made to expose the carotid artery (MCA). The external carotid artery (ECA) was then isolated and occluded with two knots. Next, the internal carotid artery (ICA) was isolated, and the carotid artery (MCA) and internal carotid artery (ICA) were clamped with microvascular clamps. A small hole was cut in the external carotid artery (ECA) between the junction and the bifurcation. Then, the MCAO suture with a length of 30 mm and a diameter of 0.11 mm coated with silicone rubber was introduced into the internal carotid artery (ICA). While sutures are inserted to block the blood vessel, the artery ...

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Abstract

The invention discloses an application of glucogalactooligosaccharide in the preparation of medicines for treating and / or preventing ischemia-reperfusion injury. The galactooligosaccharides described are: the galactooligosaccharides of the present invention can significantly reduce postoperative liver injury, kidney injury, and brain injury in ischemia-reperfusion mice, and are safe, non-toxic, and have strong drug effects. , has good medicinal prospects in preventing and treating ischemia-reperfusion injury.

Description

technical field [0001] The invention belongs to the technical field of drugs for ischemia-reperfusion injury, and relates to the application of glucogalactooligosaccharide in the preparation of drugs for treating and / or preventing ischemia-reperfusion injury. Background technique [0002] Ischemia-reperfusion injury (IRI) refers to the phenomenon that tissue and organ ischemia and reperfusion cannot restore its function, but aggravate the dysfunction and structural damage of tissues and organs. The pathogenesis of ischemia-reperfusion involves multiple mechanisms, including activation of neutrophils, oxidative stress, ATP and glycogen depletion, and cellular metabolic stress due to mitochondrial dysfunction. The mechanism of this pathophysiological process is complex, and there is still a lack of comprehensive and effective treatment methods and specific drugs for ischemia-reperfusion injury clinically. The liver, kidney, and brain are common organs that are injured by isch...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/715A61P9/10
CPCA61K31/715A61P9/10A61K9/0019A61K47/02A61K47/26
Inventor 张建法王妮赵扬
Owner NANJING SOUTHERN ELEMENT BIOTECHNOLOGY CO LTD
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