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Identification device and method for tyrophagus putrescentiae resistance of pleurotus ostreatus

An identification method and rotting technology, applied in the fields of botanical equipment and methods, mushroom cultivation, horticulture, etc., to achieve the effect of ensuring repeatability and accuracy, ensuring consistency, and shortening the breeding cycle

Active Publication Date: 2021-10-01
JIANGSU ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Germplasm resources with excellent resistance are the material basis for insect-resistant breeding. However, there are no evaluation standards for the identification of Pleurotus ostreatus and reports of related resistance identification at home and abroad so far. The resource identification method is of great significance for the screening of anti-mite germplasm resources of Pleurotus ostreatus and the research of resistance breeding, and is also of great significance for promoting the sustainable control of edible fungus harmful organisms and the sustainable development of agriculture.

Method used

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  • Identification device and method for tyrophagus putrescentiae resistance of pleurotus ostreatus
  • Identification device and method for tyrophagus putrescentiae resistance of pleurotus ostreatus
  • Identification device and method for tyrophagus putrescentiae resistance of pleurotus ostreatus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0073] This example provides an indoor rapid identification method for the resistance of Pleurotus ostreatus to tyrophagous mite.

[0074] 1. Mycelia culture of germplasm resources of Pleurotus ostreatus to be tested

[0075] Mycelium was used in this test analysis.

[0076] Cultivation and preservation of mycelium: Inoculate the parent species of different germplasm resources for testing in PDA medium (petri dish diameter d=70mm), inoculate 3 plates for each variety, and culture in the dark at 25°C after sealing with parafilm , in an incubator with 80% RH in the dark until the hyphae are overgrown, and stored in a refrigerator at 4°C for later use.

[0077] The PDA medium covered with mycelia is punched into uniform bacterium pieces (d=5mm) with a puncher, and placed in the culture cavity 11 in the inner box base 12 (sterilized, and containing 1mL of PDA solid medium) In each well, put a bacterial block, 6 strains as a plate.

[0078] Remember the variety number, cover the...

Embodiment 2

[0086] This example provides field resistance identification of Pleurotus ostreatus against Tyrophagous rot mite.

[0087] 1. Production of fruiting bottles of Pleurotus ostreatus germplasm resources to be tested

[0088] Raw material configuration for Pleurotus ostreatus cultivation: Mix 90% cottonseed hulls and 10% bran, add water, and stir well to make the water content 60% to 65%. Pack the prepared raw materials into 300ml plastic transparent vials with a gas-permeable membrane, and each bottle is filled with 200g (± 10g). Put the cap on the bottle and put it into an autoclave for high temperature and high pressure sterilization for 120 minutes. After the sterilization is complete, take it out and let it cool for later use. Different Pleurotus ostreatus strains were inoculated, each with 20 replicates, cultured in the dark at 25°C, in an incubator with 80% RH in the dark, and the fruiting bottles covered with mycelium were stored in a refrigerator at 4°C for later use. ...

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Abstract

The invention provides an identification device and method for tyrophagus putrescentiae resistance of pleurotus ostreatus. The identification method comprises the following steps: activating mother strains of each to-be-identified population of the pleurotus ostreatus in a culture dish containing a PDA culture medium to obtain a PDA culture medium full of mycelia; preparing a plurality of fungus blocks with uniform size from the PDA culture medium full of mycelia, respectively placing the fungus blocks in a culture cavity in an inner box base, and placing the fungus blocks in an artificial climate box for culturing; placing a plurality of tyrophagus putrescentiae adults into the inner box base, and enabling the tyrophagus putrescentiae adults to freely eat; placing the inner box base into an outer box base, and placing the outer box base into the artificial climate box for inoculation of the tyrophagus putrescentiae; and identifying the resistance grade of the pleurotus ostreatus to the tyrophagus putrescentiae. The identification method for tyrophagus putrescentiae resistance of the pleurotus ostreatus can be used for cultivating and identifying anti-mite varieties required by edible mushrooms and ecological environment management, and plays an important role in the field of anti-mite breeding of the edible mushrooms.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to an identification device and an identification method for Pleurotus ostreatus anti-corrosion tyrophagous mite. Background technique [0002] Edible fungus is an important economic crop in my country. With the rapid development of anti-seasonal annual cultivation and three-dimensional high-efficiency cultivation mode of edible fungi, the fungus-eating mites that love temperature and humidity gradually become the main pests from the minor pests that harm edible fungi. [0003] Among them, edible fungus mites have the characteristics of a wide range of hosts, hidden life, and strong environmental adaptability, and have strong drug resistance. Production brings huge economic losses and food safety hazards, making edible fungus mites a destructive pest in the production of edible fungi. [0004] According to current statistics, there are 44 species of mites from 21 families that are harm...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G18/00A01G18/40A01K67/033
CPCA01G18/00A01G18/40A01K67/033
Inventor 李辉平曲绍轩马林骆昕侯立娟蒋宁林金盛徐平
Owner JIANGSU ACAD OF AGRI SCI