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Preparation method of mesenchymal stem cell paracrine factor and application of paracrine factor

A kind of mesenchymal stem cell and paracrine technology, which is applied to the preparation method of peptides, medical preparations containing active ingredients, animal cells, etc., can solve the problems of low efficiency, low content of paracrine factors, low concentration of paracrine factors, etc., to achieve Improve the content, easy and convenient extraction, and assist the effect of fat transplantation

Pending Publication Date: 2021-10-08
中科博赛干细胞再生医学科技广州有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] However, at this stage, the efficiency of isolation, purification, cultivation, doubling, and concentration of paracrine factors in mesenchymal stem cells is low, and it often takes multiple treatments to be effective; at the same time, the concentration of paracrine factors is low, and unpurified and concentrated paracrine factors are obtained. The content in a unit volume of solution is relatively small

Method used

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  • Preparation method of mesenchymal stem cell paracrine factor and application of paracrine factor
  • Preparation method of mesenchymal stem cell paracrine factor and application of paracrine factor
  • Preparation method of mesenchymal stem cell paracrine factor and application of paracrine factor

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Effect test

Embodiment 1

[0046] Embodiment 1: A kind of preparation method of mesenchymal stem cell paracrine factor, it is made by following steps:

[0047] (1) Adipose-derived mesenchymal stem cells were inoculated in α-MEM medium containing 10% fetal bovine serum, and the cell concentration was adjusted to 1×10 7 L -1 placed in 5% CO 2 , the humidity is 90%, the temperature is 37 ° C incubator culture, wash and change the medium once every 3 days, digest and passage with 0.2% trypsin, the obtained mesenchymal stem cell density is greater than 90%, and obtain the required number of mesenchymal stem cells ;

[0048] (2) Inoculate the mesenchymal stem cells in the induction medium, and add an aqueous solution of metformin with a concentration of 400 μmol / L to the induction medium for 24 hours;

[0049] (3) Change the medium in step (2) with serum-free mesenchymal cell culture medium. The medium in this step is Youkang NC0103 medium, and perform starvation treatment for 24 hours according to the fac...

Embodiment 2

[0061] Embodiment 2: The difference from Embodiment 1 lies in step (2):

[0062] (2) Inoculate the mesenchymal stem cells in the induction medium, and add an aqueous solution of metformin with a concentration of 50 μmol / L to the induction medium for 24 hours.

Embodiment 3

[0063] Embodiment 3: The difference from Embodiment 1 lies in step (2):

[0064] (2) Inoculate the mesenchymal stem cells in the induction medium, and add an aqueous solution of metformin with a concentration of 100 μmol / L to the induction medium for 24 hours.

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Abstract

The invention provides a preparation method of a mesenchymal stem cell paracrine factor and application of the paracrine factor, and belongs to the technical field of cosmetics. The preparation method of the mesenchymal stem cell paracrine factor comprises the following steps that (1), mesenchymal stem cells are inoculated into an alpha-MEM culture medium containing 10% of fetal calf serum, the cell concentration is adjusted to be 1*10<7> L<-1>, culturing is carried out in a culture box, digesting and subculturing are carried out by using 0.2% pancreatin, the density of the obtained mesenchymal stem cells is greater than 90%, and the mesenchymal stem cells with a required number are obtained; (2), the mesenchymal stem cells are inoculated into an induction culture medium, a metformin aqueous solution is added into the induction culture medium, and culturing is carried out for 20-30 hours; (3), the serum-free interstitial cell culture medium in the step (2) is replaced, and starvation treatment is carried out; and (4), the cell culture fluid in the step (3) is collected and concentrated, and vacuum freeze-drying is carried out to obtain powder.

Description

technical field [0001] The invention relates to the technical field of cosmetics, in particular to a preparation method of a mesenchymal stem cell paracrine factor and an application of the paracrine factor. Background technique [0002] With the improvement of living standards and the advancement of science and technology, the basic functions such as whitening and moisturizing in the past can no longer meet people's needs for cosmetics, and anti-aging has gradually become an aspect of everyone's concern. In the process of skin aging, active oxygen is an important cause of skin aging, and endogenous aging and exogenous aging act simultaneously. Endogenous aging is mainly due to the gradual accumulation of oxidized and glycosylated proteins with age, resulting in the loss of collagen and elastin in the dermis, thinning of the skin, and degeneration of keratinocytes. Exogenous aging is the deterioration and damage of epidermal tissue due to ultraviolet damage, environmental d...

Claims

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Application Information

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IPC IPC(8): C12N5/0775C07K14/47C07K1/14A61K8/64A61K8/99A61Q19/08
CPCC12N5/0667C12N5/0668C07K14/47A61K8/99A61K8/64A61Q19/08A61K2800/84
Inventor 占国强
Owner 中科博赛干细胞再生医学科技广州有限公司
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