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Super-selenium-rich dunaliella salina product and preparation method and application thereof

A technology of Dunaliella salina and selenium-enriched salts is applied in biochemical equipment and methods, microorganism-based methods, microorganisms, etc., which can solve the problems of undisclosed Dunaliella salina and other problems, and achieve the effect of simple and easy preparation process.

Pending Publication Date: 2021-10-12
SHENZHEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no record in the prior art that Dunaliella salina can be used to combine selenium in selenite and selenate to selenoproteins and selenium-containing amino acids to form organic selenium

Method used

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  • Super-selenium-rich dunaliella salina product and preparation method and application thereof
  • Super-selenium-rich dunaliella salina product and preparation method and application thereof
  • Super-selenium-rich dunaliella salina product and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] A preparation method of super selenium-enriched Dunaliella salina powder, comprising:

[0042] a) Cultivate Dunaliella salina in the culture medium at a temperature of 25±0.5°C and a light intensity of 50 μmol·m -2 the s -1 , the inoculum size was 5×10 5 cell / mL; The growth of Dunaliella salina can be divided into inoculation stage, vegetative growth stage and selenium conversion stage.

[0043] Described medium composition is as follows: H 3 BO3 : 9.28mg / L, CoCl 2 ·6H 2 O: 0.05mg / L, ZnCl 2 : 0.11mg / L, MnCl 2 4H 2 O: 1.98mg / L, Na 2 MoO 4 : 0.49mg / L, NaVO 3 : 0.24mg / L, CuCl 2 ·6H 2 O: 0.05mg / L, MgSO 4 ·7H 2 O: 1.23g / L, KCl: 0.2g / L, CaCl 2 2H 2 O: 0.044g / L, KNO 3 : 0.5g / L, KH 2 PO: 0.014g / L, FeCl 3 ·6H 2 O: 0.0005g / L, Na 2 EDTA: 0.074g / L, NaHCO 3 : 2.1g / L, NaCl: 87.7g / L. The culture medium added NaHCO 3 The previous pH was 7.5.

[0044] The volume of each medium is set to 150mL, and sodium selenite can be added to the medium so that the concentrat...

Embodiment 2

[0049] A preparation method of super selenium-enriched Dunaliella salina powder, comprising:

[0050] a) Cultivate Dunaliella salina in the culture medium at a temperature of 25±0.5°C and a light intensity of 50 μmol·m -2 the s -1 , the inoculum size was 5×10 5 cell / mL; The growth of Dunaliella salina can be divided into inoculation stage, vegetative growth stage and selenium conversion stage.

[0051] Described medium composition is as follows: H 3 BO 3 : 9.28mg / L, CoCl 2 ·6H 2 O: 0.05mg / L, ZnCl 2 : 0.11mg / L, MnCl 2 4H 2 O: 1.98mg / L, Na 2 MoO 4 : 0.49mg / L, NaVO 3 : 0.24mg / L, CuCl 2 ·6H 2 O: 0.05mg / L, MgSO 4 ·7H 2 O: 1.23g / L, KCl: 0.2g / L, CaCl 2 2H 2 O: 0.044g / L, KNO 3 : 0.5g / L, KH 2 PO: 0.014g / L, FeCl 3 ·6H 2 O: 0.0005g / L, Na 2 EDTA: 0.074g / L, NaHCO 3 : 2.1g / L, NaCl: 87.7g / L. The culture medium added NaHCO 3 The previous pH was 7.5.

[0052] The volume of each medium is set to 150mL, and sodium selenite can be added to the medium to control the conce...

Embodiment 3

[0057] A preparation method of super selenium-enriched Dunaliella salina powder, comprising:

[0058] a) Cultivate Dunaliella salina in the culture medium at a temperature of 25±0.5°C and a light intensity of 50 μmol·m -2 the s -1 , the inoculum size was 5×10 5 cell / mL; The growth of Dunaliella salina can be divided into inoculation stage, vegetative growth stage and selenium conversion stage.

[0059] Described medium composition is as follows: H 3 BO 3 : 9.28mg / L, CoCl 2 ·6H 2 O: 0.05mg / L, ZnCl 2 : 0.11mg / L, MnCl 2 4H 2 O: 1.98mg / L, Na 2 MoO 4 : 0.49mg / L, NaVO 3 : 0.24mg / L, CuCl 2 ·6H 2 O: 0.05mg / L, MgSO 4 ·7H 2 O: 1.23g / L, KCl: 0.2g / L, CaCl 2 2H 2 O: 0.044g / L, KNO 3 : 0.5g / L, KH 2 PO: 0.014g / L, FeCl 3 ·6H 2 O: 0.0005g / L, Na 2 EDTA: 0.074g / L, NaHCO 3 : 2.1g / L, NaCl: 87.7g / L. The culture medium added NaHCO 3 The previous pH was 7.5.

[0060] The volume of each medium is set to 150mL, and sodium selenite can be added to the medium to control the conce...

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Abstract

The invention discloses a super-selenium-rich dunaliella salina product and a preparation method and application thereof. The preparation method of the super-selenium-rich dunaliella salina product comprises the following steps of culturing, adding inorganic selenium and acquiring a finished product, wherein the culturing specifically comprises the steps of culturing the dunaliella salina in a culture medium; addition of inorganic selenium specifically comprises the steps of adding the inorganic selenium into the culture medium; and finished product acquisition specifically comprises the steps of continuously culturing for at least 3 days after the inorganic selenium is added, collecting and drying. According to the super-selenium-rich dunaliella salina product and the preparation method and the application thereof, inorganic selenium can be converted into organic selenium by adopting dunaliella salina, and the preparation process is simple and easy to implement; the content of organic selenium in the dunaliella salina can reach 1000mg / kg or above, the content of the organic selenium is 80%-99% of the total selenium content, and the standard of super-rich selenium is met; and meanwhile, the prepared super-rich selenium dunaliella salina product can be added into food, health care products and medicines at normal temperature, and can be absorbed and utilized by digestive systems of human bodies and animals.

Description

technical field [0001] The invention relates to the field of microalgae, in particular to a super-selenium-enriched Dunaliella salina product and a preparation method and application thereof. Background technique [0002] Selenium mainly exists in the form of organic selenium in organisms, and plays its role through selenium enzymes. For some lower organisms including microalgae and bacteria, as well as mammals, birds and fish, selenium is an essential micronutrient, and selenocysteine ​​(SeCys) is an active group that is part of the active site of selenoproteins. Selenium is a component of more than 30 kinds of human selenium enzymes. These enzymes participate in many important metabolic pathways including thyroid hormone metabolism, immune defense system, antioxidant defense and reproductive function, and participate in anti-cancer, biofilm protection, anti-aging, It has important biological functions such as scavenging free radicals and enhancing immunity. The World Hea...

Claims

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Application Information

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IPC IPC(8): C12P9/00C12N1/12C12R1/89
CPCC12P9/00C12N1/12
Inventor 胡章立郑怡鸿祝华昌李泽姜发军
Owner SHENZHEN UNIV
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