Super-selenium-rich dunaliella salina product and preparation method and application thereof
A technology of Dunaliella salina and selenium-enriched salts is applied in biochemical equipment and methods, microorganism-based methods, microorganisms, etc., which can solve the problems of undisclosed Dunaliella salina and other problems, and achieve the effect of simple and easy preparation process.
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Embodiment 1
[0041] A preparation method of super selenium-enriched Dunaliella salina powder, comprising:
[0042] a) Cultivate Dunaliella salina in the culture medium at a temperature of 25±0.5°C and a light intensity of 50 μmol·m -2 the s -1 , the inoculum size was 5×10 5 cell / mL; The growth of Dunaliella salina can be divided into inoculation stage, vegetative growth stage and selenium conversion stage.
[0043] Described medium composition is as follows: H 3 BO3 : 9.28mg / L, CoCl 2 ·6H 2 O: 0.05mg / L, ZnCl 2 : 0.11mg / L, MnCl 2 4H 2 O: 1.98mg / L, Na 2 MoO 4 : 0.49mg / L, NaVO 3 : 0.24mg / L, CuCl 2 ·6H 2 O: 0.05mg / L, MgSO 4 ·7H 2 O: 1.23g / L, KCl: 0.2g / L, CaCl 2 2H 2 O: 0.044g / L, KNO 3 : 0.5g / L, KH 2 PO: 0.014g / L, FeCl 3 ·6H 2 O: 0.0005g / L, Na 2 EDTA: 0.074g / L, NaHCO 3 : 2.1g / L, NaCl: 87.7g / L. The culture medium added NaHCO 3 The previous pH was 7.5.
[0044] The volume of each medium is set to 150mL, and sodium selenite can be added to the medium so that the concentrat...
Embodiment 2
[0049] A preparation method of super selenium-enriched Dunaliella salina powder, comprising:
[0050] a) Cultivate Dunaliella salina in the culture medium at a temperature of 25±0.5°C and a light intensity of 50 μmol·m -2 the s -1 , the inoculum size was 5×10 5 cell / mL; The growth of Dunaliella salina can be divided into inoculation stage, vegetative growth stage and selenium conversion stage.
[0051] Described medium composition is as follows: H 3 BO 3 : 9.28mg / L, CoCl 2 ·6H 2 O: 0.05mg / L, ZnCl 2 : 0.11mg / L, MnCl 2 4H 2 O: 1.98mg / L, Na 2 MoO 4 : 0.49mg / L, NaVO 3 : 0.24mg / L, CuCl 2 ·6H 2 O: 0.05mg / L, MgSO 4 ·7H 2 O: 1.23g / L, KCl: 0.2g / L, CaCl 2 2H 2 O: 0.044g / L, KNO 3 : 0.5g / L, KH 2 PO: 0.014g / L, FeCl 3 ·6H 2 O: 0.0005g / L, Na 2 EDTA: 0.074g / L, NaHCO 3 : 2.1g / L, NaCl: 87.7g / L. The culture medium added NaHCO 3 The previous pH was 7.5.
[0052] The volume of each medium is set to 150mL, and sodium selenite can be added to the medium to control the conce...
Embodiment 3
[0057] A preparation method of super selenium-enriched Dunaliella salina powder, comprising:
[0058] a) Cultivate Dunaliella salina in the culture medium at a temperature of 25±0.5°C and a light intensity of 50 μmol·m -2 the s -1 , the inoculum size was 5×10 5 cell / mL; The growth of Dunaliella salina can be divided into inoculation stage, vegetative growth stage and selenium conversion stage.
[0059] Described medium composition is as follows: H 3 BO 3 : 9.28mg / L, CoCl 2 ·6H 2 O: 0.05mg / L, ZnCl 2 : 0.11mg / L, MnCl 2 4H 2 O: 1.98mg / L, Na 2 MoO 4 : 0.49mg / L, NaVO 3 : 0.24mg / L, CuCl 2 ·6H 2 O: 0.05mg / L, MgSO 4 ·7H 2 O: 1.23g / L, KCl: 0.2g / L, CaCl 2 2H 2 O: 0.044g / L, KNO 3 : 0.5g / L, KH 2 PO: 0.014g / L, FeCl 3 ·6H 2 O: 0.0005g / L, Na 2 EDTA: 0.074g / L, NaHCO 3 : 2.1g / L, NaCl: 87.7g / L. The culture medium added NaHCO 3 The previous pH was 7.5.
[0060] The volume of each medium is set to 150mL, and sodium selenite can be added to the medium to control the conce...
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