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Sodium hyaluronate with full molecular weight distribution as well as preparation method and application of sodium hyaluronate

A technology of sodium hyaluronate and molecular weight distribution, which can be used in medical preparations containing active ingredients, applications, preparations for skin care, etc. Uniformity, product uniformity is difficult to guarantee and other problems, to achieve the effect of superior intelligent moisturizing, improving preparation efficiency and yield, and improving impurity removal efficiency

Active Publication Date: 2021-10-19
MEYER BIO-MEDICINE CO LTD +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, if the existing method wants to obtain sodium hyaluronate with a wider molecular weight distribution, it is necessary to mix and compound sodium hyaluronate of each molecular weight segment. However, the viscosity and other properties of sodium hyaluronate products with different molecular weights The difference is large, and the homogeneity of the product obtained by direct mixing is difficult to guarantee
Some physical, chemical or biological sodium hyaluronate degradation methods are also provided in the prior art. However, the molecular weight distribution of sodium hyaluronate treated by the existing degradation methods is not uniform, and the stability is poor, so it is difficult to further improve Quality of Sodium Hyaluronate Products

Method used

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  • Sodium hyaluronate with full molecular weight distribution as well as preparation method and application of sodium hyaluronate
  • Sodium hyaluronate with full molecular weight distribution as well as preparation method and application of sodium hyaluronate
  • Sodium hyaluronate with full molecular weight distribution as well as preparation method and application of sodium hyaluronate

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Experimental program
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Embodiment 1

[0035] This embodiment provides a method for preparing sodium hyaluronate with full molecular weight distribution, which specifically includes the following steps:

[0036] Step 1): Take 1 kg of food-grade sodium hyaluronate raw material with a molecular weight of 1 million, and evenly spray 50 mL of 1% hydrogen peroxide on the surface of the sodium hyaluronate raw material, using 300 μW / cm 2 Dose of ultraviolet radiation for 60min;

[0037] Step 2): Dissolving the sodium hyaluronate raw material after ultraviolet irradiation in water at a concentration of 1% (w / v), and stirring at room temperature to completely dissolve it;

[0038] Step 3): Use 2% NaOH solution to adjust the pH to 10, and then place it under 10 kHz ultrasonic treatment for 15 minutes;

[0039] Step 4): Prepare a 1 million sodium hyaluronate solution with a concentration of 1% (w / v), and mix it with the above-mentioned treated sodium hyaluronate alkaline solution at an addition ratio of 60% (v / v);

[0040] ...

Embodiment 2

[0044] This embodiment provides a method for preparing sodium hyaluronate with full molecular weight distribution, which specifically includes the following steps:

[0045] Step 1): Take 1 kg of food-grade sodium hyaluronate raw material with a molecular weight of 1.2 million, evenly spray 80 mL of 3% hydrogen peroxide on the surface of the sodium hyaluronate material, and use 600 μW / cm 2 Dose of ultraviolet radiation for 60min;

[0046]Step 2): Dissolving the sodium hyaluronate raw material after ultraviolet irradiation in water at a concentration of 3% (w / v), stirring at room temperature to completely dissolve it;

[0047] Step 3): Use 5% NaOH solution to adjust the pH to 12, and then place it under 30 kHz ultrasonic treatment for 30 minutes;

[0048] Step 4): Prepare a 1.2 million sodium hyaluronate raw material solution with a concentration of 0.8% (v / v), and mix it with the above-mentioned treated sodium hyaluronate alkaline solution at an addition ratio of 40%;

[0049...

Embodiment 3

[0053] This embodiment provides a method for preparing sodium hyaluronate with full molecular weight distribution, which specifically includes the following steps:

[0054] Step 1): Take 1 kg of cosmetic grade sodium hyaluronate raw material with a molecular weight of 1.2 million, evenly spray 80 mL of 5% hydrogen peroxide onto the surface of the sodium hyaluronate material, and use 900 μW / cm 2 Dose of ultraviolet radiation for 60min;

[0055] Step 2): Dissolving the sodium hyaluronate material after ultraviolet irradiation in water at a concentration of 5% (w / v), stirring at room temperature to completely dissolve it;

[0056] Step 3): Use 5% (w / v) NaOH solution to adjust the pH to 12, and then place it under 60kHz ultrasonic treatment, and the ultrasonic treatment time is 60min;

[0057] Step 4): Prepare a 1.2 million high molecular weight sodium hyaluronate solution with a concentration of 0.6% (w / v), and mix it with the above-mentioned treated sodium hyaluronate alkaline ...

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Abstract

The invention provides a preparation method of sodium hyaluronate with full molecular weight distribution, wherein the method comprises the steps: 1) carrying out hydrogen peroxide spraying and ultraviolet radiation treatment on a sodium hyaluronate solid raw material to obtain a sodium hyaluronate degradation material; 2) dissolving the sodium hyaluronate degradation material in water, and adjusting the pH value to be alkaline by using a NaOH solution to obtain a sodium hyaluronate alkaline solution; 3) performing ultrasonic treatment on the sodium hyaluronate alkaline solution; 4) preparing a sodium hyaluronate solution with the concentration of 0.1%-1% (w / v) from a hyaluronic acid solid raw material, and uniformly mixing the sodium hyaluronate solution with the sodium hyaluronate alkaline solution subjected to ultrasonic treatment according to the adding proportion of 20%-60% (v / v); and 5) performing adsorption treatment by adopting diatomite and activated carbon respectively, filtering and concentrating by adopting a nano filter membrane, and drying to obtain the product. According to the preparation method disclosed by the invention, sodium hyaluronate with relatively good activities such as water replenishing, moisturizing, anti-inflammation resistance and cell protection can be obtained.

Description

technical field [0001] The present application relates to the technical field of biomaterials, in particular to a sodium hyaluronate with a full molecular weight distribution and its preparation method and application. Background technique [0002] Hyaluronic acid (Haluronic acid, HA) is a linear polysaccharide linked by D-glucuronic acid and N-acetyl-D-glucosamine, with a molecular weight of hundreds of thousands to millions of daltons, widely distributed in In animal tissues, especially in human skin, umbilical cord, eye vitreous body and joint synovial fluid, the content is the highest, and it is also widely distributed in plants and microorganisms. In nature, HA has the same structure, no racial differences, and no immunogenicity. [0003] When HA is in a high concentration (1%), the intermolecular network exists in the form of a high viscoelasticity. The aqueous solution of HA is a viscoelastic fluid, which fills the space between cells and collagen fibers and covers ...

Claims

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Application Information

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IPC IPC(8): C08B37/08A23L29/00A23L33/125A61K8/73A61K31/728A61P17/00A61P29/00A61P43/00A61Q19/00
CPCC08B37/0072A61K31/728A61P17/00A61P29/00A61P43/00A61K8/735A61Q19/00A23L33/125A23L29/045A23L29/04A23V2002/00A23V2200/23A23V2200/30A23V2250/51A61P17/02B01D15/00A61Q19/08B01D61/027B01D2311/2626B01D2311/2699B01D61/04B01D69/02B01D2311/04B01D2325/34
Inventor 凌沛学邵华荣曾庆恺
Owner MEYER BIO-MEDICINE CO LTD
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