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Preparation method of electrochemical immunosensor based on polypyrrole modified silver phosphate loaded silver nanosphere composite material

An immunosensor and composite material technology, which is applied in the field of preparation of electrochemical immunosensors based on polypyrrole-modified silver phosphate-loaded silver nanosphere composite materials, can solve the problems of complex process, affect the biological activity of immune molecules, etc., and achieve reproducibility. Good, excellent anti-interference ability, high sensitivity effect

Inactive Publication Date: 2021-10-22
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The common construction process requires layer-by-layer modification, which is complicated and affects the biological activity of immune molecules

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Example 1 Preparation of Electrochemical Immunization Sensor

[0046] (1) Preparation of Prussae Blue Nucleus PBA

[0047] 0.1 g PVP and 90 mg K 3 [Fe (CN) 6 The mixed solution dispersed at 30 ml of ethanol and ultrapure water was 1: 2, and then 0.1 ml of concentrated hydrochloric acid was stirred for 10 min, then transferred to a 50 ml reaction kettle, and reacted at 80 ° C 12 h When cooled to room temperature, washed three times with ethanol, and finally dried to obtain blue product Prussian Blue Nucleus FEFEPBA;

[0048] (2) Preparation of nickel-iron Sprus Blue entries NiFE @ Fefe PBA

[0049] The above 10 mg FeFE PBA is dispersed in 20 mL ultrapure water, then add 0.1 g PVP, 100 mGnicl · 6h in turn 2 O and 0.1 g of citric acid, then 10 mg K K 3 [Fe (CN) 6 The solution was slowly added to the above solution, stirred for 10 min, allowed to age 24 h at room temperature, and finally washed three times with ultrapure water and ethanol, and obtained nickel Tmus Blue-like nuc...

Embodiment 2

[0069] Example 2 Preparation of Electrochemical Immunization Sensor

[0070] 1. A method of preparation of electrochemical immunosensor based on polypyrrole modified phosphoric acid silver load silver nanosphere composites, including the following steps:

[0071] (1) Preparation of Prussae Blue Nucleus PBA

[0072] 5.0 g PVP and 130 mg K 3 [Fe (CN) 6 The mixed solution dispersed at 30 mL of ethanol and ultrapure water was 1: 2, and then 2 ml of concentrated hydrochloric acid was stirred for 10 min, then transferred to a 50 mL reaction kettle, and reacted at 80 ° C 12 h When cooled to room temperature, washed three times with ethanol, and finally dried to obtain blue product Prussian Blue Nucleus FEFEPBA;

[0073] (2) Preparation of nickel-iron Sprus Blue entries NiFE @ Fefe PBA

[0074] The above 20 mg FeFE PBA is dispersed in 20 mL ultrapure water, then add 0.5 g of PVP, 150 mgNICL · 6H. 2 O and 0.5 g of citric acid, then 10 ml of 60 mg K 3 [Fe (CN) 6 The solution was slowly adde...

Embodiment 3

[0094] Example 3 Preparation of Electrochemical Immunization Sensor

[0095] 1. A method of preparation of electrochemical immunosensor based on polypyrrole modified phosphoric acid silver load silver nanosphere composites, including the following steps:

[0096] (1) Preparation of Prussae Blue Nucleus PBA

[0097] 10.0 g PVP and 150 mg K 3 [Fe (CN)6 The mixed solution dispersed at 30 mL of ethanol and ultrapure water is 1: 2, and then 5.0 ml of concentrated hydrochloric acid was stirred for 10 min, then transferred to a 50 mL reaction kettle, and reacted at 80 ° C 12 h When cooled to room temperature, washed three times with ethanol, and finally dried to obtain blue product Prussian Blue Nucleus FEFEPBA;

[0098] (2) Preparation of nickel-iron Sprus Blue entries NiFE @ Fefe PBA

[0099] The above 30 mg FeFE PBA is dispersed in 20 mL ultrapure water, and then 1.0 g PVP, 200 mGnicl · 6h sequentially 2 O and 1.0 g of citric acid, then the 20 ml of 100 mg k will 3 [Fe (CN) 6 The solu...

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Abstract

The invention relates to a preparation method of an electrochemical immunosensor based on a polypyrrole modified silver phosphate loaded silver nanosphere composite material. According to the invention, a primary antibody marker is formed by internal packaging of a Prussian blue analogue (NiFe@FeFe PBA) with a core-shell heterostructure and combination of a primary antibody externally loaded with luminol and a soluble fragment of cytokeratin 19; and a polypyrrole modified silver phosphate loaded silver nanosphere composite material (Ag3PO4@PPy-Ag) is combined with a second antibody of a soluble fragment of cytokeratin 19 to form a second antibody marker, so that the construction process of the sensor is simplified. Luminol is packaged into a porous core-shell heterostructure nanocube NiFe@FeFe PBA, so that the nano composite material with an excellent electrochemical luminescence behavior is obtained. Ag3PO4@PPy-Ag can effectively quench electrochemical luminescence of luminol, a sandwich quenching type electrochemical immunosensor is constructed based on the resonance energy transfer principle, ultra-sensitive detection of soluble fragments of cytokeratin 19 is achieved, and the detection limit is 28.43 fg mL <-1>.

Description

Technical field [0001] The present invention relates to a method for preparing an electrochemical immunosensor based on polypyrrole modified phosphate loaded silver nanosphere composite, specifically based on nuclear-shell-shell-sheated Pruscessar Blue analog NIFE @ Fefe PBA internal packaging and external load Anti-labeling of the Minino forms an anti-marker in a resistance to Cyfra 21-1 to polypyrroq, silver-free phosphoric acid silver loaded silver nanosphere composite material AG 3 PO 4 @ PPY-AG and the secondary anti-combination of CYFRA 21-1 form a secondary antibody, and a sandwich double-quenched electrochemical immunosensor that detects fine CYFRA 21-1 is prepared, which belongs to the field of new functional materials and biosensor detection. Background technique [0002] Lung cancer is a high degree of malignancy and rapid development of clinical tumors. It is mainly divided into small cell lung cancer and non-small cell lung cancer. The incidence of incidence is high ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/26G01N27/30G01N27/327
CPCG01N27/26G01N27/30G01N27/3278
Inventor 胡丽华崔倩倩宋翠师腾飞张勇马洪敏吴丹范大伟魏琴
Owner UNIV OF JINAN
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