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Acellular matrix fiber dressing and preparation method thereof

An acellular matrix and fiber technology, applied in the field of acellular matrix fiber dressing and its preparation, can solve the problems affecting wound recovery, infection, etc., and achieve the effects of increasing recovery effect, inhibiting colonization and uniform performance.

Inactive Publication Date: 2021-10-26
江苏优创生物医学科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, there is a risk of microbial colonization in acellular matrix dressings, which will affect the recovery of the wound and even cause infection

Method used

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  • Acellular matrix fiber dressing and preparation method thereof
  • Acellular matrix fiber dressing and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Such as figure 1 As shown, the present embodiment provides a spongy acellular matrix fiber dressing, the steps of its preparation comprising:

[0030] S1. Take the wet acellular matrix and cut it into a size less than 1cm 2 After the fragments, carry out high-speed mashing in a short time (no more than 1min);

[0031] S2. Take 0.5% (wet weight) of the acellular matrix, 0.01% glacial acetic acid and the rest of purified water by weight percentage, mix and cool down to 2°C;

[0032] S3. Perform homogenization at a frequency of 21 Hz, and then homogenize the collected feed liquid twice, filter out coarse particles, and use a 4% NaOH solution to adjust the pH of the filtered slurry to 6.5 , to obtain decellularized matrix fibers after centrifugation;

[0033] S4. Prepare an aqueous dispersion containing the acellular matrix fiber, adjust the pH to 4, add silver sulfate solution according to the solid content until the final mass fraction of silver in the acellular matrix...

Embodiment 2

[0035] This embodiment provides another spongy acellular matrix fiber dressing, the preparation steps of which include:

[0036] S1. Take the wet acellular matrix and cut it into a size less than 1cm 2 After the fragments, carry out high-speed mashing in a short time (no more than 1min);

[0037] S2. Take 3.0% (wet weight) of the acellular matrix, 0.1% glacial acetic acid and the rest of purified water by weight percentage, mix and cool down to 8°C;

[0038] S3. Perform homogenization at a frequency of 28 Hz, and then homogenize the collected material liquid twice, filter out coarse particles, and use a 6% NaOH solution to adjust the pH of the filtered slurry to 7.0 , to obtain decellularized matrix fibers after centrifugation;

[0039] S4. Prepare the aqueous dispersion containing the acellular matrix fiber, adjust the pH to 8, and add the silver chloride dispersion according to the solid content until the final mass fraction of silver in the acellular matrix fiber dressing...

Embodiment 3

[0041] Adopt GB 15979-2002 appendix C3 to detect the bactericidal performance of the acellular matrix fiber dressing prepared in Example 1, and the results are shown in the table below:

[0042] Table 1

[0043]

[0044] In summary, the present invention homogenizes the acellular matrix through fibrosis to obtain products with uniform properties; at the same time, silver salts are evenly dispersed, thereby slowly and continuously releasing silver ions when contacting wounds, which can inhibit gold The colonization of Staphylococcus aureus, Escherichia coli and Candida albicans increases the recovery effect of the wound.

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Abstract

The invention relates to an acellular matrix fiber dressing and a preparation method thereof. The preparation method comprises the following steps that a wet acellular matrix is taken, the acellular matrix is cut into fragments, and high-speed mashing is performed in a short time; the acellular matrix, glacial acetic acid and purified water are taken, mixing and cooling are achieved; homogenizing is achieved at a certain frequency, collected feed liquid is homogenized for a plurality of times, coarse particles are filtered out, the pH value of the filtered slurry is adjusted by using a NaOH solution, and centrifuging is achieved to obtain acellular matrix fibers; an aqueous dispersion liquid containing the acellular matrix fibers is prepared, a pH value is adjusted to 4-8, a solution or dispersion liquid of silver salt is added according to the solid content, uniformly mixing and dispersing are achieved, vacuum freeze drying is carried out, cutting, packaging and sterilizing are achieved to obtain the acellular matrix fiber dressing. According to the acellular matrix fiber dressing and the preparation method thereof, the acellular matrix is subjected to fibrosis and is homogenized, so that a product with uniform performance can be obtained; meanwhile, the silver salt is uniformly dispersed, so that silver ions are slowly and continuously released when the silver salt is in contact with a wound, the colonization of microorganisms can be inhibited, and the recovery effect of a wound surface is improved.

Description

technical field [0001] The invention relates to the technical field of biological materials, in particular to an acellular matrix fiber dressing and a preparation method thereof. Background technique [0002] Dressings derived from acellular matrices have been widely used for burn wound coverage and have shown potential in clinical applications. However, the acellular matrix is ​​derived from natural tissues, and there are not only individual differences, but also differences in different parts of the same individual. It is these differences that directly affect the performance of the acellular matrix. In addition, acellular matrix dressings also have the risk of microbial colonization, which can affect wound recovery and even cause infection. [0003] In the present application, a product with uniform performance can be obtained by fibrillating and homogenizing the decellularized matrix; at the same time, silver salt is evenly dispersed to inhibit microbial colonization. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L15/40A61L15/18A61L15/42A61L15/44A61L15/46
CPCA61L15/40A61L15/18A61L15/46A61L15/44A61L15/425A61L2300/104A61L2300/412A61L2300/602
Inventor 潘华倩潘银根吴锋陈件忠
Owner 江苏优创生物医学科技有限公司
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