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Domestication culture method of sulfur autotrophic short-cut denitrification sludge

A short-range denitrification and domestication culture technology is applied in the field of wastewater treatment to achieve the effects of rapid domestication method, low operating cost and improved total nitrogen removal efficiency

Inactive Publication Date: 2021-10-26
ZHENGZHOU UNIVERSITY OF LIGHT INDUSTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The purpose of the present invention is to provide a domestication and cultivation method of sulfur autotrophic short-range denitrification sludge for the coexistence of nitrogen pollutants and sulfides faced by existing sewage treatment. Enrich sulfur autotrophic denitrifying bacteria, control the substrate concentration and reaction time, realize the accumulation of nitrous, so as to realize the domestication and cultivation of sulfur autotrophic short-term denitrification sludge

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  • Domestication culture method of sulfur autotrophic short-cut denitrification sludge
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  • Domestication culture method of sulfur autotrophic short-cut denitrification sludge

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Embodiment 1

[0030] A domestication and cultivation method for sulfur autotrophic short-range denitrification sludge, comprising the following steps:

[0031] S1. Seed sludge selection: Inoculate the nitrification sludge taken from the aeration tank of the urban sewage plant in the intermittent flow reactor. The proportion of nitrifying bacteria contained in the nitrifying sludge is 5.1%, and the proportion of heterotrophic denitrifying bacteria is 12.9%. , the proportion of sulfur autotrophic denitrifying bacteria is 0.01%, the proportion of anammox bacteria is 0.01%, after concentration by gravity, the sludge concentration in the reactor is adjusted to 8g / L, and the mud-water volume ratio is 40% after sedimentation; Clean the muddy water in the reactor of sludge 3 times with pure water, and then place the reactor in a dark place for 48 hours;

[0032] S2. Early stage of domestication culture: Add wastewater containing ammonia nitrogen, nitrate nitrogen, sulfide and carbonate to the react...

Embodiment 2

[0039] A domestication and cultivation method for sulfur autotrophic short-range denitrification sludge, comprising the following steps:

[0040] S1. Seed sludge selection: Inoculate the nitrification sludge from the aeration tank of the urban sewage plant in the intermittent flow reactor. The proportion of nitrifying bacteria in the nitrifying sludge is 5.7%, and the proportion of heterotrophic denitrifying bacteria is 11.0%. , Sulfur autotrophic denitrifying bacteria and anammox bacteria were not detected, after concentration by gravity, adjust the sludge concentration in the reactor to 10g / L, after settling, the volume ratio of mud to water was 45%; wash the sludge with pure water for 3 times muddy water in S1, then place the reactor in a dark place for 60 hours;

[0041] S2. Early stage of domestication culture: Add wastewater containing ammonia nitrogen, nitrate nitrogen, sulfide and carbonate to the reactor, wherein the concentration of ammonia nitrogen is 15 mg / L, the c...

Embodiment 3

[0047] A domestication and cultivation method for sulfur autotrophic short-range denitrification sludge, comprising the following steps:

[0048] S1. Seed sludge selection: Inoculate the nitrification sludge from the aeration tank of the urban sewage plant in the intermittent flow reactor. The proportion of nitrifying bacteria in the nitrifying sludge is 6%, and the proportion of heterotrophic denitrifying bacteria is 12.3%. , the proportion of sulfur autotrophic denitrifying bacteria is 0.09%, and the proportion of anammox bacteria is 0.01%. After concentration by gravity, the sludge concentration in the reactor is adjusted to 7g / L, and the mud-water volume ratio is 30% after sedimentation; Clean the muddy water in the reactor of sludge 3 times with pure water, and then place the reactor in a dark place for 24 hours;

[0049] S2. Early stage of domestication culture: Add wastewater containing ammonia nitrogen, nitrate nitrogen, sulfide and carbonate to the reactor, wherein th...

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Abstract

The invention belongs to the technical field of wastewater treatment, and provides a domestication culture method of sulfur autotrophic short-cut denitrification sludge, wherein the method comprises the following steps: S1, seed sludge selection; S2, an earlier stage of domestication culture; S3, a middle stage of domestication culture; S4, a later stage of domestication culture; and S5, at the last stage of domestication culture, when the sulfur autotrophic denitrifying bacteria in the sludge become dominant bacteria, enabling sludge domestication to be successful, and enabling the proportion of the sulfur autotrophic denitrifying bacteria Thiobacillus sp. to be larger than 40%. The method disclosed by the invention is simple to operate, can be realized only by adding a trace amount of N-dodecanoyl-L-homoserine lactone and combining with water quality adjustment, and is low in operation cost; and the domestication method is rapid and efficient, nitrous accumulation can be achieved within 40 days, sulfur autotrophic short-cut denitrification sludge can be domesticated, and the method can be used for combined treatment of mixed wastewater containing ammonia nitrogen, nitrate nitrogen and sulfide.

Description

technical field [0001] The invention belongs to the field of wastewater treatment, and in particular relates to a domestication and cultivation method for sulfur autotrophic short-range denitrification sludge. Background technique [0002] The massive discharge of nitrogen-containing wastewater not only causes serious environmental pollution, such as water eutrophication, but also leads to potential human health hazards. Therefore, denitrification of wastewater is an urgent problem to be solved. The anaerobic ammonium oxidation process is a new sewage denitrification process. The process uses ammonia nitrogen as the electron donor and nitrous as the electron acceptor. Under the action of anammox bacteria, the reaction generates nitrogen gas. The reaction process is efficient and low consumption. However, this process produces 11% nitrate nitrogen, which limits the total nitrogen removal efficiency. If denitrification can be coupled, this part of nitrate nitrogen can be rem...

Claims

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Application Information

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IPC IPC(8): C02F3/30
CPCC02F3/305C02F3/307C02F3/301C02F2203/004
Inventor 张肖静张红丽马永鹏宋亚丽马闯张宏忠
Owner ZHENGZHOU UNIVERSITY OF LIGHT INDUSTRY
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