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Bacillus siamensis BsNlG13 for inhibiting pyricularia grisea and promoting seed germination and application thereof

A technology of bacillus and rice seeds, which is applied in the field of agricultural microorganisms to achieve good biological control potential and application prospects, improve germination rate, and improve the effects of germination rate.

Active Publication Date: 2021-10-26
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Chinese patent CN105505834A discloses a strain of Bacillus siamese for preventing and treating pear ring spot and soft rot, which has a certain bacteriostatic rate against blast fungus, but there are few reports on the influence of Bacillus siamese on the germination of rice seeds. to report

Method used

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  • Bacillus siamensis BsNlG13 for inhibiting pyricularia grisea and promoting seed germination and application thereof
  • Bacillus siamensis BsNlG13 for inhibiting pyricularia grisea and promoting seed germination and application thereof
  • Bacillus siamensis BsNlG13 for inhibiting pyricularia grisea and promoting seed germination and application thereof

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Experimental program
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Effect test

Embodiment 1

[0027] Isolation and cultivation of embodiment 1 bacterial strain BsN1G13

[0028] Dissected and plated: BsNlG13 strain was isolated from the intestinal tract of female adults of Nilaparvata lugens, and was isolated and cultured by dilution coating method. Specifically, dissect the intestinal tract of the female adult N. lugens planthopper under a stereomicroscope, place the dissected intestinal tract in PBS, grind it into a homogenate with a hand-held electric grinder, dilute it in four concentration gradients, and spread it on three culture media (LB medium, EB medium, NA medium), each treatment was repeated three times. They were cultured in a constant temperature incubator at 37°C and observed every 24 hours.

[0029] Continuous purification and culture photography: After a single colony grows in the selective medium, first pick a single colony according to the color, size and shape of the colony, and streak and purify it on the corresponding medium for more than 5 times,...

Embodiment 2

[0031] Identification and Phylogenetic Analysis of Example 2 Bacterial Strain BsN1G13

[0032] (1) Traditional biological identification:

[0033]Observed under an optical microscope, the colony shape is round or oval, the colony is milky white and translucent, the edge is complete, the colony is moist and slightly raised. The growth rate is faster at room temperature and 37°C, and the colony is larger after 12 hours of cultivation, with a diameter of 3-5mm.

[0034] (2) Determination of physiological and biochemical characteristics:

[0035] Bacterial physiological and biochemical assays were carried out with reference to the method in the "Common Bacterial System Identification Manual", using bacterial micro-biochemical reaction tubes. Microscopic observation shows that the bacterium is a rod-shaped, Gram-positive bacterium. The physiological and biochemical characteristics of the bacteria are shown in Table 1 below.

[0036] Table 1 Biochemical characteristics of BsNlG1...

Embodiment 3

[0046] The inhibitory activity of embodiment 3 bacillus siamese BsN1G13 to rice blast fungus

[0047] The inhibitory effect of Bacillus siamese BsN1G13 on Magnaporthe grisea was determined by plate confrontation culture method. Select solid medium as PDA medium. Inoculate the activated blast fungus MoZC13 bacterial sheet with a diameter of 5mm in the center of the PDA medium first, and inoculate the bacterial solution of the antagonistic Bacillus siamese BsN1G13 by streaking at a symmetrical place 2.5cm away from the bacterial sheet to inoculate only the blast fungus. The flat plate of MoZC13 bacterial slices was used as a control, and placed in a constant temperature incubator at 25°C for upside-down culture for 5 days under 75% humidity. The antibacterial effect of BsNlG13 was evaluated by measuring the diameter of the blast fungus colony after co-cultivation for 5 days. Perform biological replicates.

[0048] Tablet confrontation results such as image 3 A and image 3...

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Abstract

The invention discloses a bacillus siamensis BsNlG13 strain for inhibiting pyricularia grisea and promoting seed germination and application thereof. The bacillus siamensis BsNlG13 strain is preserved in Guangdong Microbial Culture Collection Center on December 25, 2020, and the preservation number of the bacillus siamensis BsNlG13 strain is GDMCC NO: 61391. The research shows that bacillus siamensis BsNlG13 strain fermentation liquor can effectively inhibit the growth of the pyricularia grisea, so that hyphae of the pyricularia grisea deform, and the normal growth of the pyricularia grisea cannot be realized; and meanwhile, the bacillus siamensis BsNlG13 strain fermentation broth has a significant promotion effect on rice seed germination. The bacillus siamensis BsNlG13 strain disclosed by the invention can be used as a good biocontrol and plant production regulation resource, is used for preventing and controlling the pyricularia grisea and promoting rice seed germination, and has a good application prospect.

Description

technical field [0001] The invention relates to the technical field of agricultural microorganisms, in particular to a Bacillus siamese BsN1G13 strain for inhibiting rice blast fungus and promoting seed germination and application thereof. Background technique [0002] As a very important food crop, rice is grown all over the world. Diseases that occur during rice cultivation are currently an important limiting factor for rice yield. Rice blast is an important rice fungal disease caused by the rice blast fungus Magnaportheoryzae, and it is one of the three major diseases of rice. Rice blast occurs in all rice regions in the world, and occurs more frequently in rice leaves and nodes. When the disease occurs, it can cause a large reduction in rice yield by 10%-30% every year, resulting in serious economic losses. At present, the prevention and control methods for rice blast are mainly to study the breeding of disease-resistant rice varieties or to use chemical agents for pr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/22A01P3/00A01P21/00C12R1/07
CPCC12N1/20A01N63/22
Inventor 许小霞金丰良王雪梅洪莹莹
Owner SOUTH CHINA AGRI UNIV
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