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Application of luteolin in preparation of drugs for targeted inhibition of ovarian cancer stem cells

A technology of ovarian cancer cells and luteolin, which is used in drug combinations, anti-tumor drugs, pharmaceutical formulations, etc., can solve the problems of high toxicity and side effects, tumor stem cell targeting inhibition, and lack of targeting of tumor stem cells. sexual effect

Pending Publication Date: 2021-11-02
CHONGQING UNIV CANCER HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, traditional chemotherapeutic drugs lack targeted inhibition of tumor stem cells and have large toxic and side effects, so they cannot be used for targeted inhibition of tumor stem cells. It is necessary to develop new drugs with clear tumor stem cell inhibitory effects and clear molecular mechanisms

Method used

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  • Application of luteolin in preparation of drugs for targeted inhibition of ovarian cancer stem cells
  • Application of luteolin in preparation of drugs for targeted inhibition of ovarian cancer stem cells
  • Application of luteolin in preparation of drugs for targeted inhibition of ovarian cancer stem cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Inhibitory effect of luteolin on ovarian cancer cells, expressed as half inhibitory concentration and maximum non-lethal concentration

[0033] (1) Culture of ovarian cancer cells

[0034] The human ovarian cancer cell line used in the experiment was Caov-3, and the cells were cultured in DMEM medium containing 10% fetal bovine serum and appropriate amount of antibiotics, and the culture conditions were 37°C and 5% CO 2 . Primary ovarian cancer cells #1 and #2 were isolated from the tumor tissue collected during the operation of ovarian cancer patients. The tumor tissue was cut into small pieces and digested with Dispase II in DMEM medium at 37°C for 30 minutes. After the cells were collected by centrifugation, Cultured in DMEM medium containing 10% fetal bovine serum and appropriate amount of antibiotics, the culture conditions were 37°C and 5% CO 2 .

[0035] (2) Study on the inhibitory effect of luteolin on ovarian cancer cells

[0036] Detect the inhibitory effe...

Embodiment 2

[0038] Inhibitory effect of luteolin on the ability of ovarian cancer stem cells to form microspheres

[0039] (1) Obtaining and culturing ovarian cancer stem cells

[0040] Caov-3 and #1 cells were inoculated into ultra-low adsorption culture plates respectively, and cultured with stem cell medium, which was DMEM / F-12 medium containing 10ng / ml FGF, 20ng / ml EGF, and appropriate amount of antibiotics. After the cells formed regular microspheres in the suspended state, the cells were collected by centrifugation, digested with trypsin-EDTA and gently pipetted repeatedly to form a single cell suspension of ovarian cancer stem cells.

[0041] (2) Study on the inhibitory effect of luteolin on the ability of ovarian cancer stem cells to form microspheres

[0042] Ovarian cancer stem cells cultured and isolated from Caov-3 and primary cell #1 were inoculated in an ultra-low adsorption six-well plate, and each well was inoculated with about 1×10 4cells. The experiment is divided int...

Embodiment 3

[0044] Inhibitory effect of luteolin on the frequency of microsphere formation of ovarian cancer stem cells in vitro

[0045] Caov-3 was cultured to isolate ovarian cancer stem cells. The experiment was divided into administration group and control group. Administration group: add 2 μM luteolin to the stem cell culture medium, and control group: add luteolin containing the same volume to the stem cell culture medium. Primer diluent solvent. After 24 hours of treatment, the medium was replaced, and 20, 10, and 5 cells / well were seeded in 96-well ultra-low adsorption culture plates. After continuing to cultivate for 25 days, count the number of wells forming microspheres, and use ELDA software to calculate the frequency of microspheres for each grade and composition. The results are as follows: Figure 4 As shown, the frequency of forming microspheres in the luteolin-administered group was significantly lower than that in the control group, which was statistically significant. ...

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Abstract

The invention relates to application of luteolin in preparation of drugs for targeted inhibition of ovarian cancer stem cells, and belongs to the field of tumor drug research. The invention provides a separation method of ovarian cancer stem cells, and finds that luteolin can inhibit the microsphere forming capacity, microsphere forming frequency, ovarian cancer stem cell marker expression level and tumor forming frequency of the ovarian cancer stem cells. In addition, the research finds that the molecular mechanism of luteolin for inhibiting the ovarian cancer stem cells is to inhibit the PPP2CA gene in the Hippo / YAP pathway of the ovarian cancer stem cells. The luteolin can be used for preventing and treating the ovarian cancer and improving the sensitivity of existing ovarian cancer treatment drugs, and has important significance in the field of ovarian cancer prevention and treatment.

Description

technical field [0001] The invention belongs to the field of tumor drug research and relates to the application of luteolin in the preparation of drugs targeted to inhibit ovarian cancer stem cells. Background technique [0002] Cancer is one of the major diseases that threaten human life, and ovarian cancer is the main disease that threatens women's lives. Ovarian cancer is caused by cell mutations in the ovary, and mutated tumor cells continue to proliferate in the ovary. As the disease progresses, ovarian cancer cells will migrate to other organs of the body and stay in the transferred organs to continue to proliferate. The hyperplasia of ovarian cancer cells leads to the obstruction of the normal function of the organs in the hyperplasia part of the patient, which eventually leads to the death of the patient. [0003] Currently, the treatment methods for ovarian cancer are mainly surgery, radiotherapy and chemotherapy. Surgery is to remove the tumor tissue formed by t...

Claims

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Application Information

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IPC IPC(8): A61K31/352A61P35/00
CPCA61K31/352A61P35/00
Inventor 郎廷元周琦李蕴哲邹冬玲王冬刘静姝丁东艳
Owner CHONGQING UNIV CANCER HOSPITAL
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