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Porous inorganic carrier and method for producing nucleic acid using same

An inorganic porous, inorganic porous technology, applied in the direction of inorganic chemistry, catalyst carrier, chemical instruments and methods, etc., can solve the problems of mixing by-products, reduction of synthesis efficiency, etc., and achieve the effect of improving purity

Pending Publication Date: 2021-11-09
SUMITOMO CHEM CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] In the solid-phase synthesis method, if the chain length of the nucleic acid to be synthesized becomes longer, the synthesis efficiency often decreases rapidly and a large amount of by-products (substances with a chain length shorter than the target chain length) are mixed in as a result.

Method used

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  • Porous inorganic carrier and method for producing nucleic acid using same
  • Porous inorganic carrier and method for producing nucleic acid using same
  • Porous inorganic carrier and method for producing nucleic acid using same

Examples

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preparation example Construction

[0059] For the preparation of the inorganic porous body, for example, silicates, alkoxysilanes, chlorosilanes, etc. are used as raw materials, and a solvent and a template are used to prepare by the above-mentioned synthesis method.

[0060] The preparation of the inorganic porous body can be carried out using, for example, any of the following methods: 1. After precipitating silica, the method of removing the solvent contained in the skeleton of silica; 2. Others, such as aluminum, boron and other different types of metals are mixed and solids are precipitated, and then phase-separated into silicon dioxide components and components other than silicon dioxide, and the method of removing components other than silicon dioxide; 3. Mixing Ammonium salts and polymers are used as templates to precipitate silica and then remove the template; or, 4. A method of aggregating the precipitated silica. Two or more of these methods may be used in combination.

[0061] As a method for remov...

Embodiment 1

[0289] 2.00 g of the inorganic porous body SP (1) was put into a four-necked bottle, and 100 mL of toluene was added. With stirring, 4.8 mg of the component (C1) was further added, followed by stirring at room temperature for 3 hours. Thereafter, the reaction solution was filtered, washed with toluene, and then dried under reduced pressure to obtain the inorganic porous carrier of Example 1.

Embodiment 2

[0291] Except having changed the inorganic porous body SP (1) into the inorganic porous body SP (2) (2.00g), it carried out similarly to the manufacturing method of Example 1, and obtained the inorganic porous support body of Example 2.

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Abstract

The present invention provides a porous inorganic carrier that further increases the purity of nucleic acid during production thereof. The porous inorganic carrier comprises a linker represented by general formula (1) (wherein the bond marked with the * represents a bond to the oxygen atom of a silanol group in the porous inorganic body; n represents an integer, e.g., 1; each R independently represents a C3-10 alkyl group optionally having a substituent, e.g., an alkoxy group; and L represents a single bond, a C1-20 alkylene group, or a C2-20 alkylene group that contains the group: -CH2-Q-CH2- as provided by the insertion of any group Q selected from the group consisting of -O- and so forth, in at least one -CH2-CH2- group present in said alkylene group), has a mode diameter of 0.04 [mu]m to 1 [mu]m, in the pore distribution measured by a mercury intrusion method, and has a predetermined cumulative pore volume percentage of not more than 30%. The present invention also provides a method for producing a nucleic acid using the porous inorganic carrier.

Description

technical field [0001] This patent application claims the priority and rights under the Paris Convention based on Japanese Patent Application No. 2019-067995 (filed on March 29, 2019), and all the contents described in the above application are hereby incorporated by reference. middle. [0002] The present invention relates to an inorganic porous carrier and a method for producing a nucleic acid using the same. Background technique [0003] As a method for chemically synthesizing nucleic acids, a solid-phase synthesis method based on the phosphoramidite method has been widely used. In this method, first, a functional group such as an amino group is introduced into an inorganic porous body using a silane coupling agent or the like, and a nucleoside serving as the 3' terminal of the nucleic acid is bonded to the functional group. Thereafter, a nucleic acid extension reaction is performed on a solid-phase support using the aforementioned nucleoside as a starting point. [00...

Claims

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Application Information

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IPC IPC(8): B01J31/26B01J32/00C01B33/12C01B39/36C07F7/18C07H21/04C12N15/10
CPCC01B39/00C07H21/00C01P2006/14C01P2006/16C01P2006/12C01P2004/60C01B33/157C01B33/18C03C23/00C07H1/00B01J21/08B01J27/24B01J2229/32B01J35/60C07F7/18C07H1/02C07H21/04
Inventor 北原真树吉冈健原孝志有村孝石山武岸田宽
Owner SUMITOMO CHEM CO LTD
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