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Pesticide composition with synergistic effect and for preventing and treating litchi downy blight

A pesticide composition and synergistic technology, applied in the direction of resistance to vector-borne diseases, chemicals for biological control, application, etc., can solve problems such as environmental pollution, improve control effects, reduce usage, and increase toxicity. force effect

Active Publication Date: 2021-11-19
ENVIRONMENT & PLANT PROTECTION INST CHINESE ACADEMY OF TROPICAL AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above-mentioned inventions will cause certain pollution to the environment, and also need to add other preparations

Method used

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  • Pesticide composition with synergistic effect and for preventing and treating litchi downy blight
  • Pesticide composition with synergistic effect and for preventing and treating litchi downy blight
  • Pesticide composition with synergistic effect and for preventing and treating litchi downy blight

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] The synergistic effect of farnesol or trans-farnesol on dimethomorph was determined by mycelium growth rate method. Use the solvent acetone to dissolve the test compound under sterile conditions, and prepare the mother solution of bactericide and synergist at 1000 μg / mL -1 spare. The pharmaceutical composition is as follows: dimethomorph: farnesoid (or trans-farnesoid) = 1:1, 2:1, 4:1, 8:1, 16:1 (an equal volume of solvent is used as a control). Mix the drug into the lima bean medium to prepare a plate containing the drug. After the plate is solidified, insert a 4mm bacterial plate (taken from the edge of the colony that has grown normally for 7 days at 25°C in the dark) and place it at 25°C for dark culture. 4d. Each treatment was repeated 3 times. The synergistic effect is judged by the Wadley synergistic coefficient, and the calculation method of the Wadley synergistic coefficient is shown in formulas (1) to (3).

[0019] (1)

[0020] (2)

[0021] (3)

...

Embodiment 2

[0026] The synergistic effect of farnesol and dimethomorph on P. litchi peronospora was determined by the method of detached leaves. Select young litchi leaves with the same leaf position and similar area and size, wash them with water, dry them, immerse them in 75% alcohol for disinfection for 10 seconds, rinse them with sterile water 3 times, and dry them for later use.

[0027] Use solvent acetone to dissolve the test compound, prepare dimethomorph and farnesol mother solution 1000μgmL -1 spare. The pharmaceutical formulation ratio is as follows: dimethomorph: farnesol = 0:1, 2:1, 8:1, 1:0 (an equal volume of solvent is taken as a control). The concentration of each proportioning drug is set to 50μgmL -1 , completely immerse the leaves in each fungicide agent for 10 seconds, make it fully contact with the liquid agent, and then take it out to dry. Use a 0.2mm diameter inoculation needle to create a wound at the vein on the back of the blade, and inoculate two bacterium c...

Embodiment 3

[0033] The field control effect test of the combination of farnesol and dimethomorph on litchi frost blight was carried out with reference to the "Guidelines for Field Efficacy Tests of Pesticides". The field test site is Huidong Niannianhong Orchard, Baihua Town, Huidong County, Huizhou City, from May to June 2020, and the tested variety is Guiwei. The test agent is dimethomorph: farnesol=2:1, 8:1, and the treatment dosage is respectively active ingredient 250mgL -1 ; With 68% fine nail cream · manganese zinc WG (treatment dosage active ingredient is 850mgL -1 ) and 30% enoyl prochloraz SC (the active ingredient in the treatment dose is 500mgL -1 ) as the control agent, and treated with water as the blank control group. Each treatment was repeated 4 times, arranged in random blocks, and the cultivation conditions and growth of all test litchi trees were consistent. On May 11, 2020 (small fruit period), May 23 (medium fruit period) and June 05 (fruit expansion period), the ...

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Abstract

The invention provides a pesticide composition with the synergistic effect and for preventing and treating litchi downy blight. The composition for preventing and treating the litchi downy blight is prepared from farnesol and dimethomorph, the farnesol can inhibit formation and maturation of fungal biofilms and induce death of fungal cells, when the farnesol is singly used, the farnesol basically has no obvious control effect on the litchi downy blight, and the carboxylic acid amide bactericide dimethomorph is used as a registered medicament for controlling the litchi downy blight, so that the control effect is obviously improved after the farnesol is added.

Description

technical field [0001] The invention relates to the field of preventing and treating litchi frost blight, in particular to a pesticide composition with synergistic effect for preventing and controlling litchi frost blight. Background technique [0002] Lychee downy mildew belongs to Pseudobacteria, Oomycetes, Oomycetes, Peronomycetes, Peronomycetes. The litchi downy blight caused by this pathogen is the most important disease in the litchi industry chain, and it is endemic and explosive. It often causes a large amount of fruit drop and rotten fruit, especially when it is rainy in May and June, the occurrence of this disease can cause 30-80% loss to litchi planting in Guangdong Province. [0003] To prevent and control oomycete disease, due to the lack of disease-resistant varieties, chemical fungicides are still one of the most effective methods to prevent and control the disease. At present, there are 65 kinds of pesticide preparations registered in my country for the prev...

Claims

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Application Information

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IPC IPC(8): A01N37/38A01N49/00A01P3/00
CPCA01N37/38A01N49/00Y02A50/30
Inventor 张静文小东谷梦萱朱发娣
Owner ENVIRONMENT & PLANT PROTECTION INST CHINESE ACADEMY OF TROPICAL AGRI SCI
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