Preparation and application of antibacterial and anti-inflammatory plant extract composition

A technology of plant extraction and composition, applied in the direction of drug combination, anti-inflammatory agent, skin care preparation, etc., to achieve the effect of eliminating inflammatory factors

Pending Publication Date: 2021-11-30
COSMAX CHINA INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] Existing research rarely mentions the antibacterial application of basil, chlorogenic acid and medicinal rhubarb in the field of cosmetics

Method used

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  • Preparation and application of antibacterial and anti-inflammatory plant extract composition
  • Preparation and application of antibacterial and anti-inflammatory plant extract composition
  • Preparation and application of antibacterial and anti-inflammatory plant extract composition

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Embodiment 1. The preparation of the plant extract composition that can effectively anti-inflammatory and antibacterial

[0052] In the following examples and comparative examples of the present invention, the plant extract composition capable of effectively anti-inflammatory and antibacterial is prepared by the following process.

[0053] (1) the rhubarb raw material is pulverized according to the mass ratio;

[0054] (2) Add deionized water for extraction, the mass ratio of raw material to deionized water is 1:20-1:50, soak for 1-3 hours. Water bath, add cellulase when the temperature is 40-50°C, the total mass of the added enzyme accounts for 10%-20% of the total mass of the raw material, stir for 20 minutes, heat up to 40-60°C and microwave for 5-15 minutes, ultrasonic water bath Reflux extraction for 1-3 hours;

[0055] (3) cooling the extract obtained in step (2) to 20-30° C., and filtering with 100-200 mesh gauze to obtain primary filtrate 1;

[0056] (4) Cut ...

Embodiment 2

[0061] Example 2. Composite Antioxidant Efficacy Evaluation of Different Ratio Compositions

[0062] (1) the rhubarb raw material is pulverized according to the mass ratio;

[0063] (2) Add deionized water for extraction, the mass ratio of raw material to deionized water is 1:20, soak for 2 hours. Water bath, add cellulase when the temperature is 48°C, the total mass of the added enzyme accounts for 10% of the total mass of raw materials, stir for 20 minutes, heat up to 54°C for microwave treatment for 10 minutes, and reflux extraction in an ultrasonic water bath for 3 hours;

[0064] (3) cooling the extract obtained in step (2) to room temperature, and filtering with 200 mesh gauze to obtain the initial filtrate 1;

[0065] (4) Cut the fresh basil into 1-2cm sections according to the mass ratio, soak it with 3 times the amount of 95% ethanol solution for 1 hour, extract it through ultrasonic vibration at low temperature, filter, and combine the filtrates to obtain the primar...

Embodiment 3

[0084] Example 3. Detection of cellular inflammation

[0085] (1) MTT cytotoxicity test

[0086] According to 1X10 in 96well multi plate (corning) 4 DMEM medium containing 10% bovine serum and 100 μL of keratinocytes (HaCaT) were inoculated at the density of cells / well, and replaced with serum-free medium after 24 hours of culture. Compositions 6, 8, and 10 in the above examples were added to the serum-free medium for treatment and cultured for 24 hours. Thereafter, the medium was removed, treated with 20 μL of MTT solution, and allowed to react at 37° C. for 2 hours. Add 200 μL of isopropanol to the cells from which the MTT solution was removed, and shake gently for 30 minutes to completely dissolve the crystalline formazan, measure the absorbance at 570 nm, and calculate the cell survival rate according to the following formula.

[0087]

[0088] The control group was tested without adding samples. The cytotoxicity-related results are shown in Table 3 below.

[0089...

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Abstract

The invention discloses an antibacterial and anti-inflammatory plant extract composition and application thereof. The antibacterial and anti-inflammatory plant extract composition is prepared by taking basil, chlorogenic acid and medicinal rhubarb as raw materials according to a compound ratio, and performing related tests by taking the antibacterial activity and the addition amount of the composition as entry points, so as to finally obtain the plant extract composition which is small in addition amount and strong in antibacterial and anti-inflammatory effects, wherein the mass ratio of the basil to the chlorogenic acid to the medicinal rhubarb is (1-3): (0.05-0.2): (1-3). The composition disclosed by the invention can effectively inhibit the breeding of skin pathogenic bacteria, and can weaken inflammatory reactions by reducing reactive oxygen species, eliminating inflammatory factors, improving cuticle moisture loss and the like.

Description

technical field [0001] The invention belongs to the field of cosmetics and relates to the preparation and application of an antibacterial and anti-inflammatory plant extract composition. Background technique [0002] Due to the improvement of people's lifestyle, the constant change of living environment and the influence of age, psychological pressure, environmental pollution, seasonal changes, chemical abuse, etc., it has also brought many injuries to our skin and skin mucous membranes, and the incidence of skin diseases The incidence rate shows a rising trend. Modern medical research has shown that the imbalance of human flora and the growth of pathogenic bacteria will lead to endocrine dysfunction. The reaction on the skin often results in strong sebum secretion, inflammation, acne redness, and small pustules on the top. [0003] Basil (Ocimum basilicum) is a plant of the Lamiformes, Lamiaceae, and genus Ocimum. It is also known as smoked grass, larkspur, cymbidium, and ...

Claims

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Application Information

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IPC IPC(8): A61K8/9789A61K8/37A61Q19/08A61Q17/00A61P29/00
CPCA61K8/375A61K8/9789A61Q17/005A61Q19/08A61P29/00A61K2800/805A61K2800/82Y02A50/30
Inventor 曹茜周秋娜金荣熙姚清申彦晟金延埈
Owner COSMAX CHINA INC
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