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Exosome preparation suitable for mucosa and having similar needle-free injection effect and preparation method of exosome preparation

A needle-free injection, exosome technology, applied in the field of biomedicine, can solve the problems of reducing the expected curative effect, drug loss, reducing the contact time between drugs and vaginal mucosa, etc., achieving good pertinence and adaptability, regulating menstruation, and increasing penetration. The effect of the skin effect

Active Publication Date: 2021-12-03
陕西科美致尚生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, under normal circumstances, there are a variety of bacteria in the vaginal environment that grow in a certain proportion. For example, lactic acid bacteria, one of the main species, are responsible for maintaining the acidic environment of the vagina, keeping the pH at 4-4.5, and preventing pathogenic bacteria from multiplying in the vagina; And the vagina can secrete mucus to clean itself. The secretion of mucus will lead to the loss of drugs, reduce the contact time between drugs and vaginal mucosa, and reduce the expected curative effect

Method used

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  • Exosome preparation suitable for mucosa and having similar needle-free injection effect and preparation method of exosome preparation
  • Exosome preparation suitable for mucosa and having similar needle-free injection effect and preparation method of exosome preparation
  • Exosome preparation suitable for mucosa and having similar needle-free injection effect and preparation method of exosome preparation

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Experimental program
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Effect test

Embodiment 1

[0048] An exosome preparation suitable for mucosal injection with a needle-free injection effect, prepared according to the following steps:

[0049] Step 1, co-culture of stem cells and lactic acid bacteria

[0050] 1) Preparation of lactic acid bacteria-sodium alginate gel balls

[0051] Prepare a sodium alginate solution with a mass concentration of 5% and a CaCl solution with a mass concentration of 4% 2 solution. The two solutions were sterilized at 121°C for 20 minutes under high temperature and high pressure, and then placed in a sterile operating table to cool to room temperature.

[0052] Weigh an appropriate amount of lactic acid bacteria, put them into a sterilized beaker, mix evenly with sodium alginate solution, the final wet weight concentration of lactic acid bacteria is 1% (w / w), draw the mixed solution with a syringe or pipette, and drop evenly to CaCl 2 In solution, the surface of the droplet calcifies into balls with a diameter of 1-3 mm, see figure 1 . ...

Embodiment 2

[0073] A method for preparing an exosome preparation with a needle-free injection effect suitable for mucosa, comprising the following steps:

[0074] Step 1, co-culture of stem cells and lactic acid bacteria

[0075] 1) Preparation of lactic acid bacteria-sodium alginate gel balls

[0076] Prepare a sodium alginate solution with a mass concentration of 3% and a CaCl solution with a mass concentration of 2% 2 solution. The two solutions were sterilized at 121°C for 20 minutes under high temperature and high pressure, and then placed in a sterile operating table to cool to room temperature.

[0077] Refer to Example 1 for the remaining operations of step 1, wherein the crosslinking time is changed to 12h.

[0078] 2) Stem cell inoculation

[0079] The P3 passage umbilical cord mesenchymal stem cells were cultured in 4×10 4 Cells / mL were inoculated in the culture container; or, the P6 generation umbilical cord mesenchymal stem cells were inoculated at 7×10 4 Inoculate the ...

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Abstract

The invention belongs to the technical field of biological medicine, and particularly relates to an exosome preparation suitable for mucosa and having a similar needle-free injection effect and a preparation method of the exosome preparation. Mesenchymal stem cells and lactic acid bacteria are co-cultured, supernatant is collected, cell factors and exosomes secreted by the mesenchymal stem cells are sequentially separated according to molecular weight through an ultrafiltration concentration technology, wherein the cell factors are encapsulated by lipidosome and coated with S-layer protein, then the cell factors and the exosomes are mixed in proportion, freeze-dried powder is prepared, and then the dry powder spray is prepared. The dry powder spray has a transdermal absorption effect similar to needle-free injection, is not easy to lose through vaginal mucosa administration, and can greatly reduce loss.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to an exosome preparation and a preparation method suitable for mucosa and having a needle-free injection effect. Background technique [0002] With the development of biomacromolecular drugs, how to safely and effectively deliver drugs has gradually become a research hotspot. Oral intake of biomacromolecules will decompose and fail through the digestive tract; injection with a needle will cause significant tissue damage, and the pain is obvious, and the patient's compliance is low, which is not conducive to the daily use of the drug. Therefore, biomacromolecule needle-free injection technology has received widespread attention. This technology does not use needles when delivering drugs, and uses the instantaneous high pressure generated by mechanical devices to instantly pass the drug through the patient's epidermal cells in the form of liquid needles, making the dr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/19A61K35/28A61K47/10A61K47/26A61P15/02A61P31/02C12N1/20C12N5/0775C12N11/10A61K9/127A61K9/14A61K38/19A61K45/06A61K31/722A61K47/36
CPCC12N5/0662C12N1/20C12N11/10A61K9/19A61K35/28A61K47/10A61K47/26A61K9/127A61K45/06A61K9/0034A61K9/146A61P15/02A61P31/02A61K31/722A61K38/19A61K2300/00Y02A50/30
Inventor 王哲明磊国王清霞董玲娟姜勃白天君
Owner 陕西科美致尚生物科技有限公司
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