Method for detecting salicylic acid by using fluorescent micro-probe and application
A technology of salicylic acid and fluorescent microspheres, applied in the direction of fluorescence/phosphorescence, chemical instruments and methods, luminescent materials, etc., can solve the problems of slow response time of fluorescent probes, complicated operations, skin damage, etc., and improve the stability of fluorescent signals Excellent performance and sensing performance, simple detection process, and accurate detection results
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Embodiment 1
[0036] The preparation of embodiment 1 fluorescent microsphere and fluorescent microprobe
[0037] (1) Preparation of fluorescent microspheres
[0038] In a 250mL round bottom flask, dissolve 0.10g (0.30mmol) hexachlorocyclotriphosphazene (HCCP) and 0.32g (0.90mmol) curcumin in 50mL acetonitrile until hexachlorocyclotriphosphazene and curcumin are completely dissolved in After reaching the acetonitrile solution, 2 mL of triethylamine (about 27 mmol) was added, and stirred at room temperature for 8 h. After the reaction is complete, centrifuge to obtain the lower sediment, wash with ethanol and pure water until the supernatant is clear, dry the obtained sediment in vacuum at 50°C for 12 hours, and obtain a light yellow powder with a particle size of 0.5-2 microns. The technical route is :
[0039]
[0040] (2) Preparation of fluorescent microprobe PC-Al(III)
[0041]
[0042] A PC solution with a concentration of 12 mg / mL was prepared with ethanol solution, and an Al(N...
Embodiment 2
[0043] Embodiment 2 Fluorescence-specific selectivity
[0044] Prepare 12mg / mL PC ethanol solution and 100mg / mL Al(III) aqueous solution, add 2mL ethanol solution, 10μL PC solution and 10μL Al(III) aqueous solution to the cuvette, and investigate the probe with a fluorescence spectrometer Selectivity of PC-Al(III) to small molecules such as salicylic acid. like figure 1 As shown, under the excitation condition at 313nm, the independent probe PC-Al(III) has a weak fluorescence emission intensity at 400nm in ethanol solution, and after adding salicylic acid, the fluorescence emission intensity at 400nm is obviously enhanced , when other substances were added, the fluorescence emission intensity of the solution system did not change significantly compared with the fluorescence emission intensity of the individual probe system, indicating that the probe PC-Al(III) had better fluorescence specificity for salicylic acid in ethanol solution. One optional.
Embodiment 3
[0045] Embodiment 3 anti-interference test
[0046] Prepare 12mg / mL PC ethanol solution and 100mg / mL Al(III) aqueous solution, add 2mL ethanol solution, 10μL PC solution and 10μL Al(III) solution to 18 clean fluorescent cuvettes respectively , and then add 10 μM salicylic acid and 100 μM other analytes, such as:
[0047]Other small molecules: salicylamide (SAM), benzoic acid (BA), p-hydroxybenzoic acid (4-HBA);
[0048] Cation: Na + 、K + , Ca 2+ , Mg 2+ 、Co 2+ 、Ni 2+ 、Cu 2+ , Zn 2+ , Sn 2+ , Fe 3+ ;
[0049] Anion: Cl - , NO 3 - 、ACO - 、HCO 3 - , CO 3 2- , detected on a fluorescence spectrometer, and draw the fluorescence emission histogram of the highest fluorescence intensity corresponding to different analytes, such as figure 2 As shown, it is proved that the recognition of salicylic acid in ethanol solution by the probe PC-Al(III) is not interfered by the above-mentioned other analytes, and has good anti-interference.
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