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Method for efficiently detecting and identifying plant volatile matters by utilizing GC-MS/MS

A high-efficiency technology for plant volatiles, applied in the field of analytical chemistry, can solve problems such as poor reproducibility of results, limited detection throughput and quantity of qualitative substances, and inability to effectively improve the detection and qualitative flux of volatile groups, and achieve data Ease of integration, improved quantitative accuracy, and avoidance of convolution problems

Pending Publication Date: 2021-12-03
罗杰
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] However, the detection throughput and the number of qualitative substances in the wide-target method are still limited by the non-target method, and the direct transfer of the wide-target method idea cannot effectively improve the detection and qualitative throughput of the volatile group; in addition, based on SPME-GC-MS The volatile group method has the problem of poor reproducibility of results

Method used

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  • Method for efficiently detecting and identifying plant volatile matters by utilizing GC-MS/MS
  • Method for efficiently detecting and identifying plant volatile matters by utilizing GC-MS/MS
  • Method for efficiently detecting and identifying plant volatile matters by utilizing GC-MS/MS

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Embodiment 1

[0044] A method utilizing GC-MS / MS to efficiently detect and identify plant volatiles, the steps of which are:

[0045] 1. Sample preparation:

[0046] (1) Sampling. Granular samples: 2.0g Nipponbare rice seeds, 2.0g Hanmai 19 wheat seeds were shelled and stored at room temperature, 2.0g Zhengdan 958 corn were threshed and stored at room temperature; powder samples: 2.0g durian pulp, Reyin 1 No. pepper fruit was ground into powder by adding liquid nitrogen in a mortar, and stored in liquid nitrogen. Durian was purchased from a local supermarket; liquid samples: 4 mL of tomato, citrus, apple, pear, and passion fruit juices were squeezed by a juicer, and the juice was extracted at 4 Store at ℃ and test within 12 hours. Tomatoes, citrus, apples, pears, and passion fruit are purchased from local supermarkets;

[0047] (2) Solution preparation: the internal standard solution is V 水 :V TMP =6×10 6 : 1; powdered sample extract is V 水 :V EDTA :V TMP =1000:1:0.001, V stands for...

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Abstract

The invention discloses a method for efficiently detecting and identifying plant volatile matters by utilizing GC-MS / MS. The method comprises the following steps: firstly, detecting the plant volatile matters of multiple species by utilizing a full-scanning mode, deconvolving obtained data, and determining the nature of the matters; secondly, selecting precursor ions of all the substances, detecting product ions of all the precursor ions in a product ion mode, and optimizing collision voltage; thirdly, integrating the obtained ion pairs into a single MRM (multiple reaction monitoring mode) method; and finally, establishing a correction model suitable for volatile matter detection. By using the method, the sensitivity, the detection flux, the qualitative substance quantity, the quantitative accuracy and the method reproducibility during volatile matter detection can be effectively improved. The method is high in applicability, the volatile matter detection effect is remarkably improved, and the method has great significance in research of plant volatile matters.

Description

technical field [0001] The invention belongs to the field of analytical chemistry, and more specifically relates to a method for efficiently detecting and identifying plant volatiles by using GC-MS / MS, which can significantly improve the detection effect of plant volatiles. Background technique [0002] Plant volatiles are a class of lipophilic liquids with high saturated vapor pressure. More than 1700 plant volatile compounds have been identified (Natalia Dudareva et al., Biosynthesis, function and metabolic engineering of plant volatile organic compounds. New Phytologist, 2013). Plant volatiles play an important role in both plants and humans: in plants, volatiles play a role in the interaction with the environment (AinoKalske et al., Insect herbivory selects for volatile-mediated plant-plantcommunication. Current Biology, 2019); For humans, plant volatiles provide food aroma, and can also be used as spices, condiments, medicines, etc. (Eran Pichersky et al., Biosynthesis...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N30/34G01N30/86
CPCG01N30/02G01N30/06G01N30/34G01N30/8679
Inventor 罗杰袁弘伦曹光平刘贤青候晓东黄梦兰杜鹏萌
Owner 罗杰
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