Method for rapidly detecting myclobutanil residues in food and magnetic surface molecular imprinting adsorbent used by method

A surface molecular imprinting and adsorbent technology, which is applied in the field of magnetic surface molecular imprinting adsorbents, can solve problems affecting the sensitivity of detection results, etc., and achieve the effects of saving pretreatment time and cost, good impurity removal effect, and high sensitivity

Pending Publication Date: 2021-12-14
合肥海关技术中心 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Fruits, vegetables, grains, tea, fruit and vegetable juices and other food matrices are complex, and some pigments, organic

Method used

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  • Method for rapidly detecting myclobutanil residues in food and magnetic surface molecular imprinting adsorbent used by method
  • Method for rapidly detecting myclobutanil residues in food and magnetic surface molecular imprinting adsorbent used by method
  • Method for rapidly detecting myclobutanil residues in food and magnetic surface molecular imprinting adsorbent used by method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1: A method for rapid detection of myclobutanil residues in food and its magnetic surface molecular imprinting adsorbent used

[0034] A kind of rapid detection method of myclobutanil residue in apple juice, the step comprises:

[0035] Step 1. Weigh 2.0 g of the sample and add it to a 50 mL centrifuge tube, then add 10 mL of acetonitrile-water (1:4; V:V), vortex and oscillate for extraction for 2 min, and centrifuge at 4000 r / min for 5 min.

[0036] Step 2. Take 5 mL of the supernatant, add 50 mg of magnetic surface molecular imprinting adsorbent, and shake and extract for 20 min. The magnetic surface molecular imprinting adsorbent was gathered to the bottom with a magnet, all the solution was poured carefully, and the adsorbent was washed twice with acetonitrile-water (1:4, V:V).

[0037] Step 3. Transfer the adsorbent to a 2mL centrifuge tube, add 1mL acetonitrile solution and vortex for 1min for desorption, and collect the analysis solution. The desorption...

Embodiment 2

[0048] Example 2: A method for rapidly detecting myclobutanil residues in food and the magnetic surface molecular imprinting adsorbent used therefor A rapid detection method for myclobutanazole residues in grapes, the steps include:

[0049] Step 1. Homogenize the grape sample to 100 mesh.

[0050] Step 2. Weigh 2.0 g of the sample and add it to a 50 mL centrifuge tube, then add 20 mL of acetonitrile-water (1:4, V:V), vortex and oscillate for extraction for 3 min, and centrifuge at 8000 r / min for 5 min.

[0051] Step 3. Take 10 mL of the supernatant, add 100 mg of magnetic surface molecular imprinting adsorbent, and shake and extract for 30 min. The magnetic surface molecular imprinting adsorbent was gathered to the bottom with a magnet, all the solution was poured carefully, and the adsorbent was washed twice with acetonitrile-water (1:4, V:V).

[0052] Step 4. Transfer the adsorbent to a 2mL centrifuge tube, add 1mL acetonitrile solution and vortex for 2min for desorption, ...

Embodiment 3

[0063] Example 3: A method for rapid detection of myclobutanil residues in food and the magnetic surface molecular imprinting adsorbent used therefor A rapid detection method for myclobutanil residues in peppers, the steps include:

[0064] Step 1. Homogenize the pepper sample to 200 mesh.

[0065] Step 2. Weigh 2.0 g of the sample and add it to a 50 mL centrifuge tube, then add 20 mL of acetonitrile-water (1:4, V:V), vortex and oscillate for extraction for 3 min, and centrifuge at 8000 r / min for 5 min.

[0066] Step 3. Take 10 mL of the supernatant, add 100 mg of magnetic surface molecular imprinting adsorbent, and shake and extract for 30 min. The magnetic surface molecular imprinting adsorbent was gathered to the bottom with a magnet, all the solution was poured carefully, and the adsorbent was washed twice with acetonitrile-water (1:4, V:V).

[0067] Step 4. Transfer the adsorbent to a 2mL centrifuge tube, add 1mL acetonitrile solution and vortex for 2min for desorption, ...

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Abstract

The invention discloses a method for rapidly detecting myclobutanil residues in food and a magnetic surface molecular imprinting adsorbent used by the method. The method comprises the following steps: (1) extraction: weighing a proper amount of sample, and adding acetonitrile-water to extract the myclobutanil residues; (2) enrichment and purification: taking a proper amount of the extracting solution, adding a magnetic surface molecular imprinting adsorbent to enrich and purify myclobutanil in the extracting solution, carrying out magnetic separation, washing with acetonitrile-water to remove impurities on the surface of the adsorbent, then adding an acetonitrile solution for desorption, blow-drying a desorption solution in nitrogen, and redissolving to prepare a to-be-detected solution; and (3) Raman spectroscopy rapid detection: uniformly mixing a proper amount of the to-be-detected solution with the gold nanoparticle sol and the auxiliary enhancement reagent for portable Raman spectroscopy test. The method can be used for rapid, efficient and low-cost detection of myclobutanil in food such as fruits, vegetables, cereals, tea leaves and fruit and vegetable juice.

Description

technical field [0001] The invention belongs to the technical field of enrichment, purification and detection of pesticide residues in food and environmental samples, and in particular relates to a method for rapidly detecting myclobutanil residues in food and a magnetic surface molecular imprinting adsorbent used therein. Background technique [0002] Pesticide residue analysis is an analytical technique for trace components in complex mixtures. Less sample consumption, less reagent consumption and faster analysis are the development trend of pesticide chemical pollutant residue detection technology. [0003] At present, the detection methods of pesticide residues mainly include enzyme inhibition method, gas chromatography, high performance liquid chromatography, gas chromatography mass spectrometry, liquid chromatography tandem mass spectrometry and so on. The operation steps of these detection methods are cumbersome, and the sample detection takes a long time. The disco...

Claims

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Application Information

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IPC IPC(8): G01N21/65C08F292/00C08F220/04C08F222/14C08J9/26
CPCG01N21/658C08F292/00C08J9/26C08J2351/10C08F220/04C08F222/102
Inventor 宋伟韩芳刘宇欣赵志慧王钰邓晓军伊雄海古淑青郭春丽吕亚宁周典兵丁磊郑平
Owner 合肥海关技术中心
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