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Process for immobilizing enzyme/microbe with polyvinyl alcohol

A technology of polyvinyl alcohol and immobilized enzymes, applied in the direction of protozoa, etc., can solve the problems of reduced mechanical strength and difficulty in forming immobilized particles, and achieve the effects of increased mechanical strength, improved water-soluble swelling, and enhanced ball forming ability

Inactive Publication Date: 2004-02-11
TSINGHUA UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] The purpose of the present invention is to solve the problems of difficult formation of immobilized particles in the existing polyvinyl alcohol immobilization technology and the reduction of mechanical strength due to water-soluble swelling in the use process, and propose a practical polyvinyl alcohol The method of immobilizing enzymes / microorganisms makes it easy to prepare, high mechanical strength of particles, and long service life

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Preparation steps:

[0023] (1) Mix 10g of polyvinyl alcohol, 0.5g of sodium alginate, 6.0g of acrylamide with 100mL of water, heat it in a water bath until it dissolves, then let it stand and cool down to room temperature;

[0024] (2) The wet weight of cellulase is 5 g, suspended in 10 mL of 0.9% NaCl, and mixed with 12.5 mL of the above solution.

[0025] (3) Add 1% CaCl to saturated boric acid solution 2 and 0.3% N, N'-methylenebisacrylamide to prepare Chengdu crosslinking agent solution;

[0026] (4) Use a 0.40mm ID syringe to add the mixed solution in (2) dropwise to the cooled crosslinking agent solution to form white spherical beads with a diameter of 2mm, soak them for 20h, and then wash with normal saline washing.

Embodiment 2

[0028] Preparation steps:

[0029] (1) Mix 15g of polyvinyl alcohol, 1.0g of sodium alginate, 4.0g of acrylamide with 100mL of water, heat it in a water bath until it dissolves, and then let it stand to cool down to room temperature;

[0030] (2) Trichosporium yeast cells with a wet weight of 5 g were suspended in 12 mL of 0.9% NaCl and mixed with 10 mL of the above solution.

[0031] (3) Add saturated boric acid solution to 2% CaCl 2 and 0.6% N, N'-methylenebisacrylamide to prepare Chengdu crosslinking agent solution;

[0032] (4) Add the mixture in (2) dropwise to the cooled cross-linking agent solution with a rubber straw to form white spherical beads with a diameter of 3 mm, soak them for 24 hours, and then wash them with physiological saline.

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Abstract

A process for preparing immobilized enzyme microbe with polyvinyl alcohol includes such steps as mixing polyvinyl alcohol, sodium algate and acrylamide with water, water-bath heating until full dissolving, then cooling to obtain solution of polyvinyl alcohol, mixing it with enzyme / microbe suspension according to a certain proportion, preparing solution of cross-linking agent by adding saturated boric acid solution to the mixture of calcium chloride and N,N'-methanobiacrylamide, dripping the said mixed solution to the solution of cross-linking agent to form white spherial particles, immersing for several hr, and washing with physiological brine. Its advantages are high mechanical strength and long service life.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular to a method for polyvinyl alcohol immobilizing enzymes / microorganisms. Background technique [0002] Polyvinyl alcohol (polyvinyl alcohol, referred to as PVA) is a new type of enzyme / microbe embedding and immobilization carrier, which has a series of advantages such as high strength, good chemical stability, strong anti-microbial decomposition performance, and low price. Embedding materials with practical potential have been extensively studied at home and abroad in recent years. [0003] Polyvinyl alcohol is soluble in water after heating, and gelation occurs after adding additives to its aqueous solution, or it is frozen at low temperature (-10°C) to form a gel, thereby embedding and fixing microorganisms in the gel network. Commonly used additives are boric acid and borax. Boric acid or borax is added to polyvinyl alcohol aqueous solution to form a gel through chemical reaction. The...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/10
Inventor 王建龙文湘华施汉昌钱易
Owner TSINGHUA UNIV
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