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Extraction and purification method of dendrobium officinale polysaccharide

A technology of Dendrobium officinale polysaccharide and purification method, applied in chemical instruments and methods, general water supply saving, membrane and other directions, can solve problems such as enrichment of Dendrobium officinale, and achieve the effects of improving yield, low heavy metal content and low cost

Pending Publication Date: 2021-12-17
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The present invention provides a method for extracting and purifying polysaccharides of Dendrobium candidum with simple operation, environmental protection, safety, high efficiency and low cost, and is suitable for industrial production. The problem of enrichment of toxic heavy metals in

Method used

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  • Extraction and purification method of dendrobium officinale polysaccharide
  • Extraction and purification method of dendrobium officinale polysaccharide
  • Extraction and purification method of dendrobium officinale polysaccharide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] ①. Take 200g of Dendrobium officinale powder and fully dissolve it in 1000mL of pure water to obtain the raw material liquid of Dendrobium officinale;

[0036] ②, Dendrobium candidum raw material liquid is passed through the microfiltration membrane with pore size of 0.1 μm to remove insoluble impurities (microfiltration conditions are temperature: 25 ℃, transmembrane pressure: 0.3MPa, solution flow rate 0.4L / min), carry out five microfiltrations in total, The retentate of each microfiltration was diluted with 1000mL pure water before the next microfiltration. A total of 5000 mL of permeate from five microfiltrations was combined;

[0037] ③. Pass 5000mL of the permeate in step ② through a 500kDa ultrafiltration membrane to extract the polysaccharide in it by dead-end ultrafiltration (ultrafiltration conditions are temperature: 25°C, transmembrane pressure: 0.3MPa, solution flow rate 0.4L / min), and the obtained After adding 1000mL pure water to the retentate, repeat th...

Embodiment 2-4

[0059] Change step 4. the concentration of NaCl aqueous solution is 1mol / L (embodiment 2), 1.5mol / L (embodiment 3), 2mol / L (embodiment 4), other operation is the same as embodiment 1, obtains the dendrobium officinale polysaccharide, obtained The quality, purity and heavy metal content of Dendrobium officinale polysaccharide are shown in Table 1 below:

[0060] Table 1

[0061]

[0062] a Step 4. the sodium chloride aqueous solution concentration that adds, b The concentration of NaCl in the solution in the dilute chamber after the end of electrodialysis.

Embodiment 5

[0064] Step 1.-3. are with embodiment 1;

[0065] ④ Add 300mL of the retentate collected by the ultrafiltration membrane into 1000mL of 0.5mol / L Na2SO4 aqueous solution and stir thoroughly, then pass through a 500kDa ultrafiltration membrane for dead-end ultrafiltration, and add 1000mL of the above-mentioned salt solution to the obtained retentate Repeat ultrafiltration, repeat ultrafiltration 3 times, collect retentate and permeate.

[0066] ⑤ Merging the permeate obtained in step ④ and step ③, the obtained permeate contains polysaccharides, oligosaccharides, monosaccharides, heavy metal salts and added sodium chloride. This solution is added in the dilute liquid tank of electrodialysis device (device schematic diagram sees image 3 ), add pure water in the concentrated liquid tank, and add 3% sodium sulfate aqueous solution in the polar liquid tank. The membrane stack of the electrodialysis device is composed of 5 groups of electrodialysis units. The cation exchange membra...

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Abstract

The invention discloses an extraction and purification method of dendrobium officinale polysaccharide, which comprises the steps of (1) fully dispersing dendrobium officinale powder in pure water to obtain a crude raw material solution; (2) removing insoluble impurities from the crude raw material solution through a microfiltration membrane to obtain a penetrating fluid 1 and a trapped fluid 1; (3) performing ultrafiltration treatment on the penetrating fluid 1 through a macroporous ultrafiltration membrane, and collecting a penetrating fluid 2 and a trapped fluid 2; (4) adding an aqueous solution of edible alkali metal inorganic salt into the trapped fluid 2, diluting, and performing ultrafiltration treatment through a macroporous ultrafiltration membrane to obtain a penetrating fluid 3 and a trapped fluid 3; (5) combining the penetrating fluid 2 and the penetrating fluid 3, adding into an electrodialysis device for desalting, and collecting a diluted chamber solution and a concentrated chamber solution; (6) carrying out ultrafiltration treatment on the diluted chamber solution through a small-hole ultrafiltration membrane, and collecting a trapped fluid 4 and a penetrating fluid 4; and (7) freeze-drying the trapped fluid 4 to obtain the dendrobium officinale polysaccharide. According to the method, the problem of enrichment of toxic heavy metals in the dendrobium officinale is solved while the extraction rate of the dendrobium officinale polysaccharide is increased.

Description

(1) Technical field [0001] The invention belongs to the technical field of extracting and purifying natural polysaccharides, and relates to a method for extracting and purifying dendrobium officinale polysaccharides. (2) Background technology [0002] Dendrobium has a long history in the field of medicinal health care in my country. The further processing of Dendrobium candidum is mostly to extract its main active ingredient-water-soluble polysaccharides, but some studies have shown that the polysaccharides in Dendrobium candidum that are partially dissolved in saline solution also have The neglect of salt-soluble polysaccharides in Dendrobium officinale has resulted in waste of resources and high processing costs due to anti-oxidation and anti-tumor effects. As a low-cost physical purification method, membrane separation technology does not introduce impurities and has mild operating conditions, which can effectively reduce the impact of processing on active ingredients. How...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
CPCC08B37/0003B01D61/423B01D61/149Y02A20/124B01D61/58B01D2311/12B01D2311/08C08L5/00B01D2311/2699B01D61/145B01D61/147B01D69/02B01D2317/025B01D2325/20
Inventor 邵平千佩玉潘杰峰
Owner ZHEJIANG UNIV OF TECH