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Immunofluorescence chromatography test strip for detecting mycoplasma lung antibody and application thereof

A chromatography test paper and immunofluorescence technology, which is applied in the field of immunofluorescence chromatography test paper detection, can solve the problems of small detection range and low sensitivity of test strips, and achieve the effects of easy promotion, high sensitivity, and reduced influence of interfering substances

Pending Publication Date: 2021-12-17
GUANGDONG LAB ANIMALS MONITORING INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The first purpose of the present invention is to provide an immunofluorescence chromatography test strip with higher sensitivity and a larger detection range for the problems of small detection range and low sensitivity of existing test strips

Method used

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  • Immunofluorescence chromatography test strip for detecting mycoplasma lung antibody and application thereof
  • Immunofluorescence chromatography test strip for detecting mycoplasma lung antibody and application thereof
  • Immunofluorescence chromatography test strip for detecting mycoplasma lung antibody and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] Embodiment 1 Preparation of an immunofluorescence chromatography test strip for detecting Mycoplasma pneumoniae antibody

[0058] Materials: nitrocellulose membrane, glass fiber membrane, absorbent paper, PVC bottom plate, film spraying gold spraying instrument, strip cutting instrument were purchased from Shanghai Jiening Biotechnology Co., Ltd.; staphylococcal protein A (SPA), goat anti-mouse IgG All were purchased from Suo Laibao Technology Co., Ltd.; carboxy fluorescent microspheres, 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC), N-hydroxysuccinimide (NHS) were purchased from Thermo Fisher Scientific Co., Ltd., etc. Mouse Mycoplasma pneumoniae ELISA kit was purchased from EBI-xpress Biotech International, USA.

[0059] The preparation method of mycoplasma pneumoniae antigen is as follows: Mycoplasma pneumoniae purchased from ATCC is inoculated in mycoplasma flesh culture medium at a ratio of 1:100, cultivated and harvested in 300mL mycoplasma cu...

Embodiment 2

[0064] Embodiment 2 Effect example

[0065] The detection principle of the fluorescent immunochromatographic test strip prepared in Example 1: After diluting the serum by 10 times with the sample diluent, take 100 μL of sample and add the sample, detect after 10 minutes, and record the T value and C value on the fluorescence quantitative analyzer .

[0066] 1. Linear range

[0067] The concentration of the detected sample can be calculated through the relationship between the fluorescence value and the concentration curve. The serum in this experiment is used as a real standard-building matrix to establish a standard curve, with lg (T / C) as the ordinate, and the self-defined standard concentration (TU / mL The logarithm of -1) is the abscissa, and the standard curve of the serum sample in the selected linear range of 10-640TU / mL is y=-0.2782x 2 +1.7544x-2.439, linear relationship R 2 is 0.9985, such as figure 1 . The results showed that the detection range of the fluorescen...

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Abstract

The invention discloses an immunofluorescence chromatography test strip for detecting a mycoplasma lung antibody and application thereof. The test strip comprises a bottom plate. A sample pad, a combination pad, a cellulose membrane and a water absorption pad are sequentially connected on the bottom plate. The combination pad is provided with a fluorescent microsphere labeled mycoplasma lung antigen. A detection T line coated with staphylococcus protein A and a quality control C line coated with goat anti-mouse IgG are arranged on the cellulose membrane. The invention relates to an immunofluorescence chromatography test strip for detecting mouse mycoplasma lung antibodies, which is established on the basis of taking fluorescent microspheres as a tracer. Compared with the traditional rapid detection technology, the test strip has the advantages of good sensitivity, specificity and stability, accurate and reliable detection result, wider detection range, simplicity and rapidness in operation, low cost and easiness in popularization, and the influence of interfering substances in a sample is reduced.

Description

technical field [0001] The invention belongs to the field of immunofluorescence chromatography test paper detection, and in particular relates to an immunofluorescence chromatography test strip for detecting pulmonary mycoplasma antibody and an application thereof. Background technique [0002] Mycoplasma (Mycoplasma pulmonis, referred to as MP) is a kind of microorganism in the genus Mycoplasma. Mycoplasma infection can cause severe diseases in experimental animals and mice. According to investigations, 60% of the barrier system and 100% of the mice raised in the normal environment May be infected with one or even a variety of mycoplasma. There are 5 kinds of mycoplasma isolated from mice and mice: Mycoplasma pulmonis, M. neurolytium, M. arthritidis, mouse mycoplasma Mycoplasma (M. muris) and Mycoplasma coli (M. collis). Among them, Mycoplasma murine can cause respiratory diseases in mice and mice, including rhinitis, otitis media, bronchitis and pneumonia. In addition, i...

Claims

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Application Information

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IPC IPC(8): G01N33/569G01N33/558G01N33/543G01N33/533G01N33/52
CPCG01N33/56933G01N33/558G01N33/54313G01N33/533G01N33/52G01N2333/30G01N2469/20
Inventor 朱余军丛锋伍妙梨黄碧洪练月晓罗银珠肖丽
Owner GUANGDONG LAB ANIMALS MONITORING INST
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