Composition of vitamin K2 and vitamin D3 and application thereof
A technology of vitamins and compositions, applied in the field of medicine, can solve problems such as the reduction of glycolysis of tumor cells, the limitation of anti-tumor research, and the oxidation of mitochondrial metabolic intermediates, so as to eliminate the increase of blood calcium, broaden the treatment methods, and expand the safe use range effect
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Embodiment 1
[0056] Embodiment 1 Vitamin K 2 Preparation of nanoemulsions
[0057] VK 2 Nanoemulsion is an oil-in-water nanoemulsion. VK 2 The recipe for nanoemulsion preparation is shown in Table 1, and the VK of the prescription amount is accurately weighed 2 , MCT, SPC, TPGS, and AP form the oil phase, and weigh the prescription amount of Na 2 S 2 o 5 , CaNa 2 Add appropriate amount of distilled water to EDTA and Gly to form the water phase. The oil phase was stirred and dissolved in a 60°C water bath, and the water phase was stirred and heated at 65°C. After the substances in the oil phase were completely dissolved, the water phase was slowly added to the oil phase under magnetic stirring conditions, and the stirring was continued for 25 minutes to obtain colostrum. The primary emulsion was homogenized four times at 800 bar using a high pressure homogenizer. Finally, the emulsion was filled into vials flushed with nitrogen, sealed with an aluminum cap, and autoclaved at 100 °...
Embodiment 2
[0060] Embodiment 2 Vitamin K 2 Characterization of Nanoemulsions
[0061] (1) Average diameter, polydispersity index (PDI)
[0062] Take the prepared VK 2 Appropriate amount of nanoemulsion, appropriate amount of sample, after diluting with sterile water for injection, put it into the sample pool, and use a laser particle size analyzer based on Nicomp-380 to measure. The results are shown in Table 2.
[0063] Table 2.VK 2 Particle size of nanoemulsion, PDI, (n=3)
[0064]
[0065] (2) Morphological identification under TEM scanning
[0066] Observation of VK by transmission electron microscopy (TEM, JM-1200EX, JEOL Ltd, Japan) 2 The shape of the nanoemulsion. The nanoemulsion was diluted with distilled water and dropped on a copper grid (300 mesh) covered with a carbon film. After the nanoemulsion adhered, phosphotungstic acid solution (2%, w / v) was added for staining for 2 min. Absorb excess liquid with filter paper, air-dry the sample overnight at room temperatur...
Embodiment 3
[0068] Embodiment 3 Oral high-dose vitamin K 2 Pharmacodynamic test of nanoemulsion for prevention and treatment of tumor
[0069] Oral high-dose VK via a xenograft mouse model of S180 tumors 2 (50mg / kg) tumor prevention efficacy research. The mice were randomly divided into 5 groups, 6 in each group, and the route of administration was oral gavage: the control group (0.9% normal saline, 10 mL·kg -1 ), A50 (oral VK before tumor bearing 2 Prevention 15 days 50mg·kg -1 ), B50 (oral VK before tumor bearing 2 Prevention for 15 days, oral VK after tumor bearing 2 15 days of treatment; 50mg·kg -1 ), C50 (no prevention before tumor bearing, oral VK immediately after tumor bearing 2 15 days of treatment; 50mg·kg -1 ), D50 (no prevention before tumor bearing, oral VK three days after tumor bearing 2 15 days of treatment; 50mg·kg -1 ). During the whole pharmacodynamic test period, record tumor volume, tumor mass, and plot the number of days; in order to better reflect the eff...
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