A pharmaceutical composition or cosmetic product for skin repair
A composition and skin technology, applied in the direction of drug combination, skin care preparations, skin diseases, etc., to achieve high antibacterial effect, promote skin repair, and good skin repair effect
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Embodiment 1
[0025] Example 1 Design and identification of antimicrobial peptides
[0026] Design of antimicrobial peptide library: The length of antimicrobial peptides is set in the range of 7-18 amino acids, and the common dominant amino acids of antimicrobial peptides are selected as the basis, and the N-terminal amino acid residue with relatively long half-life is used as the N-terminal amino acid. library. Based on three common pathogenic bacteria in burn treatment, burn wounds were infected with drug-resistant Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli for computer combined simulation screening, and 40 peptides were preliminarily screened for artificial synthesis.
[0027] Antibacterial peptide effect identification:
[0028] Staphylococcus aureus ATCC29213 (resistant bacteria, MRSA), Pseudomonas aeruginosa ATCC27853 (resistant bacteria, RPA, Escherichia coli ATCC25922 (resistant bacteria, RECO) were purchased from Nanjing Diaodian Biotechnology Co., Ltd.
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Embodiment 2
[0035] Example 2 Antioxidant activity polypeptide screening
[0036] In the process of burn treatment, in addition to inhibiting three pathogenic bacteria, antimicrobial peptides that can exhibit antioxidant activity are also beneficial for treatment. Therefore, peptides that can inhibit pathogenic bacteria and have antioxidant activity are selected.
[0037] The amount of reagent added in the DPPH determination process is shown in Table 2, wherein the sample to be tested is an emulsion of 90% 1 mg / mL prepared by using fatty acid sucrose ester for each polypeptide.
[0038] 10 Table 2 The amount of reagents added in the process of DPPH determination
[0039] various 11Ac Ad Ak DPPH 3mL - 3mL sample to be tested 1mL 1mL - Ethanol - 3mL - Sample solvent - - 1mL
[0040] Add the prepared reagents according to Table 2, shake well, react at room temperature for 30 min in the dark, and measure the absorbance at 517 nm. The ...
Embodiment 3
[0046] Example 3 Therapeutic experiment of SX-16 polypeptide in the treatment of scalded mouse model
[0047] Mice were anesthetized by intraperitoneal 10% water and chloral, 8% sodium sulfide back hair removal, routine disinfection. A self-made water-bath scalding board was used, and the boiling water was kept for 10 s. One round wound with a diameter of 0.85 cm was scalded on the left and right sides of the middle of the back lumbar vertebrae (the skin pathological section confirmed that it was a second-degree burn), and the two adjacent wounds were separated by 1 cm. Pseudomonas aeruginosa 0.05mL (1×108CFU / mL) was applied to the wound. The scalded model mice were randomly divided into 4 groups with 10 mice in each group. 0.05mL of polypeptide (50mg / L) was evenly instilled on the wounds of the experimental group at 2 h after injury, and 0.05mL of PBS, hEGF (100μg / L) and 0.05mL (50mg / L) of strong burn and scald ointment were instilled in the control group respectively. , Af...
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