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Microbial complex bacterial agent and preparation method and application thereof

A technology of compound bacterial agent and microorganism, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of easy death of bacteria and general treatment effect, achieve treatment effect and efficiency improvement, and improve treatment effect , highly active effect

Pending Publication Date: 2021-12-31
南通华宇化工科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The object of the present invention is to provide a kind of microbial composite bacterial agent and its preparation method and application, to solve the existing microbial composite bacterial agent proposed in the above-mentioned background technology to the treatment effect of the organic waste water containing toluene, methylene chloride general, strain Death-prone technical issues

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] A microbial compound bacterial agent, including Lactobacillus buchneri (lactobacillus buchneri) with a preservation number of ATCC No. 11307, Pichia sp. with a preservation number of ATCC No. 28487, belonging to PNSB and a preservation number of ATCC Rhodospirillum rubrum NO.11170, Rhodopseudomonas palustris with CICC NO.23812, Actinosporangium sp. with ATCC NO.31127 , Rhizopus oligosporus with the preservation number ATCC NO.22959, Halobacterium sp. with the preservation number ATCC NO.700922, and Pseudonocardia benzoate with the preservation number DSM NO.44703 (Pseudonocardia benzonivorans), its CFU ratio is 1:2:5:4:3:3:4:5.

[0028] The preparation of above-mentioned microbial composite bacterial agent:

[0029] S1. Inoculate Lactobacillus buchneri, Pichia pastoris, actinomyces cysts, Rhizopus oligosporus, Bacillus halophilus and Pseudonocardia benzobivorans respectively in the first low temperature stress medium, 80rpm / min, Cultivate for 72 hours at 28°C; inocula...

Embodiment 2

[0036] The microbial compound bacterial agent of the present embodiment includes Lactobacillus brucei, Pichia pastoris, Rhodospirillum rubrum, Rhodopseudomonas palustris, actinomycetes cyst, Rhizopus oligosporus, Bacillus halophilus and benzene-eating bacteria Pseudonocardia, the CFU ratio is 2:3:6:5:4:4:5:6, the total CFU concentration is 5×10 8 CFU / mL.

[0037] All the other preparation methods and applications are the same as in Example 1.

Embodiment 3

[0039] The microbial complex bacterial agent in this embodiment includes Lactobacillus brucella, Pichia pastoris, Rhodospirillum rubrum, Rhodopseudomonas palustris, cyst actinomycetes, Rhizopus oligosporus, Bacillus halophilus and food Pseudonocardia benzene, the CFU ratio is 1:2.5:5:4.5:3:3.5:4:5.5, the total CFU concentration is 5×10 8 CFU / mL.

[0040] All the other preparation methods and applications are the same as in Example 1.

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Abstract

The invention discloses a microbial complex bacterial agent and a preparation method and application thereof. The microbial complex bacterial agent is prepared by mixing lactobacillus buchneri, pichia pastoris, rhodospirillum rubrum, rhodopseudomonas palustris, sporocyst actinomyces, rhizopus oligosporus, halophilic bacillus and pseudonocardia benzene according to a CFU ratio of (1-2): (2-3): (5-6): (4-5): (3-4): (3-4): (4-5): (5-6). The lactobacillus buchneri, the pichia pastoris, the rhodospirillum rubrum, the rhodopseudomonas palustris, the sporocyst actinomyces, the rhizopus oligosporus, the halophilic bacillus and the pseudonocardia benzene in the microbial complex bacterial agent are mutually coordinated and matched to form a complete symbiotic and syntrophic system, and an organic solvent in sewage can be efficiently treated.

Description

technical field [0001] The invention relates to the technical field of sewage treatment, in particular to a microbial composite bacterial agent and its preparation method and application. Background technique [0002] At present, the common treatment methods of organic wastewater include physical treatment, chemical treatment and biological treatment; among them, the biological treatment is to treat organic wastewater through microbial compound bacteria agent, that is, to use the growth and reproduction of microorganisms to decompose and oxidize organic matter, and to restore the water body. Self-purification ability, its application is more and more widely, but because organic wastewater contains not only aerobic organic matter, but also heavy metals, plant nutrients (ammonia nitrogen, etc.), and toxic and harmful organic matter, it is extremely difficult to treat, most of the biological treatment methods The effect is not very ideal. [0003] In response to the above prob...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/16C12N1/14C02F3/34C12R1/225C12R1/84C12R1/845C12R1/07C12R1/01C02F101/32C02F101/36
CPCC12N1/20C12N1/16C12N1/14C02F3/341C02F3/347C02F2101/322C02F2101/36
Inventor 彭冬兵朱信良李维宏
Owner 南通华宇化工科技有限公司