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Cordyceps javanica strain Bd01 and application thereof

A technology of Cordyceps and strains, which is applied in the field of agricultural microorganisms, can solve the problem of fewer microorganisms, and achieve the effect of reducing the impact and benefiting the yield and quality

Active Publication Date: 2022-01-04
云南绿戎生物产业开发股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because microorganisms can be prepared into highly efficient, safe, and residue-free biopesticides, they have been vigorously pursued by researchers and have also brought good news to consumers. Although there are reports of microorganisms used to control coleopteran pests, they can be used efficiently at present. There are very few microorganisms for the control of the longhorn beetle, and there are few reports on the use of Cordyceps javanica to control the longhorn beetle

Method used

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  • Cordyceps javanica strain Bd01 and application thereof
  • Cordyceps javanica strain Bd01 and application thereof
  • Cordyceps javanica strain Bd01 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Isolation, Purification and Identification of Cordyceps Javanese Bd01 Strain

[0033] 1. Isolation and purification of bacterial strains

[0034] It was isolated from the dead beetle larvae of the coffee beetle larvae, which died naturally. microbial pesticides. Adopt the following steps to carry out separation and purification:

[0035] 1. First, rinse the larvae of the beetle longicorn beetle that died of the disease with 75% alcohol, then rinse 3 times with sterile water, and finally dry the water with sterilized filter paper for later use.

[0036] 2. Sterilization: All experimental equipment such as petri dishes, centrifuge tubes, test tubes, and pipette tips and sterile water are sterilized by high pressure at 0.1MPa, 121°C, and sterilized for 30 minutes.

[0037] 5. Preparation of culture medium

[0038] Medium for separation and purification: PDA medium, including: 200g of peeled potatoes, cut into pieces and boiled with water for 20min, filtered through dou...

Embodiment 2

[0067] The influence of embodiment 2 different environmental conditions on bacterial strain vegetative growth

[0068] The Cordyceps javanica strain was inoculated on PDA medium and cultured for 2 weeks for the optimization of the following conditions.

[0069] 1. Effects of different media on vegetative growth and sporulation of Cordyceps javanica strain

[0070] The strains were cultured in five mediums of PDA, PPDA, SDAY, SMAY, and Czapek, and the colony diameter was measured every 2 days for a total of 10 days. At the same time, the initial sporulation time recorded on different media was observed, and the sporulation was measured after 14 days. quantity. Use a hole puncher with a diameter of 5 mm to take 3 colonies (at the same position as the center of the petri dish) and put them in 2 mL of sterile water containing 0.1% Tween-80, shake them fully, measure the number of spores with a hemocytometer, and calculate the amount of sporulation. Select the most suitable mediu...

Embodiment 3

[0088] The influence of embodiment 3 different environmental conditions on bacterial strain pathogenicity

[0089] Preparation of spore suspension: according to the screening conditions in Example 2, the Cordyceps javanica strain Bd01 was inoculated in PPDA medium, and cultured in a constant temperature incubator at 26°C for 2 weeks;

[0090] The Cordyceps javanica strain Bd01 that has been cultured for 2 weeks and completely sporulated was washed out with sterile water containing 0.1% Tween-80, stirred evenly with a magnetic stirrer, counted by a hemocytometer, and prepared into a spore suspension of the required concentration.

[0091] 1. Pathogenicity of strains at different temperatures

[0092] Select 20 healthy long-tailed beetle larvae with the same shape and size, and soak them at a concentration of 1×10 8 After 30 seconds in the spore / mL spore suspension, insert into a finger tube (1.2×5 cm), and repeat each treatment 3 times. Put them into the incubator with the ab...

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Abstract

The invention discloses a cordyceps javanica strain Bd01 and application thereof, and belongs to the technical field of agricultural microorganisms. The Cordyceps javanica strain Bd01 is preserved in the China General Microbiological Culture Collection Center on August 16, 2021, the preservation number of the Cordyceps javanica strain Bd01 is CGMCC No.23078, and the preservation address of the Cordyceps javanica strain Bd01 is Institute of Microbiology, Chinese Academy of Sciences, No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing. The cordyceps javanica strain Bd01 is separated from dead caffeine larva bombyx batryticatus bodies, a living body of the strain or a spore suspension of the strain is used as an active ingredient to prepare a biological preparation, the aim of preventing and treating caffeine caffeine and diseases caused by caffeine caffeine is achieved, and the yield and the quality of coffee can be improved.

Description

technical field [0001] The invention relates to the technical field of agricultural microorganisms, in particular to a Cordyceps javanica strain Bd01 and its application. Background technique [0002] The coffee tree is a perennial evergreen shrub or small tree belonging to Rubiaceae. The coffee beans used in daily drinking coffee are derived from the nuts in the fruit of the coffee tree. With the change of people's consumption concept, people's demand for coffee is increasing year by year. Yunnan Province, as the main producing area of ​​coffee arabica in China, supplies the coffee needed for all parts of the country and trade and export. However, due to the single and large-scale planting of coffee arabica in recent years, the pests that harm coffee have formed a stable ecosystem, which is seriously serious. Harm the yield and quality of coffee, and have a certain impact on market demand. There are many kinds of pests that harm coffee. There are more than 360 kinds of pes...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/30A01P7/04C12R1/645
CPCC12N1/14A01N63/30
Inventor 吴国星刘全俊秦小萍易璟贾奔高熹何明川李金梁兰明先施春兰
Owner 云南绿戎生物产业开发股份有限公司
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